Estimation of the Nuclear DNA Content of Gossypium Species

Abstract

Due to unknown factors, internal standardization with H. vulgare 'Sultan' may not be appropriate for DNA content determinations of Gossypium. The current DNA content estimates support accepted cytogenetic divisions of the genus. Gossypium is a genus that exhibits genome constancy both through speciation within genomic groups and allopolyploidization.

Genome sequence of the cultivated cotton Gossypium arboreum

“…Using K-mer distribution analysis, the genome size was estimated to be 1,724 Mb ( Supplementary Fig. 3), which is consistent with a previous report 4 .…”

“…The current descendants of the two diploid cotton species that hybridized to create all cultivated allopolyploid cotton species, G. arboreum (A 2 ) and G. raimondii (D 5 ), exhibit a twofold difference in genome size 4,26 . Molecular phylogenetic analyses 27 suggested a divergence time for G. arboreum and G. raimondii of 2-13 million years ago, with their common ancestor having diverged from T. cacao 18-58 million years ago (Fig.…”

Repeated polyploidization of Gossypium genomes and the evolution of spinnable cotton fibres

“…The assembly size is near the centre of genome-size estimates of 880 Mb from flow cytometry 37 , 630 Mb from Feulgen cytophotometry 38 , and 650 Mb 39 and 770 Mb 40 from re-naturation kinetics.…”

Toward Sequencing Cotton (Gossypium) Genomes: Figure 1.

“…The haploid genome sizes are estimated to be approximately 880 Mb for G. raimondii ÔUlbrichÕ, approximately 1.75 Gb for G. arboreum, and approximately 2.5 Gb for G. hirsutum (Hendrix and Stewart, 2005). Variation in DNA content in the diploid species reflects increases and decreases in copy numbers of various repeat families (Zhao et al, 1998), especially retrotransposon-like elements (Hawkins et al, 2006).…”