Estimation of Kinetic Parameters for Rice Starch Hydrolysis Inhibited by Added Materials

Apar, Dilek Kılıç; Özbek, Belma

doi:10.1080/15397730600830039

Cited by 14 publications

(18 citation statements)

References 14 publications

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“…The relatively high K m value (50.25 mg/mL) obtained during an enzyme kinetics study (Table ) also suggested that PPA has a lower affinity to SCMD. Amylases have been shown to experience substrate inhibition when starch is used as the substrate. , Interestingly, SCMD displayed a low inhibitory constant ( K i = 16.87 mg/mL), approximately one-eighth of those of other substrates. This implies that SCMD has a stronger capacity for substrate inhibition, which might be attributed to its dense internal framework along with shortened external chains.…”

Section: Resultsmentioning

confidence: 99%

An Innovative Short-Clustered Maltodextrin as Starch Substitute for Ameliorating Postprandial Glucose Homeostasis

Kong

et al. 2020

J. Agric. Food Chem.

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Dietary starch is usually associated with elevated postprandial glycemic response. This is a potential risk factor of type 2 diabetes. Here, a 1,4-α-glucan branching enzyme (GBE) was employed to reassemble α-1,4 and α-1,6 glycosidic bonds in starch molecules. Structural characterization showed that GBE-catalyzed molecular reassembly created an innovative short-clustered maltodextrin (SCMD), which showed a dense internal framework along with shortened external chains. Such short-clustered molecules obstructed digestive enzymes attack and displayed dramatically reduced digestibility. Therefore, SCMD was served as a dietary starch substitute to improve postprandial glucose homeostasis. A 22.3% decrease in glycemic peak was therefore detected in ICR mice following SCMD intake (10.7 mmol/L), compared with that in the control (13.8 mmol/L). Moreover, an attenuated insulin response (40.5% lower than that in control) to SCMD intake was regarded suitable for diabetes management. These novel discoveries demonstrate that enzymatically rebuilding starch molecules may be a meaningful strategy for diabetes management.

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Section: Resultsmentioning

confidence: 99%

An Innovative Short-Clustered Maltodextrin as Starch Substitute for Ameliorating Postprandial Glucose Homeostasis

Kong

et al. 2020

J. Agric. Food Chem.

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“…Under this condition, a higher amount of reducing sugars generated was obtained (4.9 g), in comparison with the other flow ratios analyzed (2.92 g at 250/400 and 2.86 g at 400/400). This fact can be explained by the higher rate of starch hydrolysis attributed to the prompt transfer of hydrolytic products with molecular weight lower than 20 kDa, since it is known that compounds produced during the starch hydrolysis (e.g., glucose and maltose) can induce uncompetitive inhibition of the catalytic action of α-amylase [ 1 , 51 ]. Alternatively, the rapid starch hydrolysis in the feed tank and subsequent removal of the reducing sugars generated could be related to the higher mass transfer as the feed viscosity reduces, since at low viscosity starch and α-amylase can move freely to contact each other.…”

Section: Resultsmentioning

confidence: 99%

Simulation of Human Small Intestinal Digestion of Starch Using an In Vitro System Based on a Dialysis Membrane Process

Gonzalez

González

Zúñiga

et al. 2020

Foods

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This work deepens our understanding of starch digestion and the consequent absorption of hydrolytic products generated in the human small intestine. Gelatinized starch dispersions were digested with α-amylase in an in vitro intestinal digestion system (i-IDS) based on a dialysis membrane process. This study innovates with respect to the existing literature, because it considers the impact of simultaneous digestion and absorption processes occurring during the intestinal digestion of starchy foods and adopts phenomenological models that deal in a more realistic manner with the behavior found in the small intestine. Operating the i-IDS at different flow/dialysate flow ratios resulted in distinct generation and transfer curves of reducing sugars mass. This indicates that the operating conditions affected the mass transfer by diffusion and convection. However, the transfer process was also affected by membrane fouling, a dynamic phenomenon that occurred in the i-IDS. The experimental results were extrapolated to the human small intestine, where the times reached to transfer the hydrolytic products ranged between 30 and 64 min, according to the flow ratio used. We consider that the i-IDS is a versatile system that can be used for assessing and/or comparing digestion and absorption behaviors of different starch-based food matrices as found in the human small intestine, but the formation and interpretation of membrane fouling requires further studies for a better understanding at physiological level. In addition, further studies with the i-IDS are required if food matrices based on fat, proteins or more complex carbohydrates are of interest for testing. Moreover, a next improvement step of the i-IDS must include the simulation of some physiological events (e.g., electrolytes addition, enzyme activities, bile, dilution and pH) occurring in the human small intestine, in order to improve the comparison with in vivo data.

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“…It is well-known that there are two or more stages (rapidly digested starch, RDS, and slowly digested starch, SDS) during starch digestion by hydrolases. 19,32,33 However, kinetic models that are currently used to fit starch digestion curves disregard the deviation from normal Michaelis-Menten kinetics.…”

Section: Resultsmentioning

confidence: 99%

“…Studies that have recorded the time courses 21,[31][32][33][34] for the digestion of starch display stages that are separated by a discontinuity in the smoothness of the kinetic curves of sugar production. Furthermore, the amount of time taken for the RDS to be digested can vary depending on the conditions of digestion and will not always occur in the first 20 min.…”

Section: Introductionmentioning

confidence: 99%

Kinetics of In Vitro Digestion of Starches Monitored by Time-Resolved¹H Nuclear Magnetic Resonance

Dona¹,

Pagès²,

Gilbert³

et al. 2009

Biomacromolecules

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A (1)H NMR method is presented that monitors the initial and later stages of in vitro enzymatic digestion of starch suspensions. It allows, for the first time to our knowledge, the accurate analysis of the initial 5% of the extent of hydrolysis. This is significant because rapidly digested starch produces glucose that determines the blood glucose concentration immediately after ingestion of food. The two key hydrolytic enzymes, alpha-amylase and amyloglucosidase, showed clear systematic deviation from Michaelis-Menten kinetics as the starch or wheat flour substrate that was used changed its character during the reaction. Estimates of Michaelis-Menten parameters for amyloglucosidase and alpha-amylase were successfully found by analyzing two stages of digestion separately. The Michaelis-Menten constants for purified starch were (6.4 +/- 0.8) and (1.1 +/- 0.3) g dL(-1) (% w/v), respectively; and the maximum velocities of glucose release by amyloglucosidase, and short oligoglucosides and glucose by alpha-amylase were (1.9 +/- 0.4) x 10(-2) and (1.6 +/- 0.2) x 10(-2) mmol L(-1) s(-1) for the first stage of digestion, and (9.0 +/- 1.0) x 10(-3) and (4.7 +/- 1.4) x 10(-3) mmol L(-1) s(-1) for the second stage, giving a ratio of the two V(max) values of 2.1 and 3.4, respectively.

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Estimation of Kinetic Parameters for Rice Starch Hydrolysis Inhibited by Added Materials

Cited by 14 publications

References 14 publications

An Innovative Short-Clustered Maltodextrin as Starch Substitute for Ameliorating Postprandial Glucose Homeostasis

An Innovative Short-Clustered Maltodextrin as Starch Substitute for Ameliorating Postprandial Glucose Homeostasis

Simulation of Human Small Intestinal Digestion of Starch Using an In Vitro System Based on a Dialysis Membrane Process

Kinetics of In Vitro Digestion of Starches Monitored by Time-Resolved¹H Nuclear Magnetic Resonance

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Estimation of Kinetic Parameters for Rice Starch Hydrolysis Inhibited by Added Materials

Cited by 14 publications

References 14 publications

An Innovative Short-Clustered Maltodextrin as Starch Substitute for Ameliorating Postprandial Glucose Homeostasis

An Innovative Short-Clustered Maltodextrin as Starch Substitute for Ameliorating Postprandial Glucose Homeostasis

Simulation of Human Small Intestinal Digestion of Starch Using an In Vitro System Based on a Dialysis Membrane Process

Kinetics of In Vitro Digestion of Starches Monitored by Time-Resolved1H Nuclear Magnetic Resonance

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Kinetics of In Vitro Digestion of Starches Monitored by Time-Resolved¹H Nuclear Magnetic Resonance