Estimation of salt tolerance in Andrographis paniculata accessions using multiple regression model

Abstract

The complexity and polygenic nature of the salt tolerance trait in plants needs to develop a multiple indicator in the screening process. The mentioned issue led us to carry out an experiment to identify tolerant genotypes through multiple parameters in Andrographis paniculata. For this purpose, the 40-days seedlings were grown in different salinity levels (control, 4, 8, 12 and 16 dS m−1) on Hoagland's medium. The results indicated that salinity had a significant effect on the morphological, physiological and biochemical traits. All measured morphological traits, and chlorophyll, K+ and Ca2+ content were significantly decreased with increasing salinity levels, while proline and Na+ content increased. The present exploration revealed that, salt tolerance index (STI), using the multiple regression model, demonstrated a more stable trend than the single variable assay (total dry weight). Furthermore, STI based on multiple regression analysis gives an accurate definition of salttolerant individuals. Under salt stress, tolerant accessions had high STI and produced higher proline, K+ and Ca2+, and lower Na+ content than sensitive accessions. Cluster analysis based on related traits to STI, indicated high similarity in each group. These outcomes can be utilized to evaluate the salt tolerance threshold in the species and may have a great advantage over conventional methods. Probably, our upshots can be applied in the next breeding programs to develop salt-tolerant varieties.

A Classical Genetic Solution to Enhance the Biosynthesis of Anticancer Phytochemicals in Andrographis paniculata Nees

“…A similar report has been released about the morphological characteristics involved in salt tolerance in AP [66]. Regardless of the non-diallelic methods, the recorded heritabilities could be interpreted as being compatible outcomes with our present results, suggesting that despite all the difficulties associated with, AP has a high potential to be subjected to the intraspecific hybridization or outcrossing [23], [27].…”

Physiology of salinity tolerance in Aegilops cylindrica

“…In the present study, STI could discriminate between the two group of genotypes that are adapted and thrive under the adverse conditions as well as normal conditions, whilst failed to distinguish the most salinity-tolerant genotypes. This result differs from that of a recent study in Andrographis paniculata, suggesting STI as a discriminator between salt-tolerant and salt-sensitive accessions at the seedling stage (Talei et al 2013). However, this index may be more applicable to a variety of environment with fluctuating stress conditions such as drought which depends on annual climatic variables (precipitation, temperature and atmospheric CO 2 ) (Golabadi et al 2006).…”

Salt stress-induced protein pattern associated with photosynthetic parameters and andrographolide content in Andrographis paniculata Nees

“…25) Heritability of morphological and phytochemical characteristics of A. paniculat had been under scrutiny in very recent studies. 25) Two of these researches have been conducted under salinity condition, and the common point of these researches with the present study was recording high broad-sense heritabilities for proline, TDW, total chlorophyll, 8) as well as for AG. 24) As a remarkable evaluation, comprehensive genetic analyses of andrographolides in A. paniculata using diallel technique signified moderate broad-sense heritability for AG, while the narrow-sense heritability was suffering from a negative value, under normal condition.…”