Changes in land use interfere with river basin connectivity, aff ecting the fl ow dynamics, sediment yield, and density of river connections with roads. In this research we studied the variation of land use from 1980-2002-2016 using vector mappings in the classes: temporary crops, primary and secondary forests; pasture, forestry, buildings, ponds, and the development of crossings between drainage roads (road-stream crossings) in the Guabiroba River basin. Variations in the use and percentages over the 36 years studied were observed. There was a reduction in primary forest and pasture areas and an increase in planted forests and temporary crops. Regarding the crossings, there was an increase in the topographic dimensions and in sectors with sharp slopes and fi rst-order channels. Over the years, the uses in the basin were alternated according to the productive structures, showing needs according to the creation, modifi cation, and suppression of roads. In temporary crops, the period from planting to harvesting is up to 6 months, and slash-and-burn agriculture rage from 3 to 5 years between each planting, and forestry depending on the demands it can range from 4 to 20 years. These variations in time and space, interfere with the productive fl ows and consequently with the road-stream crossings. Resumo: As mudanças no uso da terra interferem na conectividade das bacias hidrográfi cas, afetando a dinâmica de fl uxos, a produção de sedimentos e a densidade de conexões de ríos com estradas. Esta pesquisa visa evidenciar como o uso da terra Informações sobre o Artigo Recebido (