Estimation of IgG and IgM brucella antibodies in infected and non-infected persons by a radioimmune technique.

Hewitt, W G; Payne, D. J.

doi:10.1136/jcp.37.6.692

Cited by 14 publications

(10 citation statements)

References 12 publications

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“…Moreover, sensitivity of blood culture is often low, changing from 50 to 90% based on the period, Brucella species, culture medium, amount of disseminating bacteria, and the blood culture technique used [4][5][6] . Therefore, sero-diagnostic tests are mostly used especially in people with persistent infection and recurrences, and in the regions where antibodies against brucellosis are prevalent [4,[7][8][9] . Most frequently applied diagnostic tests for human brucellosis are the Serum Agglutination Test (SAT), the Coombs anti-Brucella test, the Rose Bengal test (RBT), and Complement Fixation Test (CFT) [7,8] .…”

Section: Introductionmentioning

confidence: 99%

“…Most frequently applied diagnostic tests for human brucellosis are the Serum Agglutination Test (SAT), the Coombs anti-Brucella test, the Rose Bengal test (RBT), and Complement Fixation Test (CFT) [7,8] . For a long time, radioimmunoassay [9,10] and enzyme immunoassay [7,[11][12][13][14][15] have also been used. The diagnostic performance of SAT and Brucellacapt has been studied thoroughly [16][17][18][19] and defined as useful tests.…”

Section: Introductionmentioning

confidence: 99%

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Validation of Two Rapid Serological Tests for Human Brucellosis Detection

Genç

Çetinkol

Yas

et al. 2019

International Journal of Medical and Dental Sciences

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As brucellosis have several non-specific clinical phases, the search for reliable diagnosis of the disease is necessary. Therefore, for serological determination of brucellosis, rapid immunofiltration tests designated as ERIFA and NERIFA and ELISAs were developed and evaluated for diagnostic purposes. The sera were collected from Clinical Microbiology Department of Kars State Hospital between 2007 and 2009. These sera were used for determination of sensitivity of ELISAs, ERIFA and NERIFA in comparison to rose bengal test, Brucellacapt and serum agglutination tests. In the study, highest sensitivity was detected by ELISA, ERIFA and NERIFA, respectively. Specificity was highest with ELISA as 95.2% and with both ERIFA and NERIFA as 92.9% and the lowest was with RBT as 90.5%. When two ELISAs were compared based on the IgG sensitivity, there was no difference (p<0.05), but the difference was important (p>0.05) when IgM was accepted as a criteria for comparison. The current study reports the first evaluation of ERIFA and NERIFA in human beings. It is concluded that the RIFA models can be accepted as an ancillary test for diagnosis of human brucellosis in endemic countries.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Validation of Two Rapid Serological Tests for Human Brucellosis Detection

Genç

Çetinkol

Yas

et al. 2019

International Journal of Medical and Dental Sciences

View full text Add to dashboard Cite

show abstract

“…The most commonly used tests are the Serum Agglutination Test (SAT), the Coombs anti-Brucella test, the Rose Bengal Test (RBT) and complement fixation. During the last decade, radioimmunoassay [7,8] and enzyme immunoassay [9][10] tests have also been used. These present technical difficulties since they require skilled personnel and high-cost material.…”

Section: Introductionmentioning

confidence: 99%

Serological Evaluation of Brucella abortus S99 Lipopolysaccharide Extracted by an Optimized Method

Salmani¹,

Siadat²,

Fallahian³

et al. 2009

American J. of Infectious Diseases

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Problem statement: Brucellosis is a globally found infectious disease and there is no licensed vaccine against human brucellosis. The present study carried-out to evaluate the potency of our modified extracted lipopolysaccharide (LPS) of B. abortus to elicit specific anti-Brucella antibodies in animal model (Rabbit) as a part of a candidate vaccine for brucellosis. Lipopolysaccharide is one of the main virulence factors and the most immunogenic structure of smooth strains of Brucella. Approach: Lipopolysaccharide of B. abortus S99 (S-LPS) initially extracted through an optimized method as described previously. After biochemical and pyrogenicity evaluations of the extracted S-LPS humoral immune response against the extracted LPS analyzed in animal model through serological assays such as Rose Bengal assay, Rapid agglutination (Rapid Wright) test and Standard agglutination test (SAT or Wright test) to demonstrate the specific elicited antibodies against the injected LPS. In addition, the interaction of LPS and anti-LPS antibodies was demonstrated by Agarose Gel Immunodiffusion (AGID) assay. Results: Higher doses of B. abortus S99 LPS caused less or equal body temperature increase in comparison to E. coli LPS doses. Sera of immunized animals had been reported positive by RBT because of B. abortus LPS immunogenicity which we extracted through our optimized method. The highest titer of anti-Brucella antibodies detected two weeks after the third immunization (assayed by rapid slide agglutination and standard agglutination tests). Anti-Brucella antibodies of immunized animals reacted more specifically with the LPS of B. abortus in comparison with E. coli LPS and precipitation lines between B. abortus LPS and immune sera appeared after 30 min while detected after three hours for E. coli LPS. Conclusions/Recommendations: The properties of B. abortus S99 LPS concluded from the present study results, suggest the possible use of this component as a carrier or a part of a sub-unit or conjugated vaccine for human brucellosis.

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“…The most commonly used tests are the serum agglutination test (SAT), the Coombs anti-Brucella test, the Rose Bengal test, and complement fixation. During the last decade, radioimmunoassay (24,28) and enzyme immunoassay (6,21,34) tests have also been used. These present technical difficulties since they require skilled personnel and high-cost material.…”

mentioning

confidence: 99%

Evaluation of an Immunocapture-Agglutination Test (Brucellacapt) for Serodiagnosis of Human Brucellosis

Orduña¹,

Almaraz²,

Prado³

et al. 2000

J Clin Microbiol

114

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We evaluated the validity and the usefulness of a new test for the diagnosis of human brucellosis based on an immunocapture-agglutination technique. A total of 315 sera from 82 patients with a diagnosis of brucellosis, 157 sera from patients in whom brucellosis was suspected but not confirmed, and 412 sera from people living in rural areas with endemic brucellosis were studied. The seroagglutination test (SAT), Coombs anti-Brucella test, and Brucellacapt test were evaluated. All the initial sera from the 82 patients proved to be positive in Brucellacapt and Coombs tests, while only 75 (91.4%) were positive in the SAT. If a ≥1/160 diagnostic threshold titer was defined for the Brucellacapt test, Coombs test, and SAT, the sensitivities were 95.1, 91.5, and 65.8%, respectively. Taking the same diagnostic threshold titer for the 157 sera from the unconfirmed but suspected patients, the specificities of the Brucellacapt, Coombs, and SAT were 81.5, 96.2, and 100%, respectively; for the 412 control sera, the specificities were 99.0, 99.8, and 100%. The diagnostic efficiency (area below the receiver operating characteristic curve) of Brucellacapt was 0.987852 (95% confidence interval [CI], 0.95109 to 0.99286), very similar to the diagnostic efficiency of the Coombs test (0.97611; 95% CI, 0.94781 to 0.99146) and higher than that of SAT (0.91013; 95% CI, 0.86649 to 0.94317). The results of the Brucellacapt test were compared with those of the Coombs test (correlation coefficient, 0.956; P= 0.000) and SAT (correlation coefficient, 0.866; P = 0.000). The study shows very good correlation between the Brucellacapt and Coombs tests, with a high concordance between titers obtained in the two tests. Nevertheless, lower correlation and concordance were found between the Brucellacapt and Coombs tests when the results for titers of ≥1/160 were compared (0.692; P = 0.000). In acute brucellosis, the Brucellacapt and Coombs tests render positive titers of ≥1/160. When the titers are lower, they increase significantly in the following 30 days, despite the evolution of SAT titers. In contrast, Brucellacapt and Coombs titers are always high (≥1/640) in brucellosis with long evolution, whether SAT titers are higher or lower than 1/160.

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Estimation of IgG and IgM brucella antibodies in infected and non-infected persons by a radioimmune technique.

Cited by 14 publications

References 12 publications

Validation of Two Rapid Serological Tests for Human Brucellosis Detection

Validation of Two Rapid Serological Tests for Human Brucellosis Detection

Serological Evaluation of Brucella abortus S99 Lipopolysaccharide Extracted by an Optimized Method

Evaluation of an Immunocapture-Agglutination Test (Brucellacapt) for Serodiagnosis of Human Brucellosis

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