Estimation of the Expanded Uncertainty of an Analytical Method to Quantify Aluminum in Tissue of Sprague Dawley Rats by FAAS and ETAAS

García-Alegría, Alejandro Monserrat; Gómez-Álvarez, Agustín; Anduro-Corona, Iván; Burgos‐Hernández, Armando; Ruíz-Bustos, Eduardo; Canett-Romero, Rafael; Cáñez-Carrasco, María Guadalupe; Astiazarán-García, Humberto

doi:10.1007/s12647-017-0203-y

Cited by 4 publications

(1 citation statement)

References 25 publications

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“…The determination of aluminum concentration in the standard, certified reference material, and rat breast tissue was performed using atomic absorption spectroscopy with an AAnalyst 400 automated equipment in a graphite furnace absorption atomic spectroscopy (GFAAS) or in the electrothermal absorption atomic spectroscopy (ETAAS) mode, under the operating conditions recommended by the manufacturer [40,41]. The analytical method was previously optimized and its expanded uncertainty for quantifying aluminum by FAAS (flame absorption atomic spectroscopy) and ETAAS was subsequently validated and estimated uncertainty [42][43][44].…”

Section: Determination Of Aluminum Concentration In Breast Tissuesmentioning

confidence: 99%

Genotoxic Effects of Aluminum Chloride and Their Relationship with N-Nitroso-N-Methylurea (NMU)-Induced Breast Cancer in Sprague Dawley Rats

García-Alegría

Gómez-Álvarez

Anduro-Corona

et al. 2020

Toxics

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Recently, soluble forms of aluminum for human use or consumption have been determined to be potentially toxic due to their association with hepatic, neurological, hematological, neoplastic, and bone conditions. This study aims to assess the genotoxic effect of aluminum chloride on genomic instability associated with the onset of N-nitroso-N-methylurea (NMU)-induced breast cancer in Sprague Dawley rats. The dietary behavior of the rats was assessed, and the concentration of aluminum in the mammary glands was determined using atomic absorption spectroscopy. Genomic instability was determined in the histological sections of mammary glands stained with hematoxylin and eosin. Moreover, micronucleus in peripheral blood and comet assays were performed. The results of dietary behavior evaluation indicated no significant differences between the experimental treatments. However, aluminum concentration in breast tissues was high in the +2000Al/−NMU treatment. This experimental treatment caused moderate intraductal cell proliferation, lymph node hyperplasia, and serous gland adenoma. Furthermore, micronucleus and comet test results revealed that +2000Al/−NMU led to a genotoxic effect after a 10-day exposure and the damage was more evident after a 15-day exposure. Therefore, in conclusion, genomic instability is present and the experimental conditions assessed are not associated with breast cancer.

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Section: Determination Of Aluminum Concentration In Breast Tissuesmentioning

confidence: 99%

Genotoxic Effects of Aluminum Chloride and Their Relationship with N-Nitroso-N-Methylurea (NMU)-Induced Breast Cancer in Sprague Dawley Rats

García-Alegría

Gómez-Álvarez

Anduro-Corona

et al. 2020

Toxics

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Flame and Graphite Furnace Atomic Absorption Spectrometry in Environmental Analysis

Sperling

2022

Encyclopedia of Analytical Chemistry

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Atomic absorption spectrometry (AAS) is a well‐established technique for the quantitative determination of elements in environmental materials at trace and ultratrace levels. AAS is an optical atomic spectrometric technique based on the measurement of the specific absorption originated from the free, unionized atoms in the gas phase. For transferring the analyte to free atoms, different types of atomizers are in use, out of which the flame and the graphite furnace are the most often used atomizers. Typical detection limits of flame AAS (FAAS) are of the order of 1–100 μg L −1 , making it a perfect tool for the determination of minor and trace elements, at least for contaminated samples. Graphite furnace AAS (GFAAS), offering detection limits which are about a factor of 20–200 lower than for FAAS, is the standard method for many trace elements especially for background values, and for unpolluted samples, such as freshwater and biological materials. AAS in its conventional configuration is a single element technique, which has to be used in a sequential mode when more than one element has to be determined. However, there are commercial instruments available, that can be used for the determination of six to eight elements simultaneously. While most manufacturers provide instruments working with line sources, one manufacturer has commercialized high‐resolution instruments working with continuum sources (HRCS‐AAS).

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Estimation of Uncertainty in the Determination of Serum Electrolytes (Na, K, Ca, Mg) by Flame Atomic Absorption Spectroscopy

García-Alegría

Cáñez-Carrasco

Serna-Félix³

et al. 2018

MAPAN

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Estimation of the Expanded Uncertainty of an Analytical Method to Quantify Aluminum in Tissue of Sprague Dawley Rats by FAAS and ETAAS

Cited by 4 publications

References 25 publications

Genotoxic Effects of Aluminum Chloride and Their Relationship with N-Nitroso-N-Methylurea (NMU)-Induced Breast Cancer in Sprague Dawley Rats

Genotoxic Effects of Aluminum Chloride and Their Relationship with N-Nitroso-N-Methylurea (NMU)-Induced Breast Cancer in Sprague Dawley Rats

Flame and Graphite Furnace Atomic Absorption Spectrometry in Environmental Analysis

Estimation of Uncertainty in the Determination of Serum Electrolytes (Na, K, Ca, Mg) by Flame Atomic Absorption Spectroscopy

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