Estimation of pipemidic acid and N-nitrosopipemidicacid by high-performance liquid chromatography

Zio, Cesare; Massarani, Gabriele; Pietta, Pergiorgio

doi:10.1016/s0021-9673(00)84942-0

Cited by 8 publications

(1 citation statement)

References 9 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Blood and urine were collected from a normal male volunteer after oral administration rosoxacin (4) and pipemidic acid (6). We observed that MK-366 could not be eliited from Spherisorb (silica), LiChrosorb NH2, Vydac cation exchange, and Spherisorb ODS columns (Applied Science Laboratories, State College, Pa.) under a variety of mobile phase conditions.…”

Section: Norfloxacin (Mk-366) [1-ethyl-6-fluoro-14-dihydro-4-oxo-7-(mentioning

confidence: 99%

Determination of norfloxacin, a new nalidixic acid analog, in human serum and urine by high-performance liquid chromatography

Boppana¹,

Swanson²

1982

Antimicrob Agents Chemother

View full text Add to dashboard Cite

A high-performance liquid chromatographic method for the analysis of norfloxacin [1-ethyl-6-fluoro-1,4-dihydro-4-oxo-7-(1-piperazinyl)-3-quinolinecarboxylic acid], a new nalidixic acid analog, in human serum and urine is described. A statistical evaluation of the assay data showed acceptable accuracy and precision for 0.1 to 10.0 ,ug of MK-366 per ml of serum and for 1.0 to 500 ,ug of MK-366 per ml of urine. MK-366 was extracted from serum and urine at pH 7.5 with methylene chloride and back-extracted with sodium hydroxide solution. Chromatography was performed on an anion-exchange column with acetonitrilephosphate buffer as the mobile phase; UV absorbance was monitored at 273 nm. The method was used to measure MK-366 in clinical specimens.boxylic acid] is a new broad-spectrum antibacterial agent that is structurally related to nalidixic acid (Fig. 1). MK-366 exhibits greater antibacterial activity against both gram-positive and gram-negative bacteria than other nalidixic acid analogs and exhibits greater activity against Pseudomonas aeruginosa than gentamicin (1,2 Extraction of samples. Serum (1 ml) or urine (200 pul) was thoroughly mixed with 100 p.l of 0.05 N NaOH (containing standards preparing the standard curve) in a 40-ml glass extraction tube. Methylene chloride (11 ml) and 0.5 M sodium phosphate buffer (0.5 ml, pH 7.5) were then added, and the tubes were gently shaken for 10 min on a mechanical shaker. After phase separation by centrifugation at 1,500 x g for 5 min, 10 ml of the methylene chloride layer was transferred to a second 40-ml extraction tube. Fresh methylene chloride (11 ml) was added to the first extraction tube, and a second extraction was performed; 10 ml of the second extract was pooled with the first organic phase.

show abstract

Section: Norfloxacin (Mk-366) [1-ethyl-6-fluoro-14-dihydro-4-oxo-7-(mentioning

confidence: 99%

Determination of norfloxacin, a new nalidixic acid analog, in human serum and urine by high-performance liquid chromatography

Boppana¹,

Swanson²

1982

Antimicrob Agents Chemother

View full text Add to dashboard Cite

show abstract

Gas‐Liquid Chromatographic Determination of Pipemidic Acid

Chen

Lin

1983

J Chinese Chemical Soc

View full text Add to dashboard Cite

A gas chromatographic method was established for the quantitative analysis of pipemidic acid. The method is based on alkylation of pipemidic acid with diazomethane catalyzed by boron trifluoride. The sample was chromatographed on a stainless steel column packed with 5% OV‐101 on Chromosorb G‐HP and n‐octacosane was used as an internal standard. Quantitation is achieved by measuring peak‐height ratio. The method was satisfactorily applied to the analysis of pipemidic acid in tablet.

show abstract

Simple method for the determination of pipemidic acid in biological fluids by high-performance liquid chromatography

Fukuhara

Matsuki

1987

Journal of Chromatography B: Biomedical Sciences and Applicatio

View full text Add to dashboard Cite

Estimation of pipemidic acid and N-nitrosopipemidicacid by high-performance liquid chromatography

Cited by 8 publications

References 9 publications

Determination of norfloxacin, a new nalidixic acid analog, in human serum and urine by high-performance liquid chromatography

Determination of norfloxacin, a new nalidixic acid analog, in human serum and urine by high-performance liquid chromatography

Gas‐Liquid Chromatographic Determination of Pipemidic Acid

Simple method for the determination of pipemidic acid in biological fluids by high-performance liquid chromatography

Contact Info

Product

Resources

About