Estimation of Outcrossing Rate in Common Bean

Abstract

To assess genetic and environmental variation for outcrossing in common bean (Phaseolus vulgaris L.), a bulk of nine purplehypocotyled (dominant), black-seeded, common bean lines (pollen parents) and six green-hypocotyled (recessive), white-seeded, common bean lines (seed parents) representing Mexican germplasm were used to estimate outcrossing rate. The experiment was conducted at Irvine and Riverside, CA, in mid-May and mid-July of 1989 and 1991. Outcrossing estimates were based on the proportion of purplehypocotyled progeny from green-hypocotyled parents. Approximately 1400 single-plant families were progeny tested in which 120 278 seedlings were scored for the presence of anthocyanin in the hypocotyl. Of these, 113 246 were classified as selfed progeny and 7031 as hybrid progeny, giving rise to a weighted mean outcrossing rate of 6.9%. Entire progenies from each maternal line were grown and scored, and the minimum and maximum rates of outcrossing observed ranged between 0.0 and 78.0%. Mean outcrossing rate for the six white-seeded lines ranged from 4.41 to 10.16%. However, differences in outcrossing rate among these lines were not significant, nor were differences between dates of planting, locations, or years. The combined analysis of variance showed significant location x planting date x line interaction and year x location x line interaction, indicating that environmental factors had a strong effect on rate of outcrossing in the lines examined. K NOWLEDGE about the breeding system of a crop is important to design effective breeding schemes to improve that crop. Common bean was listed as a predomi

Hybridization rates between lettuce (Lactuca sativa) and its wild relative (L. serriola) under field conditions

“…Moreover, recent studies have shown that low outcrossing rates do not prevent cultivated genes from moving in sympatric wild populations. These studies include both, autogamous and/or predominantly insect-pollinated species like soybean, bean or cotton (Ibarra Perez et al, 1997;Nakamaya and Yamaguchi, 2002;Van Deynze et al, 2005), as well as windpollinated autogamous species as rice or foxtail millet (Chen et al, 2004;Song et al, 2003;Wang et al, 1997;Zhang et al, 2003).…”

Linkage disequilibrium based association mapping of micronutrients in common bean (Phaseolus vulgaris L.): a collection of Jammu & Kashmir, India

“…It is cultivated in many parts of world as dry beans and green beans (Ibarra-Perez et al 1997;Burle et al 2010). The sufficient amount of proteins (20-28%), carbohydrates, vitamins, dietary fiber (56%), micronutrients e.g., iron (Fe) (70 mg/ kg) and zinc (Zn) (33 mg/kg), vitamin B complex (niacin, riboflavin, folic acid and thiamine) and polyunsaturated fatty acids present in common bean have made it a potential staple food to overcome the problem of hunger (Pennington and Young 1990;Welch et al 2000;Broughton et al 2003;Guzman and Infante 2007).…”

Performance evaluation of common bean (Phaseolus vulgaris L.) varieties in Raya Valley, Northern Ethiopia

“…There are about 50 species under the genus Phaseolus. Phaseolus vulgaris L. was follow-on from wild ancestors distributed from Northern Mexico to Northwestern Argentina (Ibarra-Perez et al, 1997;Debouck, 1999). The ancestors of P. vulgaris L. become visible to have created two distinctive gene pools one in Mesoamerica (Mexico and Central America) and the other in the Southern Andes, a mountain range in South America (Burle et al, 2010).…”