Estimation of Salivary Cortisol in Children with Rampant Caries

Rai, Kavita; Hegde, Amitha M; Shetty, Sowmya; Shetty, Sanath Kumar

doi:10.17796/jcpd.34.3.l858480k80031jn2

Cited by 18 publications

(19 citation statements)

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“…When secreted a higher levels for longer periods of time, it relates to a state of chronic stress. Salivary cortisol levels are found to be elevated in children with rampant caries and those levels will decrease after restorative treatment is provided [25]. Furthermore, children with early childhood caries have significantly higher levels of salivary cortisol when compared to caries free children, and a positive correlation of salivary cortisol levels of the mothers of children affected by early childhood caries exist [26], suggesting both child and mother salivary cortisol level may impact incidence of early childhood caries.…”

Section: Discussionmentioning

confidence: 99%

Genetic mapping of high caries experience on human chromosome 13

Küchler

Deeley

et al. 2013

BMC Med Genet

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BackgroundOur previous genome-wide linkage scan mapped five loci for caries experience. The purpose of this study was to fine map one of these loci, the locus 13q31.1, in order to identify genetic contributors to caries.MethodsSeventy-two pedigrees from the Philippines were studied. Caries experience was recorded and DNA was extracted from blood samples obtained from all subjects. Sixty-one single nucleotide polymorphisms (SNPs) in 13q31.1 were genotyped. Association between caries experience and alleles was tested. We also studied 1,481 DNA samples obtained from saliva of subjects from the USA, 918 children from Brazil, and 275 children from Turkey, in order to follow up the results found in the Filipino families. We used the AliBaba2.1 software to determine if the nucleotide changes of the associated SNPs changed the prediction of the presence of transcription-binding site sequences and we also analyzed the gene expression of the genes selected based on binding predictions. Mutation analysis was also performed in 33 Filipino individuals of a segment of 13q31.1 that is highly conserved in mammals.ResultsStatistically significant association with high caries experience was found for 11 markers in 13q31.1 in the Filipino families. Haplotype analysis also confirmed these results. In the populations used for follow-up purposes, associations were found between high caries experience and a subset of these markers. Regarding the prediction of the transcription-binding site, the base change of the SNP rs17074565 was found to change the predicted-binding of genes that could be involved in the pathogenesis of caries. When the sequence has the allele C of rs17074565, the potential transcription factors binding the sequence are GR and GATA1. When the subject carries the G allele of rs17074565, the potential transcription factor predicted to bind to the sequence is GATA3. The expression of GR in whole saliva was higher in individuals with low caries experience when compared to individuals with high caries experience (p = 0.046). No mutations were found in the highly conserved sequence.ConclusionsGenetic factors contributing to caries experience may exist in 13q31.1. The rs17074565 is located in an intergenic region and is predicted to disrupt the binding sites of two different transcription factors that might be involved with caries experience. GR expression in saliva may be a biomarker for caries risk and should be further explored.

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Section: Discussionmentioning

confidence: 99%

Genetic mapping of high caries experience on human chromosome 13

Küchler

Deeley

et al. 2013

BMC Med Genet

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“…Furthermore, morning salivary cortisol concentration was lower three months after caries treatment but still higher than for caries-free children (Pani and Al Odhaib 2013). Similar result has also been reported in 5-10-year-old children (Rai, Hegde et al 2010). Equivalent results, association between caries and salivary cortisol concentrations have been described in 5-year-old children by Boyce et al (2010).…”

Section: Stress Response and Dental Cariessupporting

confidence: 78%

Children with orofacial clefts : dental caries and health-related quality of life

Sundell¹

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“…Una nutrición inadecuada durante la infancia puede tener efectos adversos en el comportamiento, desarrollo cognitivo y el desempeño escolar de los niños, y afectar a su habilidad para concentrarse y desarrollar tareas complejas 21 . b) Caries-estrés-desarrollo: se ha reportado que el nivel de cortizol salival en niños con caries rampante disminuye tras recibir tratamiento dental 22 . Igualmente, el dolor que genera la caries avanzada actúa como estresor, lo que aumentará la secreción de cortizol.…”

Section: Discussionunclassified