Estimations of topographically correct regeneration to nerve branches and skin after peripheral nerve injury and repair

Puigdellı́vol-Sánchez, Anna; Prats-Galino, Alberto; Molander, Carl

doi:10.1016/j.brainres.2006.04.077

Cited by 13 publications

(9 citation statements)

References 55 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In addition, the size of the nerve used for sequential tracing must be considered. Recently, Puigdellivol-Sanchez et al (24) found a much higher percentage of correctly directed tibial motoneurons after direct coaptation repair (87%) using the same technique and time point of evaluation. The difference with our results might be explained by the difference in repair techniques (direct coaptation vs graft repair (7) and by the larger size of the tibial motoneuron pool.…”

Section: Discussionmentioning

confidence: 96%

Accuracy of Motor Axon Regeneration Across Autograft, Single-Lumen, and Multichannel Poly(lactic-Co-Glycolic Acid) Nerve Tubes

et al. 2008

View full text Add to dashboard Cite

Objective Accuracy of motor axon regeneration becomes an important issue in the development of a nerve tube for motor nerve repair. Dispersion of regeneration across the nerve tube may lead to misdirection and polyinnervation. In this study, we present a series of methods to investigate the accuracy of regeneration, which we used to compare regeneration across autografts and single lumen poly(lactic-co-glycolic acid) (PLGA) nerve tubes. We also present the concept of the multichannel nerve tube that may limit dispersion by separately guiding groups of regenerating axons. Methods Simultaneous tracing of the tibial and peroneal nerves with fast blue (FB) and diamidino yellow (DY), 8 weeks after repair of a 1-cm nerve gap in the rat sciatic nerve, was performed to determine the percentage of double-projecting motoneurons. Sequential tracing of the peroneal nerve with DY 1 week before and FB 8 weeks after repair was performed to determine the percentage of correctly directed peroneal motoneurons. Results In the cases in which there was successful regeneration across single lumen nerve tubes, more motoneurons had double projections to both the tibial and peroneal nerve branches after single lumen nerve tube repair (21.4%) than after autograft repair (5.9%). After multichannel nerve tube repair, this percentage was slightly reduced (16.9%), although not significantly. The direction of regeneration was nonspecific after all types of repair. Conclusion Retrograde tracing techniques provide new insights into the process of regeneration across nerve tubes. The methods and data presented in this study can be used as a basis in the development of a nerve tube for motor nerve repair.

show abstract

Section: Discussionmentioning

confidence: 96%

Accuracy of Motor Axon Regeneration Across Autograft, Single-Lumen, and Multichannel Poly(lactic-Co-Glycolic Acid) Nerve Tubes

et al. 2008

View full text Add to dashboard Cite

show abstract

“…It is also known that transected nerves may regenerate following nerve injury (Imai et al 2003;Jungnickel et al 2006;Lozeron et al 2004;Lundborg and Rosen 2007;Puigdellivol-Sanchez et al 2006;Rodger et al 2006). Anatomical studies have reported that the injured IAN begins to reinnervate peripheral structures within 7 days after nerve injury in rats (Atsumi et al 2000;Harada et al 2003;Youn et al 1997).…”

Section: Introductionmentioning

confidence: 99%

Modulation of Trigeminal Spinal Subnucleus Caudalis Neuronal Activity Following Regeneration of Transected Inferior Alveolar Nerve in Rats

Saito

Hitomi²,

Suzuki³

et al. 2008

Journal of Neurophysiology

View full text Add to dashboard Cite

Modulation of trigeminal spinal subnucleus caudalis neuronal activity following regeneration of transected inferior alveolar nerve in rats. To clarify the neuronal mechanisms of abnormal pain in the face innervated by the regenerated inferior alveolar nerve (IAN), nocifensive behavior, trigeminal ganglion neuronal labeling following Fluorogold (FG) injection into the mental skin, and trigeminal spinal subnucleus caudalis (Vc) neuronal properties were examined in rats with IAN transection. The mechanical escape threshold was significantly higher at 3 days and lower at 14 days after IAN transection, whereas head withdrawal latency to heat was significantly longer at 3, 14, and 60 days after IAN transection. The number of FG-labeled ganglion neurons was significantly reduced at 3 days after IAN transection but increased at 14 and 60 days. The number of wide dynamic range (WDR) neurons with background (BG) activity was significantly higher at 14 and 60 days after IAN transection compared with naïve rats, and the number of WDR and low-threshold mechanoreceptive (LTM) neurons with irregularly bursting BG activity was increased at these two time points. Mechanically evoked responses were significantly larger in WDR and LTM neurons 14 days after IAN transection compared with naïve rats. Heat- and cold-evoked responses in WDR neurons were significantly lower at 14 days after transection compared with naïve rats. Mechanoreceptive fields were also significantly larger in WDR and LTM neurons at 14 and 60 days after IAN transection. These findings suggest that these alterations may be involved in the development of mechanical allodynia in the cutaneous region innervated by the regenerated IAN.

show abstract

“…Branches of the sciatic nerve are increasingly being used in the study of nerve injury and neuronal survival (Hu and McLachlan 2003;Oaklander and Brown 2004;Puigdellivol-Sanchez et al 2006;Welin et al 2008). Despite several studies examining the neurochemistry of cutaneous and muscular sensory aVerents (Ambalavanar et al 2003;Lawson et al 2002;McLachlan and Janig 1983;Molander et al 1987;O'Brien et al 1989), those properties of the medial gastrocnemius and sural neurons in the rat have not yet been clearly characterised.…”

Section: Discussionmentioning

confidence: 99%

Phenotype of distinct primary sensory afferent subpopulations and caspase-3 expression following axotomy

Reid

Mantovani

Shawcross

et al. 2011

Histochem Cell Biol

View full text Add to dashboard Cite

Specific sensory neuronal subpopulations show contrasting responses to peripheral nerve injury, as shown by the axotomy-induced death of many cutaneous sensory neurons whilst muscular sensory afferents survive an identical insult. We used a novel combination of retrograde neuronal tracing with immunohistochemistry and laser microdissection techniques, in order to describe the neurochemistry of medial gastrocnemius (muscular sensory afferents) and sural (cutaneous sensory afferents) branches of the rat sciatic nerve and relate this to the pro-apoptotic caspase-3 gene expression following nerve transection. Our results demonstrated distinctions in medial gastrocnemius and sural neuron populations with the most striking difference in the respective proportions of isolectin B4 (IB4) staining neurons (3.7 V 32.8%). The mean neuronal area of the medial gastrocnemius (MG) neurons was larger than that of the sural (SUR) neurons (1,070.8 V 646.2 μm²) and each phenotypic group was significantly smaller in sural neurons than in MG neurons. At 1 week post-axotomy, MG neurons markedly downregulated caspase-3, whilst SUR neurons upregulated caspase-3 gene expression; this may be attributable to the differing IB4-positive composition of the subpopulations. These findings provide further clarification in the understanding of two distinct neuronal populations used increasingly in nerve injury models.

show abstract

Estimations of topographically correct regeneration to nerve branches and skin after peripheral nerve injury and repair

Cited by 13 publications

References 55 publications

Accuracy of Motor Axon Regeneration Across Autograft, Single-Lumen, and Multichannel Poly(lactic-Co-Glycolic Acid) Nerve Tubes

Accuracy of Motor Axon Regeneration Across Autograft, Single-Lumen, and Multichannel Poly(lactic-Co-Glycolic Acid) Nerve Tubes

Modulation of Trigeminal Spinal Subnucleus Caudalis Neuronal Activity Following Regeneration of Transected Inferior Alveolar Nerve in Rats

Phenotype of distinct primary sensory afferent subpopulations and caspase-3 expression following axotomy

Contact Info

Product

Resources

About