Estimation of tissue optical parameters with hyperspectral imaging and spectral unmixing

Lu, Guolan; Qin, Xulei; Wang, Dongsheng; Chen, Zhuo Georgia; Fei, Baowei

doi:10.1117/12.2082299

Cited by 19 publications

(15 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Hyperspectral analysis has emerged as a promising diagnostic modality in recent years[5,6]. The principle of hyperspectral imaging is to acquire 2D spatial images in hundreds of contiguous bands of high-spectral resolution, including the ultraviolet, visible, and near-infrared bands.…”

Section: Introductionmentioning

confidence: 99%

Feasibility of hyperspectral analysis for discrimination of rabbit liver VX2 tumor

Duan¹,

Yuan²,

Li³

et al. 2019

WJGO

View full text Add to dashboard Cite

BACKGROUND Hepatocellular carcinoma is one of the most common malignant tumors worldwide. Currently, the most accurate diagnosis imaging modality for hepatocellular carcinoma is enhanced magnetic resonance imaging. However, it is still difficult to distinguish cirrhosis lesions, and novel diagnosis modalities are still needed. AIM To investigate the feasibility of hyperspectral analysis for discrimination of rabbit liver VX2 tumor. METHODS In this study, a rabbit liver VX2 tumor model was established. After laparotomy, under direct view, VX2 tumor tissue and normal liver tissue were subjected to hyperspectral analysis. RESULTS The spectral signature of the liver tumor was clearly distinguishable from that of the normal tissue, simply from the original spectral curves. Specifically, two absorption peaks at 600-900 nm wavelength in normal tissue disappeared but a new reflection peak appeared in the tumor. The average optical reflection at the whole waveband of 400-1800 nm in liver tumor was higher than that of the normal tissue. CONCLUSION Hyperspectral analysis can differentiate rabbit VX2 tumors. Further research will continue to perform hyperspectral imaging to obtain more information for differentiation of liver cancer from normal tissue.

show abstract

Section: Introductionmentioning

confidence: 99%

Feasibility of hyperspectral analysis for discrimination of rabbit liver VX2 tumor

Duan¹,

Yuan²,

Li³

et al. 2019

WJGO

View full text Add to dashboard Cite

show abstract

“…The images taken at 575 nm were normalized with use of the images taken at 548 nm, thereby creating an image with oxygenated Hb (oxy rel = I 575nm / I 548nm ). Consequently, according to the modified Beer-Lambert law, a relative saturation map could be computed (SO 2 rel = − log (oxy rel / oxy rel + deoxy rel ) [16]. This relative saturation map was converted into a color-based map, to make interpretation of saturation easier (blue, low saturation; red, high saturation).…”

Section: Retinal Oximetrymentioning

confidence: 99%

Retinal oximetry and fractal analysis of capillary maps in sickle cell disease patients and matched healthy volunteers

Birkhoff

Manen

Dijkstra

et al. 2019

Graefes Arch Clin Exp Ophthalmol

View full text Add to dashboard Cite

Purpose Fractal analysis can be used to quantitatively analyze the retinal microvasculature and might be a suitable method to quantify retinal capillary changes in sickle cell disease (SCD) patients. Retinal oximetry measurements might function as a proxy for the pathophysiology of cerebrovascular diseases. Moreover, hypoxia has an important role in the pathophysiology of diabetic and other retinopathies. However, little is known about the oximetry around the macula in SCD patients. With this study, we explored the feasibility to perform these quantified measurements in SCD patients. Methods Retinal microvascular and oximetry measurements were performed in eight SCD patients and eight healthy matched controls. Oximetry pictures and non-invasive capillary perfusion maps (nCPM) were obtained by the retinal function imager. Measurements were conducted twice on two different study days. Measured variables included monofractal dimension (Dbox), relative saturation, deoxygenated hemoglobin (deoxyHb), and oxygenated hemoglobin (oxyHb) concentration. Results No statistically significant differences in vessel density were found in the different annular zones (large vessels, p = 0.66; small vessels, p = 0.66) and anatomical quadrants (large vessels, p = 0.74; small vessels, p = 0.72). Furthermore, no significant between-group differences were found in the other different anatomical quadrants and annular zones around the fovea for relative saturation levels and deoxygenated Hb. However, the oxyHb levels were significantly lower in SCD patients, compared with those in matched controls in the temporal quadrants (p = 0.04; p = 0.02) and the superior nasal quadrant (p = 0.05). Conclusions Our study demonstrated the feasibility of multispectral imaging to measure retinal changes in oxygenation in both SCD patients and matched volunteers. The results suggest that in SCD patients before any structural microvascular changes in the central retina are present, functional abnormalities can be observed with abnormal oximetry measurements. Keywords Sickle cell disease. Retina analysis. Microvessels. Image processing. Computer-assisted W. A. J. Birkhoff and L. van Manen contributed equally to this work.

show abstract

“…Various hemoglobin derivatives have different typical absorption peaks at various wavelengths: the peaks for

are located at 542 nm and 577 nm, the peak for deoxyhemoglobin

are located at 555 nm, the peak for

is located at 630 nm, and the peaks for

are located at 539 and 569 nm ( Lu et al., 2015 ; Tift et al., 2014 ).…”

Section: Methodsmentioning

confidence: 99%

Assessment of carboxyhemoglobin content in the blood with high accuracy: wavelength range optimization for nonlinear curve fitting of optical spectra

Козлова

Черныш

Козлов

et al. 2020

Heliyon

View full text Add to dashboard Cite

The impact of carbon monoxide (CO) gas on the human organism is very dangerous. The affinity of CO to hemoglobin is considerably higher than that of oxygen. Thus, the interaction of CO with the blood results in a higher content of carboxyhemoglobin ( HbCO ) in red blood cells (RBCs) and correspondingly in tissue hypoxia. The disruption in the organism depends on the HbCO content in the blood. To assess any complications in the body at a given moment due to CO exposure and predict future consequences, it is necessary to measure the dynamics of hemoglobin derivative concentrations simultaneously. However, measuring HbCO and other derivatives in RBCs without hemolysis accurately is complicated due to the strong intercollinearity between the molar absorptivities of hemoglobin derivatives and superposition of absorption and scattering spectra. In the present study, to quantitatively assess the contents of the hemoglobin derivatives in the blood after exposure to CO, improved accuracy is achieved by optimizing the wavelength range used for the nonlinear curve fitting of optical spectra. Experimental spectra were measured in the wavelength range . For each experimental curve, it was established the value of optimal interval for which the correlation coefficient between experimental data and corresponding points of the theoretical fitting curve was the maximum in the wavelength range , which contains the typical absorption peaks for , , and . The concentrations obtained based on such fitting curves were considered to be highly accurate. The quantitative assessment enabled the determination of nonlinear increase with the time of CO exposure in the in vitro experiment and the study of the dynamics of hemoglobin derivative transformations during blood incubation.

show abstract

Estimation of tissue optical parameters with hyperspectral imaging and spectral unmixing

Cited by 19 publications

References 18 publications

Feasibility of hyperspectral analysis for discrimination of rabbit liver VX2 tumor

Feasibility of hyperspectral analysis for discrimination of rabbit liver VX2 tumor

Retinal oximetry and fractal analysis of capillary maps in sickle cell disease patients and matched healthy volunteers

Assessment of carboxyhemoglobin content in the blood with high accuracy: wavelength range optimization for nonlinear curve fitting of optical spectra

Contact Info

Product

Resources

About