Estradiol increases the duration of nuclear androgen receptor occupation in the preoptic area of the male rat treated with dihydrotestosterone

Roselli, Charles E.; Fasasi, Taiwo A.

doi:10.1016/0960-0760(92)90024-d

Cited by 35 publications

(11 citation statements)

References 54 publications

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“…In peripheral tissues, estrogen has been shown to antagonize some of the cellular effects of androgens [15]. In brain, E 2 can increase AR concentration [10] and significantly augment the duration of AR occupation [11]. Our study demonstrated the presence of three types of hormone-sensitive neurons in male rat brain.…”

Section: Discussionmentioning

confidence: 72%

“…In addition, estrogen increases the number of androgen receptors (AR) and the duration of ligand occupancy [10, 11] within brain regions involved in the control of mating, and androgens downregulate the expression of ER [12, 13], suggesting an interaction between both steroids. Depending on the tissue, androgenic and estrogenic interactions may be either antagonistic or synergistic [14, 15].…”

Section: Introductionmentioning

confidence: 99%

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Androgen Receptors and Estrogen Receptors Are Colocalized in Male Rat Hypothalamic and Limbic Neurons that Express Fos Immunoreactivity Induced by Mating

et al. 1998

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Conversion of testosterone into estradiol is important for male rat sexual behavior, and both steroids probably contribute to mating. The distributions of neurons containing androgen receptors (AR) and estrogen receptors (ER) overlap, and many AR-immunoreactive (AR-ir) neurons express Fos immunoreactivity (Fos-ir) induced by mating. Because mating-induced Fos-ir in the male rat occurs mainly in AR-ir neurons, and because both steroids are important for mating, we hypothesized that (i) AR-ir and ER-ir are colocalized and that (ii) some of these neurons are activated during mating. We examined, in adjacent sections from the medial preoptic area (MPN) through the central tegmental field (CTF), the expression of ER-ir in: (i) AR-ir-containing neurons, and (ii) Fos-ir-expressive neurons. PG21 anti-AR, OA-11-824 anti-c-fos, H222 or 1D5 anti-ER primary antibodies were visualized, respectively, with cyanine-conjugated, fluorescein- or cyanine-conjugated, and fluorescein-conjugated secondary antibodies in male rats which were killed 1 h after ejaculating with a receptive female. In MPN, bed nucleus of the stria terminalis (BNST), and medial amygdala (MEA), 80–90% of ER-ir labeling occurred in AR-ir-positive neurons but only about 30% of AR-ir neurons were ER-ir-positive. No ER-ir was found in the CTF. This suggests the presence of three types of brain neurons sensitive to gonadal steroid hormones: neurons sensitive to androgens only, neurons sensitive to both androgens and estrogens, and neurons sensitive to estrogens only. About 50% of ER-ir labeling occurred in cells expressing mating-induced Fos-ir but only about 30% of Fos-ir neurons were ER-ir-positive. These findings suggest that, in the MPN, at least two different neuronal populations are activated during mating: the first contains AR-ir only and the second contains AR-ir and ER-ir. In the BNST and MEA, at least three hormonally sensitive populations are activated during mating: the two described above plus a third population which expresses ER-ir only.

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Section: Discussionmentioning

confidence: 72%

Section: Introductionmentioning

confidence: 99%

Androgen Receptors and Estrogen Receptors Are Colocalized in Male Rat Hypothalamic and Limbic Neurons that Express Fos Immunoreactivity Induced by Mating

et al. 1998

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“…Sodersten 1980). Estrogen might also increase the ef fectiveness of androgen receptors of vasopressin-producing cells by altering the duration of androgen receptor occupation, as has been observed in the preoptic area of male rats (Roselli and Fasasi 1992). Sex-related differences in androgen levels cannot fully explain sex-related dif ferences in vasopressin fiber staining and vasopressin mRNA levels observed in intact male and female rats (De Vries et al 1981;Miller et al 1989b).…”

Section: Cellular Basis Of Sex-related Differences In Vasopressin Expmentioning

confidence: 99%

Studying neurotransmitter systems to understand the development and function of sex differences in the brain: the case of vasopressin

Vries¹,

Geert²

1995

Neurobiological Effects of Sex Steroid Hormones

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“…Castration followed by hormone replacement disrupts normal homeostatic mechanisms regulating steroid hormone activity, and the potential exists for treatmentinduced activation of compensatory mechanisms not associated with normal development. For example, administration of estrogens alters androgen receptor concentration (Handa et al, 1987a,b), increases nuclear retention time of occupied androgen receptors (Roselli and Fasasi, 1992), and interferes with dihydrotestosterone catabolism (Soderstein and Gustafsson, 1980;Soderstein et al, 1986). Thus, it is possible that the observed trophic effects of estrogens on SNB dendritic development in castrated, estradiol/dihydrotestosterone-treated males may actually represent enhancement of androgenic activity induced by the presence of pharmacological doses of estradiol.…”

Section: Introductionmentioning

confidence: 97%

Ovariectomy attenuates dendritic growth in hormone‐sensitive spinal motoneurons

2001

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The lumbar spinal cord of rats contains the sexually dimorphic, steroid-sensitive spinal nucleus of the bulbocavernosus (SNB). Dendritic development of SNB motoneurons in male rats is biphasic, initially showing exuberant growth through 4 weeks of age followed by a retraction to mature lengths by 7 weeks of age. The initial growth is steroid dependent, attenuated by castration or aromatase inhibition, and supported by hormone replacement. Dendritic retraction is also steroid sensitive and can be prevented by testosterone treatment, but is unaffected by aromatase inhibition. Together, these results suggest a role for estrogens during the initial growth phase of SNB development. In this study, we tested whether ovarian hormones could support SNB somal and dendritic development. Motoneuron morphology was assessed in normal males and in females perinatally masculinized with dihydrotestosterone and then either ovariectomized or left intact. SNB motoneurons were retrogradely labeled with cholera toxin-HRP at 4 or 7 weeks of age and reconstructed in three dimensions. Initial growth of SNB dendrites was reduced after ovariectomy in masculinized females. However, no differences in dendritic length were seen at 7 weeks of age between intact and ovariectomized masculinized females, and lengths in both groups were significantly lower than those of normal males. Together with previous findings, these results suggest that estrogens are involved in the early growth of SNB dendrites, but not in their subsequent retraction.

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Estradiol increases the duration of nuclear androgen receptor occupation in the preoptic area of the male rat treated with dihydrotestosterone

Cited by 35 publications

References 54 publications

Androgen Receptors and Estrogen Receptors Are Colocalized in Male Rat Hypothalamic and Limbic Neurons that Express Fos Immunoreactivity Induced by Mating

Androgen Receptors and Estrogen Receptors Are Colocalized in Male Rat Hypothalamic and Limbic Neurons that Express Fos Immunoreactivity Induced by Mating

Studying neurotransmitter systems to understand the development and function of sex differences in the brain: the case of vasopressin

Ovariectomy attenuates dendritic growth in hormone‐sensitive spinal motoneurons

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