Estimation of high-resolution HLA-A, -B, -C, -DRB1 allele and haplotype frequencies based on 8862 German stem cell donors and implications for strategic donor registry planning

Schmidt, Alexander H.; Baier, Daniel M.; Solloch, Ute V.; Stahr, Andrea; Cereb, Nezih; Waßmuth, Ralf; Ehninger, Gerhard; Rutt, Claudia

doi:10.1016/j.humimm.2009.08.006

Cited by 121 publications

(136 citation statements)

References 23 publications

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“…16,18 Statistical analysis All data were analyzed using SPSS software version 16.0 (SPSS Inc., Chicago, IL, USA). Differences in skewed TRBV frequencies between PBMC and CD4 1 T-cell and CD8 1 T-cell populations from recovered AHI subjects were analyzed using the Kruskal-Wallis H test or the Mann-Whitney non-parametric U test.…”

Section: Hla Genotype Determinationmentioning

confidence: 99%

Profiling the repertoire of T-cell receptor beta-chain variable genes in peripheral blood lymphocytes from subjects who have recovered from acute hepatitis B virus infection

Yang

Chen

et al. 2014

Cell Mol Immunol

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The profile of T-cell receptor beta-chain variable (TRBV) genes usually skews in subjects with virus infection or cancer. The gene melting spectral pattern (GMSP) can be used to determine the profile of the TRBV gene family. To explore the portrait of the TRBV family in peripheral blood lymphocytes from subjects who have recovered from acute hepatitis B virus infection (AHI), peripheral blood mononuclear cells (PBMCs) were separated and further sorted into CD41 and CD8 1T-cell subsets. The molecular features of the TRBV complementary determining region 3 (CDR3) motifs were determined using GMSP analysis. When a GMSP profile showed a single peak, the monoclonally expanded TRBV gene was cloned and sequenced. Skewed expansions of multiple TRBV genes were observed among the CD4 1 and CD8 1 T-cell subsets and the PBMCs. The frequency of monoclonally expanded TRBV genes in the CD8 1 T-cell subset was significantly higher than that of the CD4 1 T-cell subset and the PBMCs. Compared to other members of the TRBV gene family, TRBV11, BV15 and BV20 were predominantly expressed in the repertoire of peripheral blood lymphocytes in recovered AHI subjects. The relatively conserved amino acid motifs of TRBV5.1 and BV20 CDR3 were also detected in the CD4 1 and CD8 1 T-cell subsets. These results demonstrate the presence of multiple biased TRBV families in recovered AHI subjects. TRBV11, BV15 and BV20, especially from the CD8 1 T-cell subset, may be relevant to the pathogenesis of subjects with AHI. The preferentially selected TRBV5.1 and BV20 with the relatively conserved CDR3 motif may be potential targets for personalized treatments of chronic HBV infection.

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Section: Hla Genotype Determinationmentioning

confidence: 99%

Profiling the repertoire of T-cell receptor beta-chain variable genes in peripheral blood lymphocytes from subjects who have recovered from acute hepatitis B virus infection

Yang

Chen

et al. 2014

Cell Mol Immunol

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“…does not have statistics on the success rates of mixed race patients." Several studies, [4], [14], [6], [32], [1], [12], have estimated matching probabilities for persons of single races. But as far as we know, there are no published estimates of matching probabilities for persons of mixed race.…”

Section: Introductionmentioning

confidence: 99%

Stem Cell Donor Matching for Patients of Mixed Race

Bergstrom¹,

Garratt²,

Sheehan‐Connor³

2012

The B.E. Journal of Economic Analysis &Amp; Policy

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The plight of mixed-race leukemia patients unable to find stem cell donors with matching immunity types has received much media attention. Because of small samples, direct estimation of the distribution of immunity types for persons of mixed race has not been possible. However, because the alleles that control immunity type are located close together on a single chromosome, it is possible to estimate the distribution of types for mixed races, by using existing estimates of haplotype distributions for single races. We provide such estimates and calculate probabilities that persons of specific mixed-race combinations can find a match in the existing registry of potential donors. We also estimate probabilities that adding mixed-race donors to the registry will result in a life-saving match for a patient in need of a transplant and we compare the benefits and costs of adding new registrants of specified race or mixed race to the registry.

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“…Human MHC I molecules are highly polymorphic: each of the three "classical" MHC I gene loci, which encode HLA-A, -B, and -C, exists in multiple allelic variants that code for different allotypes. For each of the three loci, the most prevalent alleles typically exhibit frequencies of 15-30% in a given human population, but there are many alleles of lower frequency, and allele distributions differ across populations (21,22). Different MHC I allotypes present different antigenic peptides.…”

mentioning

confidence: 99%

CD8 T Cell–Evasive Functions of Human Cytomegalovirus Display Pervasive MHC Allele Specificity, Complementarity, and Cooperativity

Ameres

Besold

Plachter

et al. 2014

The Journal of Immunology

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Immunoevasive proteins (“evasins”) of human CMV (HCMV) modulate stability and localization of MHC class I (MHC I) molecules, and their supply of antigenic peptides. However, it is largely unknown to what extent these evasins interfere with recognition by virus-specific CD8 T cells. We analyzed the recognition of HCMV-infected cells by a panel of CD8 T cells restricted through one of nine different MHC I allotypes. We employed a set of HCMV mutants deleted for three or all four of the MHC I modulatory genes US2, US3, US6, and US11. We found that different HCMV evasins exhibited different allotype-specific patterns of interference with CD8 T cell recognition of infected cells. In contrast, recognition of different epitopes presented by the same given MHC I allotype was uniformly reduced. For some allotypes, single evasins largely abolished T cell recognition; for others, a concerted action of evasins was required to abrogate recognition. In infected cells whose Ag presentation efficiency had been enhanced by IFN-γ pretreatment, HCMV evasins cooperatively impared T cell recognition for several different MHC I allotypes. T cell recognition and MHC I surface expression under influence of evasins were only partially congruent, underscoring the necessity to probe HCMV immunomodulation using specific T cells. We conclude that the CD8 T cell evasins of HCMV display MHC I allotype specificity, complementarity, and cooperativity.

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Estimation of high-resolution HLA-A, -B, -C, -DRB1 allele and haplotype frequencies based on 8862 German stem cell donors and implications for strategic donor registry planning

Cited by 121 publications

References 23 publications

Profiling the repertoire of T-cell receptor beta-chain variable genes in peripheral blood lymphocytes from subjects who have recovered from acute hepatitis B virus infection

Profiling the repertoire of T-cell receptor beta-chain variable genes in peripheral blood lymphocytes from subjects who have recovered from acute hepatitis B virus infection

Stem Cell Donor Matching for Patients of Mixed Race

CD8 T Cell–Evasive Functions of Human Cytomegalovirus Display Pervasive MHC Allele Specificity, Complementarity, and Cooperativity

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