Estimation of the parameters of the Smoluchowski equation describing the occurrence of pores in a bilayer lipid membrane under soft poration

Аносов, А. А.; Smirnova, E. Yu.; Ryleeva, E. D.; Gligonov, I. A.; Korepanova, E. A.; Sharakshane, A. A.

doi:10.1140/epje/i2020-11989-0

Cited by 5 publications

(1 citation statement)

References 37 publications

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“…Ток через мембрану измеряли с помощью Ag-AgCl-электродов, подключенных к усилителю VA -10X (NPI Electronics GmbH, Тамм, Германия) с сопротивлением обратной связи 5 ГОм и временем интегрирования 20 мс. Токи через мембрану оцифровывали с помощью 16-разрядного АЦП (Е14-440, L-Card, Москва, Россия) с частотой дискретизации 1 кГц и подавали на компьютер,где проводили дальнейшую обработку сигнала[22,23]. Мембранные токи измеряли в режиме фиксации потенциала.…”

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Magnetite nanoparticles increase the conductivity of azolectin bilayer in an inhomogeneous magnetic field

Anosov,

Borisova,

Taranov

et al. 2023

JRE

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In the magnetofection method, magnetic fields and magnetic nanoparticles are used to increase the efficiency of gene delivery into cells. Magnefection enhances the introduction into cells of gene vectors with which magnetic nanoparticles are associated, due to the action of a magnetic field that holds the nanoparticles in the area of their application. It is believed that the magnetic field itself does not change the mechanism of absorption (endocytosis) of nanoparticles. Both the beneficial effect of magnetofection - delivery of the vector into the cell, and its side effect - cytotoxicity are associated with the interaction of particles with cell membranes and, in particular, with lipid bilayers. In our work, we investigated the effect of an applied stationary inhomogeneous magnetic field and spherical superparamagnetic magnetite nanoparticles with a diameter of about 4 nm on the conductivity of azolectin bilayer lipid membranes. The membranes were formed in a stationary magnetic field with a magnetic induction of up to 26 mT. The magnetic field had no effect on the conductivity of the membrane. After monitoring the membrane conductivity, magnetic nanoparticles were added to the solution surrounding the membrane. The addition was carried out on one side of the membrane so that the magnetic field attracted nanoparticles to the membrane surface. After adding nanoparticles in a magnetic field, the conductivity of the membranes increased by one to two orders of magnitude. This effect was observed for all membranes. A smooth increase in conductivity was accompanied in a number of cases (for 25% of the membranes) by the appearance of current jumps, which can be associated with the formation of through conducting pores with a radius of about 0.5 nm. The conductivity increased with increasing magnetic field gradient.

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