Estimation of the Potential Antitumor Activity of Microencapsulated Lactobacillus acidophilus Yogurt Formulation in the Attenuation of Tumorigenesis in Apc(Min/+) Mice

Abstract

There is a strong correlation between orally administered probiotics and suppression of the low-grade inflammation that can lead to restoration of normal local immune functions. We studied the potential immunomodulatory and antitumorigenic properties of microencapsulated probiotic bacterial cells in a yogurt formulation in Min mice carrying a germline APC mutation. Daily oral administration of microencapsulated Lactobacillus acidophilus bacterial cells in the yogurt formulation mice resulted in significant suppression of colon tumor incidence, tumor multiplicity, and reduced tumor size. Results show that oral administration of microencapsulated L. acidophilus contributed to the stabilization of animal body weight and decreased the release of bile acids. Histopathological analyses revealed fewer adenomas in treated versus untreated animals. Furthermore, treated animals exhibited fewer gastrointestinal intra-epithelial neoplasias with a lower grade of dysplasia in detected tumors. Results suggest that oral administration of microencapsulated probiotic L. acidophilus exerts anti-tumorous activity, which consequently leads to reduced tumor outcome.

The role and potential of probiotic bacteria in the gut, and the communication between gut microflora and gut/host

“…Probiotic bacteria delivered in fermented milks or freeze dried formats reduced the incidence of diarrhea in children when compared to those receiving pasteurized yogurt; demonstrating that these delivery vehicles did not diminish probiotic function (Isolauri, Juntunen, Rautanen, Sillanaukee, & Koivula, 1991). A more recent study in a murine model of colorectal cancer demonstrated an increased positive effect of micro-encapsulated L. acidophilus delivered in yogurt compared with saline (Urbanska, Bhathena, Martoni, & Prakash, 2009). Other studies have determined the influence of the delivery matrix effect such as milk on gene expression of the probiotic (Azcarate-Peril, Tallon, & Klaenhammer, 2009).…”

Activation of Gpr109a, Receptor for Niacin and the Commensal Metabolite Butyrate, Suppresses Colonic Inflammation and Carcinogenesis

“…Multiple intestinal neoplasia (Min, Apc min/+ ) mice carry a germline-truncating mutation in one copy of Apc gene and spontaneoulsy develop adenomas throughout the intestinal tract. Lactobacillus acidophilus and certain gut microbial metabolites such as conjugated linoleic acids decrease intestinal tumorigenesis in Apc min/+ mice (Davis and Milner, 2009; Urbanska et al, 2009). In contrast, depletion of microbiota ameliorates intestinal inflammation and cancer in mouse models of spontaneous colitis ( Il10 −/− , Tbx21 −/− Rag2 −/− , or Apc min+/− ) (Garrett et al, 2009; Grivennikov et al, 2012; Li et al, 2012; Uronis et al, 2009).…”

Podem as bactérias ácido lácticas probióticas apresentarem efeito antitumoral em modelo animal de câncer de cólon? Uma revisão da literatura

“…A maioria dos animais teve o câncer de cólon induzido por DMH (Brasil, 2012, Kumar et al 2012, Zhu et al 2014 Urbanska et al (2009) verificaram que para os camundongos tratados L. acidophilus, o peso corporal manteve-se constante durante 17 dias em relação ao grupo de animais controle, minimizando a inflamação intestinal e retardando a progressão de adenomas. Kumar et al (2012) observaram que uso de L. plantarum na alimentação dos animais tratados resultou em um aumento significativo no ganho de peso em comparação ao grupo não tratado.…”