Estimation of Phytin Phosphorus

Harris, Robert S.; Mosher, L. Malcolm

doi:10.1021/ac50091a007

Cited by 15 publications

(1 citation statement)

References 4 publications

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“…Phytate forms white precipitate with Fe 3+ ions, while the titration endpoint is observed by the appearance of red-colored iron(III) thiocyanate complex. To overcome difficulties with the endpoint recognition, improvements of the original method were introduced in the following years by Rather [ 34 ] (1917), Averill and King [ 35 ] (1926), and Harris and Mosher [ 36 ] (1934) implementing modifications into direction of total phosphorous assay and/or quantification of precipitated iron. As an example of an early classical analysis of phytic acid from this period is worth mentioning the publication of McCance and Widdowson [ 37 ] (1935) who used the approach based on the extraction of dried food samples with HCl, filtration, and neutralization with NaOH to prepare the sample solution which was precipitated with FeCl 3 .…”

Section: Classical Analytical Methodsmentioning

confidence: 99%

Analytical Methods for Determination of Phytic Acid and Other Inositol Phosphates: A Review

Marolt

Kolar

2020

Molecules

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From the early precipitation-based techniques, introduced more than a century ago, to the latest development of enzymatic bio- and nano-sensor applications, the analysis of phytic acid and/or other inositol phosphates has never been a straightforward analytical task. Due to the biomedical importance, such as antinutritional, antioxidant and anticancer effects, several types of methodologies were investigated over the years to develop a reliable determination of these intriguing analytes in many types of biological samples; from various foodstuffs to living cell organisms. The main aim of the present work was to critically overview the development of the most relevant analytical principles, separation and detection methods that have been applied in order to overcome the difficulties with specific chemical properties of inositol phosphates, their interferences, absence of characteristic signal (e.g., absorbance), and strong binding interactions with (multivalent) metals and other biological molecules present in the sample matrix. A systematical and chronological review of the applied methodology and the detection system is given, ranging from the very beginnings of the classical gravimetric and titrimetric analysis, through the potentiometric titrations, chromatographic and electrophoretic separation techniques, to the use of spectroscopic methods and of the recently reported fluorescence and voltammetric bio- and nano-sensors.

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Section: Classical Analytical Methodsmentioning

confidence: 99%

Analytical Methods for Determination of Phytic Acid and Other Inositol Phosphates: A Review

Marolt

Kolar

2020

Molecules

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Effect of processing on the physico‐chemical properties of African yambean, Sphenostylis stenocarpa (Hochst ex A. Rich) Harms

Agunbiade

Longe

1996

Nahrung

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be helpful in ameliorating the prevalent mineral deficiencies and would lead to a better mineral status of people in developing countries. Summary a substantial loss of Ca. A phytic acid loss by 4.5 to 36.7% was obtained by soaking and heat treatment of yambeans. Trypsin inhibitor activity (TIA), although decreased substantially with cooking, slightly increased during the pre-soaking step. Alkali and alkali-brine soaked seed flours, with a wateroil-absorption index of about 2.0, exhibited the best hydrophilic-lipophilic balance.Soaked and heat-treated yambean seeds were studied for some of their chemical and physical characteristics. An increase of between 2.9 and 29.4% in the ash contents reflected mineral pick-up by seeds pre-soaked in salt solutions. Subsequently, remarkable losses of Ca, K, and Na resulted when seeds pre-soaked in salt solutions were cooked.

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Studies on dried peas. I. —The determination of phytate phosphorus

Holt

1955

J Sci Food Agric

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The relation of phytate concentration to colour produced by standard quantities of thiocyanate and ferric iron in a solution is inverse and almost linear. This is the basis of the method proposed for the determination of phytate phosphorus. It has been applied to the determination of quantities between 75 and 150 ug. in extracts from dried peas with an accuracy of ± 5%. Chloride, sulphate, citrate, tartrate and malate do not interfere in concentrations of the same order as that of the phytate. Interference by o‐I mg. of orthophosphate phosphorus is negligible; 0.4 mg. causes a positive error of less than 5%. It is suggested that there may be less interference in aqueous alcohol.

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Estimation of Phytin Phosphorus

Cited by 15 publications

References 4 publications

Analytical Methods for Determination of Phytic Acid and Other Inositol Phosphates: A Review

Analytical Methods for Determination of Phytic Acid and Other Inositol Phosphates: A Review

Effect of processing on the physico‐chemical properties of African yambean, Sphenostylis stenocarpa (Hochst ex A. Rich) Harms

Studies on dried peas. I. —The determination of phytate phosphorus

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