Estimation of nuclear genome size of the genus Mycetophylax Emery, 1913: Evidence of no whole-genome duplication in Neoattini

Cardoso, Danon Clemes; Carvalho, Carlos Roberto; Cristiano, Maykon Passos; Soares, Fernanda Aparecida Ferrari; Tavares, Mara Garcia

doi:10.1016/j.crvi.2012.09.012

Cited by 9 publications

(18 citation statements)

References 29 publications

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“…Analysis of wingless and long–wave rhodopsin led to the recent molecular phylogenetic hypothesis of the Mycetophylax genus [17], which was in agreement with the morphological features. Recently, the nuclear content of the three species were estimated by flow cytometry, data that provided noteworthy information at a higher level than the species level [14], but information concerning the Mycetophylax karyotype is no longer available.…”

Section: Introductionmentioning

confidence: 99%

The Role of Fusion in Ant Chromosome Evolution: Insights from Cytogenetic Analysis Using a Molecular Phylogenetic Approach in the Genus Mycetophylax

et al. 2014

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Among insect taxa, ants exhibit one of the most variable chromosome numbers ranging from n = 1 to n = 60. This high karyotype diversity is suggested to be correlated to ants diversification. The karyotype evolution of ants is usually understood in terms of Robertsonian rearrangements towards an increase in chromosome numbers. The ant genus Mycetophylax is a small monogynous basal Attini ant (Formicidae: Myrmicinae), endemic to sand dunes along the Brazilian coastlines. A recent taxonomic revision validates three species, Mycetophylax morschi, M. conformis and M. simplex. In this paper, we cytogenetically characterized all species that belongs to the genus and analyzed the karyotypic evolution of Mycetophylax in the context of a molecular phylogeny and ancestral character state reconstruction. M. morschi showed a polymorphic number of chromosomes, with colonies showing 2n = 26 and 2n = 30 chromosomes. M. conformis presented a diploid chromosome number of 30 chromosomes, while M. simplex showed 36 chromosomes. The probabilistic models suggest that the ancestral haploid chromosome number of Mycetophylax was 17 (Likelihood framework) or 18 (Bayesian framework). The analysis also suggested that fusions were responsible for the evolutionary reduction in chromosome numbers of M. conformis and M. morschi karyotypes whereas fission may determines the M. simplex karyotype. These results obtained show the importance of fusions in chromosome changes towards a chromosome number reduction in Formicidae and how a phylogenetic background can be used to reconstruct hypotheses about chromosomes evolution.

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Section: Introductionmentioning

confidence: 99%

The Role of Fusion in Ant Chromosome Evolution: Insights from Cytogenetic Analysis Using a Molecular Phylogenetic Approach in the Genus Mycetophylax

et al. 2014

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“…Such information is also important to clarify the relationship between variation in genome size and chromosome number (Tsutsui et al 2008, Cardoso et al 2012, Jacobson et al 2012), and direct the selection of species for genome sequencing projects (Hardie et al 2002, Gregory 2005, Geraci et al 2007). …”

Section: Introductionmentioning

confidence: 99%

Comparative cytogenetics and derived phylogenic relationship among Sitophilus grain weevils (Coleoptera, Curculionidae, Dryophthorinae)

Silva

Braga

Corrêa

et al. 2018

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Cytogenetic characteristics and genome size are powerful tools for species characterization and identification of cryptic species, providing critical insights into phylogenetic and evolutionary relationships. Sitophilus Linnaeus, 1758 grain weevils can benefit from such tools as key pest species of stored products and also as sources of archeological information on human history and past urban environments. Moreover, the phylogenetic relationship among these weevil species remains controversial and is largely based on single DNA fragment analyses. Therefore, cytogenetic analyses and genome size determinations were performed for four Sitophilus grain weevil species, namely the granary weevil Sitophilus granarius (Linnaeus, 1758), the tamarind weevil S. linearis (Herbst, 1797), the rice weevil S. oryzae (Linnaeus, 1763), and the maize weevil S. zeamais Motschulsky, 1855. Both maize and rice weevils exhibited the same chromosome number (2n=22; 10 A + Xyp). In contrast, the granary and tamarind weevils exhibited higher chromosome number (2n=24; 11 A + Xyp and 11 A + neo-XY, respectively). The nuclear DNA content of these species was not proportionally related to either chromosome number or heterochromatin amount. Maize and rice weevils exhibited similar and larger genome sizes (0.730±0.003 pg and 0.786±0.003 pg, respectively), followed by the granary weevil (0.553±0.003 pg), and the tamarind weevil (0.440±0.001 pg). Parsimony phylogenetic analysis of the insect karyotypes indicate that S. zeamais and S. oryzae were phylogenetically closer than S. granarius and S. linearis, which were more closely related and share a more recent ancestral relationship.

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“…Only Mycetophylax simplex differed from their congeneric species, which may be due to the difference in the heterochromatin content (Cardoso et al, 2012). Although M. morschi shows variation in the chromosome number, differences in the DNA content within its variants were not observed (Cardoso et al, 2012). The stingless bees with high heterochromatin content Melipona rufiventris and Melipona mondury showed the same chromosome number, with little difference in the genome size; this observation suggests difference in the amount of heterochromatin (Lopes et al, 2009).…”

Section: Discussionmentioning

confidence: 92%

“…The fungus-growing ants, Mycetophylax morschi and Mycetophylax conformis, showed the same genome size and chromosome number. Only Mycetophylax simplex differed from their congeneric species, which may be due to the difference in the heterochromatin content (Cardoso et al, 2012). Although M. morschi shows variation in the chromosome number, differences in the DNA content within its variants were not observed (Cardoso et al, 2012).…”

Section: Discussionmentioning

confidence: 99%

“…Data concerning the genome size in Formicidae are restricted to 73 species (Tsutsui et al, 2008;Ardila-Garcia et al, 2010;Cardoso et al, 2012;Gregory, 2015). Three different techniques have been used to quantify the genome of ants as: flow cytometry (FCM), Feulgen image analysis densitometry (FIAD), and, more recently, through genomics (reviewed in Nygaard & Yannick, 2015).…”

Section: Sociobiologymentioning

confidence: 99%

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Estimation of Nuclear Genome Size of Three Species of Camponotus (Mayr, 1861) (Hymenoptera: Formicidae: Formicinae) and Their Cytogenetic Relationship

et al. 2016

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The chromosome variability among ant species is remarkable, and the processes generating such variation are still under discussion since polyploidy has been observed in some distinct taxa. The chromosome number of species belonging to the Camponotus, subgenera Myrmothrix and Myrmobrachys, are highly different, whereas, the first subgenus has double the number of chromosomes of the second. In order to test the hypothesis of chromosome number doubling through polyploidy, the genome sizes of Camponotus (Myrmothrix) rufipes, Camponotus (Myrmothrix) renggeri and Camponotus (Myrmobrachys) crassus were estimated by flow cytometry. The chromosome number of specimens from the nests studied was also defined. No significant variation was noted in the genome size among them. The mean haploid genome size value (1C) of workers for the three species was 286.16 Mpb (0.29 pg). The polyploidy hypothesis can be ruled out as an evolutionary step linking the karyotype variations among the three studied species since the genome size of C. crassus with 2n = 20 chromosomes was the same as that of C. rufipes and C. renggeri with 2n = 40. The lack of variation in the amount of DNA between the related species C. rufipes and C. renggeri also demonstrate that flow cytometry is not an adequate approach to distinguish them. Our results highlight the importance of combining distinct methods, DNA quantification, and cytogenetics from the same colony. Understanding the path of chromosome evolution of three species with distinct degrees of relatedness should provide further information in enriching our knowledge about the Minimum Interaction Theory.

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Estimation of nuclear genome size of the genus Mycetophylax Emery, 1913: Evidence of no whole-genome duplication in Neoattini

Cited by 9 publications

References 29 publications

The Role of Fusion in Ant Chromosome Evolution: Insights from Cytogenetic Analysis Using a Molecular Phylogenetic Approach in the Genus Mycetophylax

The Role of Fusion in Ant Chromosome Evolution: Insights from Cytogenetic Analysis Using a Molecular Phylogenetic Approach in the Genus Mycetophylax

Comparative cytogenetics and derived phylogenic relationship among Sitophilus grain weevils (Coleoptera, Curculionidae, Dryophthorinae)

Estimation of Nuclear Genome Size of Three Species of Camponotus (Mayr, 1861) (Hymenoptera: Formicidae: Formicinae) and Their Cytogenetic Relationship

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