Estimation of Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) of Antimicrobial peptides of Saccharomyces boulardii against Selected Pathogenic Strains

T.C, Venkateswarulu; Krupanidhi, S.; Mikkili, Indira; Bobby, Nazneen; Dulla, John Babu; Alugunulla, Venkata Narayana; Sweety, Dasari; Peele, K. Abraham

doi:10.33640/2405-609x.1219

Cited by 5 publications

(3 citation statements)

References 12 publications

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“…The rhamnolipid biosurfactant's minimal inhibition concentration (MIC) value was determined according to TC et al 17 . MIC was determined by a serial dilution technique using the agar diffusion method.…”

Section: Minimal Inhibition Concentration (Mic)mentioning

confidence: 99%

Antimicrobial and Antioxidant Activity of Rhamnolipids Biosurfactant is Produced by Pseudomonas aeruginosa

Abdullah Alyousif,

Al-tamimi,

Y. Al-luaibi

2023

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Rhamnolipids are the glycolipid biosurfactant produced by different Pseudomonas species; they show antimicrobial activity and antioxidant. The findings of antimicrobial activity showed the rhamnolipid biosurfactant had an antimicrobial effect against the microorganisms at different concentrations, such as toward Bacillus cereus and Klebsiella pneumoniae, while a lower inhibitory effect toward Staphylococcus aureus and Pseudomonas aeruginosa. The rhamnolipid biosurfactant was shown to have a lower inhibitory effect against fungal strains Candida albicans and Aspergillus niger. The lower minimum inhibitory concentration (MIC) values of rhamnolipid biosurfactant toward the investigated microorganisms were 2 mg/ml for E. coli, Klebsiella pneumoniae and Pseudomonas aeruginosa and 3 mg/ml for Staphylococcus aureus, Enterobacter cloacae, Bacillus cereus, Proteus mirabilis, Candida albicans and Aspergillus niger. Rhamnolipid biosurfactant was tested as an antioxidant agent; the results showed 22.7 %, 47.4 %, 79.8 %, 85 % and 91.4 % of antioxidant activity at concentrations 5, 10. 15, 20 and 25 mg/ml, respectively. Cytotoxicity of the rhamnolipid biosurfactant was also examined at different concentrations against human erythrocytes. Hemolysis of the erythrocytes was observed at concentrations 100, 75, 50, 40 and 35 mg/ml, whereas the results exhibited no hemolysis at concentrations 25 and 15 mg/ ml. The study concluded that rhamnolipid biosurfactant showed effective antioxidant activity, no hemolysis at lower concentrations and has a high antimicrobial effect. The rhamnolipid biosurfactant is a suitable and great alternative to be employed as an effective and safe therapeutic agent. Keywords: Antimicrobial; Antioxidant; Cytotoxicity; Rhamnolipid; Pseudomonas aeruginosa

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Section: Minimal Inhibition Concentration (Mic)mentioning

confidence: 99%

Antimicrobial and Antioxidant Activity of Rhamnolipids Biosurfactant is Produced by Pseudomonas aeruginosa

Abdullah Alyousif,

Al-tamimi,

Y. Al-luaibi

2023

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“…самими складними являються продукти життєдіяльності L. rhamnosus [7,9]. Встановлений різний склад компонентів в речовинах пробіотичних сахароміцетів співпадає з роботами авторів [11][12][13]. Так, за допомогою ультрафільтрації через діалізні мембрани з порами 10 кДа отримано чотири ізоляти з протимікробною активністю (50, 75 та 100 мкл концентрованого препарату інгібувало Bacillus cereus на 26, 29 та 33 мм відповідно).…”

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“…В іншій роботі бактеріоцини S. boulardii очищали використовуючи колонку Sephadex G-50. Протимікробною активністю з зоною інгібування росту Streptococcus pneumoniae 26 мм володів компонент з молекулярною масою 18 кДа [12]. Фракціонування на колонці Sephadex G-100 показало наявність різних фракцій з відмінною активністю відносно Staphylococcus aureus, E.coli, C. albicans, Aspergillus niger [11], що співпадає з власними результатами.…”

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Gel Chromatographic Fractionation of Metabolic Complexes of L. rhamnosus GG and S. boulardii and their Antimicrobial Activity

Isaуenko¹,

Babych²,

Gorbach³

et al. 2020

Ukr. ž. med. bìol. sportu

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Pyrolysis of lemon peel waste in a fixed-bed reactor and characterization of innovative pyrolytic products

Abidi

Trabelsi

Boudhrioua³

2022

J Mater Cycles Waste Manag

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The pyrolysis of LPW was carried out in a laboratory fixed-bed reactor at final temperature of 300°C, 400°C and 500°C with an incremental heating rate of 10°C/min, under N2 atmosphere. The maximum yields of bio-oil, biochar and gas were 16.66 wt.% (at 400°C), 66.89 wt.% (300°C) and 54.6 wt.% (500°C), respectively. The recovered biochar FTIR characterization reveals that it is a promising precursor to produce carbon materials, biofertilizer and for solid fuel applications. The bio-oil chemical characterization (GC-MS and FTIR analyzes) shows its richness with innovative compounds such as squalene, d-limonene, ß-Sitosterol and phenol, suitable for applications agriculture, biochemical and pharmaceutical industries. The pyrolytic oil presents also good properties, suitable for its use as an engine fuel or as a potential source for synthetic fuels. The recovered pyrolytic gas has a maximum calorific value around 12 MJ/kg with an average composition of CO (up to 75.87 vol.%), of CH4 (up to 5.25 vol.%) and of CnHm (up to 1.48 vol.%). The results could be applied by citrus 2 farmers and agri-food industrials for large scale application to ensure a sustainable waste management of their citrus by-products and to guarantee economic benefits.

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Estimation of Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) of Antimicrobial peptides of Saccharomyces boulardii against Selected Pathogenic Strains

Abstract: (MIC) and Minimum Bactericidal Concentration (MBC) of Antimicrobial peptides of Saccharomyces boulardii against Concentration (MBC) of Antimicrobial peptides of Saccharomyces boulardii against

Cited by 5 publications

References 12 publications

Antimicrobial and Antioxidant Activity of Rhamnolipids Biosurfactant is Produced by Pseudomonas aeruginosa

Antimicrobial and Antioxidant Activity of Rhamnolipids Biosurfactant is Produced by Pseudomonas aeruginosa

Gel Chromatographic Fractionation of Metabolic Complexes of L. rhamnosus GG and S. boulardii and their Antimicrobial Activity

Pyrolysis of lemon peel waste in a fixed-bed reactor and characterization of innovative pyrolytic products

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