Estradiol In Vivo Induces Changes in Cardiomyocytes Size in Obese Rats

Obradović, Milan; Sudar-Milovanović, Emina; Zafirović, Sonja; Stanimirović, Julijana; Labudović‐Borović, Milica; Isenović, Esma R.

doi:10.1177/0003319713514477

Cited by 27 publications

(29 citation statements)

References 73 publications

(112 reference statements)

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“…E 2 has a direct effect on the heart by influencing lipid metabolism and insulin sensitivity (Koricanac et al 2012). Furthermore, in the same rat model as used in this study we have previously reported that in vivo the administration of E 2 as a bolus injection reduced cardiac hypertrophy, decrease serum total cholesterol and high sensitivity C-reactive protein, and realigning of insulin signalling cascade (Obradovic et al 2015a, Obradovic et al 2015b). …”

Section: A C C E P T E D Accepted Manuscriptmentioning

confidence: 53%

“…We have previously shown that the plasma (Obradovic et al 2015a) and liver lysate FFA concentrations (Stanimirovic et al 2016) are significantly higher in HF fed IR male rats compared with controls, and that in vivo E 2 treatment has no effect on HF fed male rats plasma M A N U S C R I P T…”

Section: Effect Of Estradiol On Ffa Concentratons In Heart Lysates Frmentioning

confidence: 97%

“…We chose this experimental rat model based on our previous results, and literature data (Barnard et al 1998, Obradovic et al 2015b). All metabolic parameters for these rats in response to the treatments administered in this study are detailed in our previous study (Obradovic et al 2015a). The rats were kept at a 12:12 h, light/dark cycle at 22±2°C and divided into two groups: one group (labelled as CONT) was fed for 10 weeks with a balanced diet for laboratory rats (prepared by Veterinarski zavod Subotica, Subotica, Serbia); and a second group (labeled as HF) was fed with a HF diet, a balanced diet for laboratory rats enriched with 42% fat.…”

Section: Animals and Experimental Treatmentmentioning

confidence: 99%

“…In this model the HF diet induces whole body IR/hyperinsulinemia, endothelial dysfunction and hypertension (Barnard et al 1998, Panchal et al 2011. Furthermore, we have previously demonstrated using the same male model rats, that a HF diet applied for 10 weeks induces obesity accompanied with abnormalities such as cardiac hypertrophy, increased serum insulin concentration, whole body IR, hyperlipidaemia, hyperleptinemia and inflammation (Obradovic et al 2015a, Obradovic et al 2015b). …”

Section: Introductionmentioning

confidence: 96%

“…FFA concentration (Obradovic et al 2015a). Here we further examined the effect of a HF diet in combination with E 2 treatment on the FFA concentration in heart lysates (Figure 1).…”

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confidence: 99%

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17β-Estradiol protects against the effects of a high fat diet on cardiac glucose, lipid and nitric oxide metabolism in rats

Zafirović

Obradović

Sudar-Milovanović

et al. 2017

Molecular and Cellular Endocrinology

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Please cite this article as: Zafirovic, S., Obradovic, M., Sudar-Milovanovic, E., Jovanovic, A., Stanimirovic, J., Stewart, A.J., Pitt, S.J., Isenovic, E.R., 17β-Estradiol protects against the effects of a high fat diet on cardiac glucose, lipid and nitric oxide metabolism in rats, Molecular and Cellular Endocrinology (2017), doi: 10.1016/j.mce.2017 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. AbstractThe aim of this study was to investigate the in vivo effects of estradiol (E 2 ) on myocardial metabolism and inducible nitric oxide synthase (iNOS) expression/activity in obese rats. MaleWistar rats were fed with a normal or a high fat (HF) diet (42% fat) for 10 weeks. Half of the HF fed rats were treated with a single dose of E 2 while the other half were placebo-treated. 24h after treatment animals were sacrificed. E 2 reduced cardiac free fatty acid (FFA Akt, protein kinase B; AS160, Akt substrate of 160 kDa; CD36, fatty acid transporters; E 2 ,

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Section: A C C E P T E D Accepted Manuscriptmentioning

confidence: 53%

Section: Effect Of Estradiol On Ffa Concentratons In Heart Lysates Frmentioning

confidence: 97%

Section: Animals and Experimental Treatmentmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 96%

“…FFA concentration (Obradovic et al 2015a). Here we further examined the effect of a HF diet in combination with E 2 treatment on the FFA concentration in heart lysates (Figure 1).…”

mentioning

confidence: 99%

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17β-Estradiol protects against the effects of a high fat diet on cardiac glucose, lipid and nitric oxide metabolism in rats

Zafirović

Obradović

Sudar-Milovanović

et al. 2017

Molecular and Cellular Endocrinology

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Estradiol‐mediated regulation of hepatic iNOS in obese rats: Impact of Src, ERK1/2, AMPKα, and miR‐221

Panic

Stanimirović

Obradović

et al. 2018

Biotech and App Biochem

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This study aimed to investigate in vivo effects of estradiol on the regulation of hepatic inducible nitric oxide synthase (iNOS) expression in the high fat (HF) diet-induced obesity. Also, we aimed to investigate whether activation of the extracellular signal-regulated kinase (ERK1/2), adenosine monophosphate-activated protein kinase (AMPK), Src kinase, and miR-221 is involved in estradiol-mediated regulation of iNOS in the liver of obese male Wistar rats. Male Wistar rats were fed a standard laboratory diet or a HF diet for 10 weeks. Half of HF rats were treated with estradiol intraperitoneally (40 μg/kg), whereas the other half were placebo-treated 24 H before euthanasia. Results show that estradiol treatment of HF rats decreased hepatic iNOS mRNA (P < 0.05) and protein expression (P < 0.01), the protein levels of p65 subunit of nuclear factor κB (P < 0.05) and ERα (P < 0.05), ERK1/2 phosphorylation (P < 0.001), and ERα/Src kinase association (P < 0.05). By contrast, hepatic Src protein level (P < 0.05), AMPKα phosphorylation (P < 0.05), and miR-221 expression (P < 0.05) were increased in HF rats after estradiol treatment. Our results indicate that estradiol in vivo regulates hepatic iNOS expression in obese rats via molecular mechanisms involving ERK1/2, AMPK, Src, and miR-221 signaling.

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