Estimation of total Terpenoids concentration in plant tissues using a monoterpene, Linalool as standard reagent.

Ghorai, Narayan; Chakraborty, Sarbani; Gucchait, Shamik; Saha, Samir K.; Biswas, Suman

doi:10.1038/protex.2012.055

Cited by 121 publications

(84 citation statements)

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“…Total terpenoids were determined according to the procedure described by Ghorai et al (2012). Dry sample (800 mg) was extracted with methanol (10 mL) for 1 h and subsequently filtered.…”

Section: Nutritional Properties and Chemical Composition Of G Lucidumentioning

confidence: 99%

The potential of Ganoderma lucidum extracts as bioactive ingredients in topical formulations, beyond its nutritional benefits

Taofiq

Heleno

Calhelha

et al. 2017

Food and Chemical Toxicology

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a b s t r a c tGanoderma lucidum was characterized in terms of nutritional value and chemical composition. Thereafter, ethanolic Soxhlet extracts were evaluated for antioxidant, anti-inflammatory, antityrosinase, antimicrobial and cytotoxic effects, and further characterized in terms of phenolic acids, polysaccharides and triterpenoids. Finally, the obtained extracts were tested as cosmeceutical ingredients. G. lucidum proved to be a source of macronutrients and important bioactive compounds such as terpenoids, specially triterpenoids, and polysaccharides. In the extracts, ganoderic acids C2, A and H were the most abundant triterpenic acids and protocatechuic, p-hydroxibenzoic and syringic acids the identified phenolics. The developed cosmeceutical formulation preserved the extract bioactivities, presented a lightyellow colour and a pH of 4.6, which is considered appropriate for cosmeceutical's design. Behind the important nutritional/bioactive composition of G. lucidum, a potential towards its valorisation in the field of cosmeceuticals is foreseeable, as deduced from the bioactivities of its ethanolic extract and preservation in the tested formulation.

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“…Total terpenoids were determined according to the procedure described by Ghorai et al (2012). Dry sample (800 mg) was extracted with methanol (10 mL) for 1 h and subsequently filtered.…”

Section: Nutritional Properties and Chemical Composition Of G Lucidumentioning

confidence: 99%

The potential of Ganoderma lucidum extracts as bioactive ingredients in topical formulations, beyond its nutritional benefits

Taofiq

Heleno

Calhelha

et al. 2017

Food and Chemical Toxicology

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“…16 Marker-based standardization of HEMIL was carried out by determining the content of lupeol and stigmasterol ( Figure 1) using a validated HPLC method described earlier. 17 Chromatographic characterization of HEMIL was also done using GC-MS/MS technique as given in appendix-supplementary material.…”

Section: Standardization Of Hemilmentioning

confidence: 99%

Standardized Extract of Mangifera Indica L. Leaves as an Antimycobacterial and Immunomodulatory Agent

Shailajan¹,

Menon²,

Kulkarni³

et al. 2016

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Background: Mangifera indica L.; the largest fruit-tree found in India, is an important component of indigenous medical systems. Mangifera indica L. leaves (MIL) have been reported to possess various biological activities and thus, can be a useful source of substances for drug development. Although the leaves have been traditionally used as antibacterial and immunomodulatory agent, there is a paucity of scientific data in support of their efficacy. The purpose of this study was an in-depth evaluation of antimycobacterial and immunomodulatory activity of a standardized extract of MIL. Material and Methods: The hexane extract of Mangifera indica L. leaves (HEMIL) was prepared and standardized. Chromatographic characterization of HEMIL was done using validated HPLC and GC-MS/MS technique. HEMIL was evaluated for antimycobacterial and immunomodulatory activity by using various in vitro and in vivo assays. Results and Discussion: HEMIL showed lupeol and stigmasterol content of 21.04 ± 0.03 mg/g and 16.99 ± 0.04 mg/g, respectively; and total terpenoids content of 112.55 ± 2.16 mg LE/g. GC-MS/MS characterization of the extract confirmed the presence of lupeol and stigmasterol and revealed five other phytochemical constituents. The safety of HEMIL was established in vitro and in vivo. HEMIL showed concentration-dependent inhibition of MTB as evident in REMA and radiorespirometry. HEMIL was also found efficacious in immunomodulatory evaluations using RAW 264.7 cells, human PBMCs, cyclophosphamide induced myelosuppressed mice and SRBCs stimulated mice. Conclusion: The promising results not only support the traditional claim of MIL as antibacterial and immunomodulatory agent but also provide data on their use in food supplements for immuno-pharmacological use.

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“…Litvak & Monson, ). Additionally, the use of methanol to extract terpenes, as per this assay method (Ghorai et al ., ), may limit the interpretation of the results of the assay because methanol is a relatively meager solvent for nonpolar terpene species.…”

Section: Discussionmentioning

confidence: 99%

“…Lastly, methanol soluble terpene levels were quantified using H 2 SO 4 as described by Ghorai et al . () against a standard curve of linalool.…”

Section: Methodsmentioning

confidence: 99%

Conifer responses to a stylet‐feeding invasive herbivore and induction with methyl jasmonate: impact on the expression of induced defences and a native folivore

Rigsby

Shoemaker

Mallinger

et al. 2019

Agri and Forest Entomology

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1 Trees attacked by multiple herbivores need to defend themselves against dynamic biotic challenges; appropriate responses to one stressor can elicit hormonal responses that are antagonistic to another. Hemlock (Tsuga canadensis) infestation by hemlock woolly adelgid (HWA; Adelges tsugae) results in the accumulation of the defensive hormone salicylic acid. 2 We explored the potential for HWA infestation to interfere with anti-folivore-induced defence signalling and its implications for a native folivore (hemlock looper; Lambdina fiscellaria). Hemlocks were infested with HWA and/or sprayed with methyl jasmonate; foliar defences were analyzed and foliage quality for looper larvae was assessed. 3 Both treatments activated foliar defensive traits, including a HWA-mediated increase in peroxidase activity and an accumulation of cell wall-bound phenolics and lignin, as well as a methyl jasmonate-mediated increase in lipoxygenase activity. The two treatments had an additive effect on other defensive traits and both treatments negatively affected looper performance. 4 These results suggest that salicylic acid and jasmonic acid are not strictly antagonistic in conifers and that both have a role in anti-folivore defence signalling. The present study illustrates the need for a better understanding of hormone signalling, cross-talk and induced responses in conifers.

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Estimation of total Terpenoids concentration in plant tissues using a monoterpene, Linalool as standard reagent.

Cited by 121 publications

References 0 publications

The potential of Ganoderma lucidum extracts as bioactive ingredients in topical formulations, beyond its nutritional benefits

The potential of Ganoderma lucidum extracts as bioactive ingredients in topical formulations, beyond its nutritional benefits

Standardized Extract of Mangifera Indica L. Leaves as an Antimycobacterial and Immunomodulatory Agent

Conifer responses to a stylet‐feeding invasive herbivore and induction with methyl jasmonate: impact on the expression of induced defences and a native folivore

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