Estimation of plasma haemoglobin by a modified kinetic method using o-tolidine

Goyal, Madhur M.; Basak, Anjan

doi:10.1007/s12291-009-0006-1

Cited by 13 publications

(13 citation statements)

References 11 publications

(3 reference statements)

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“…Free hemoglobin, haptoglobin‐hemoglobin complexes, hemopexin‐heme complexes, and methemalbumin are separated by alkaline electrophoresis. A heme‐specific stain, o‐Tolidene, and hydrogen peroxide allow visualization of the heme‐containing components . Several heme‐specific stains are available, but o‐Tolidene was chosen due to its high sensitivity .…”

Section: Methods Of Haptoglobin Testingmentioning

confidence: 99%

Haptoglobin testing in hemolysis: Measurement and interpretation

2014

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Haptoglobin is primarily produced in the liver and is functionally important for binding free hemoglobin from lysed red cells in vivo, preventing its toxic effects. Because haptoglobin levels become depleted in the presence of large amounts of free hemoglobin, decreased haptoglobin is a marker of hemolysis. Despite its ubiquity and importance, a paucity of literature makes testing difficult to interpret. This review highlights the many physiological roles that have been recently elucidated in the literature. Different methodologies have been developed for testing, including spectrophotometry, immunoreactive methods, and gel electrophoresis. These are covered along with their respective advantages and disadvantages. As there is no single gold standard for hemolysis, validation studies must rely on a combination of factors, which are reviewed in this article. Pitfalls and limitations of testing are also addressed. False positives can occur in improper specimen preparations, cirrhosis, elevated estrogen states, and hemodilution. False negatives can occur in hypersplenism and medications such as androgens and corticosteroids. Haptoglobin testing in the setting of inflammation is additionally discussed as interpretation can be difficult in this setting. Given the widespread use of haptoglobin testing, it is vital that clinicians and laboratory staff understand the principles and correct interpretation of this test.

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Section: Methods Of Haptoglobin Testingmentioning

confidence: 99%

Haptoglobin testing in hemolysis: Measurement and interpretation

2014

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“…Haemoglobin was evaluated using a modified kinetic technique of Goyal and Basak (2009) in which haem acts as a chemical catalyst to break down hydrogen peroxide into water and nascent…”

Section: Evaluation Of Haemoglobinmentioning

confidence: 99%

“…Nascent oxygen then oxidizes o-tolidine to give an oxidised product of green-blue colour. The rate of colour development is directly proportional to haem concentration (Goyal & Basak, 2009). O-tolidine stock solution was prepared by dissolving 2 g of o-tolidine in 100 mL of solvent (20 mL of glacial acetic acid and 80 mL of ethanol) to create a stock solution.…”

Section: Evaluation Of Haemoglobinmentioning

confidence: 99%

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A comparison of bleeding efficiency, microbiological quality and lipid oxidation in goats subjected to conscious halal slaughter and slaughter following minimal anesthesia

et al. 2015

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The study assessed the effect of conscious halal slaughter and slaughter following minimal anesthesia on bleeding efficiency of goats and keeping quality of goat meat. Ten Boer cross bucks were divided into two groups and subjected to either halal slaughter without stunning (HS) or minimal anesthesia prior to slaughter (AS). The blood lost during exsanguination was measured. Residual blood was further quantified by determination of hemoglobin and myoglobin content in longissimus lumborum muscle. Storage stability of the meat was evaluated by microbiological analysis and lipid oxidation. Blood loss at exsanguination, residual hemoglobin and lipid oxidation were not significantly different (p>0.05) between HS and AS. Lactic acid bacteria was the only microbe that was significantly elevated after 24h of storage at 4°C in the AS group. In conclusion, slaughtering goats under minimal anesthesia or fully conscious did not affect bleeding efficiency and keeping quality of goat meat.

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“…The colorimetric assay involves a Hgb catalyzed redox reaction between 3,3′-Dimethyl-[1,1′biphenyl]-4,4′-diamine (o-tolidine) and hydrogen peroxide yielding greenish-blue colored oxidized o-tolidine products (23). This reaction is mechanistically similar to the well-established and commercially adopted chemical assay which has been utilized in the spectrophotometric detection of Hgb in plasma, criminal inspection, and in portable POC photometers for anemia diagnosis (19,24,25).…”

Section: Resultsmentioning

confidence: 99%

Anemia Diagnosis on a Simple Paper-based Assay

Bandyopadhyay

Kar

et al. 2018

Preprint

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In developing countries, the maternal and neonatal mortality rate is often affected by prenatal period anemia, a preventable and ubiquitous impairment attributed due to low hemoglobin (Hgb) concentration. We report the development of a simple, frugal (~ 0.02 $ per test), rapid and high fidelity paper-based colorimetric microfluidic device for point-of-care (POC) detection of anemia. We validate our findings with 32 blood samples collected from different patients covering a wide spectrum of anemia and subsequently, compare with standard pathological results measured using a hematology analyzer. POC based Hgb estimates are correlated with the pathological gold standard estimates of Hgb levels (r = 0.909), and the POC test method yielded similar sensitivity and specificity for detecting mild anemia (n = 8) (<11 g/dl) (sensitivity: 87.5 %, specificity: 100 %) and for severe anemia (n = 3) (<7 g/dl) (sensitivity: 100 %, specificity: 100 %). The estimated Hgb levels are, within 1.5 g/dl from the pathological estimate, for 91 % of the blood samples. Results demonstrate the elevated efficacy and viability of this POC colorimetric diagnostic test, in comparison to the state-of-the-art complex and expensive diagnostic tests for anemia detection.SC conceptualized and envisioned this study. SKB and SB were responsible for the design, fabrication, experiments, post-processing, and analysis. SK was involved in technical design and strategy for execution of the study with SKB and SB. NKS actively involved in the clinical validation part of the work. All members were involved in technical discussion in different stages of the project and contributed to manuscript writing.

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Estimation of plasma haemoglobin by a modified kinetic method using o-tolidine

Abstract: ABSTRACT

Cited by 13 publications

References 11 publications

Haptoglobin testing in hemolysis: Measurement and interpretation

Haptoglobin testing in hemolysis: Measurement and interpretation

A comparison of bleeding efficiency, microbiological quality and lipid oxidation in goats subjected to conscious halal slaughter and slaughter following minimal anesthesia

Anemia Diagnosis on a Simple Paper-based Assay

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