Estimation of hepatitis E virus (HEV) pig seroprevalence using ELISA and Western blot and comparison between human and pig HEV sequences in Belgium

Thiry, Damien; Mauroy, Axel; Saegerman, Claude; Thomas, Isabelle; Wautier, Magali; Miry, C; Czaplicki, Guy; Berkvens, Dirk; Praet, Nicolas; Poel, Wim van der; Cariolet, Roland; Brochier, Bernard; Thiry, Étienne

doi:10.1016/j.vetmic.2014.06.004

Cited by 41 publications

(43 citation statements)

References 32 publications

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“…The present study demonstrates the presence of HEV in the Estonian populations of domestic pigs and wild boars. The overall seroprevalence in domestic pigs was 61.6 %, which is comparable to the situation observed in other European countries, e.g., 42.7 % in Germany and 73 % in Belgium (Dremsek et al 2013;Thiry et al 2014). The HEV seroprevalence (17.2 %) among wild boars was significantly lower than that among domestic pigs (p \ 0.0001), but in agreement with the seroprevalences of 14 % reported from France and 12 % from The Netherlands (Carpentier et al 2012;Rutjes et al 2010).…”

Section: Discussionsupporting

confidence: 77%

“…To date, there have been numerous reports suggesting the zoonotic nature of HEV in Europe (Wichmann et al 2008;Schielke et al 2009;Norder et al 2009;Thiry et al 2014;Vasickova et al 2011). The present study demonstrates the presence of HEV in the Estonian populations of domestic pigs and wild boars.…”

Section: Discussionsupporting

confidence: 66%

“…Although the bootstrap value was 66 %, somewhat below the desirable 70 %, we can assume that these strains form their own lineage. Subtype 3f is common in European pigs and has been found in France, Sweden, and Belgium (Colson et al 2010;Widen et al 2010;Thiry et al 2014). The sequence of the Japanese strain, which appeared to be similar to the farm 14 sequences, was also shown by Widen et al to cluster with Swedish as well as other European sequences (Widen et al 2010).…”

Section: Discussionmentioning

confidence: 61%

“…To date, cases of human HEV infections have been reported from a number of European countries, as well as from North America and Japan (Wichmann et al 2008;Vasickova et al 2009;Drobeniuc et al 2013;Yazaki et al 2003). Numerous reports have suggested a zoonotic nature of HEV in Europe (Wichmann et al 2008;Schielke et al 2009;Norder et al 2009;Thiry et al 2014;Vasickova et al 2011). Consumption of undercooked or raw meat of domestic pigs, wild boars, or deer appears to be a major risk factor for local HEV infection in the studied areas (Yazaki et al 2003;Wichmann et al 2008;Reuter et al 2009).…”

Section: Introductionmentioning

confidence: 99%

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Hepatitis E Virus in Domestic Pigs, Wild Boars, Pig Farm Workers, and Hunters in Estonia

et al. 2015

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While hepatitis E is a growing health concern in Europe, epidemiological data on hepatitis E virus (HEV) in Estonia are scarce. Along with imported HEV infections, autochthonous cases are reported from European countries. Both domestic and wild animals can be a source of human cases of this zoonosis. Here, we investigated the presence of anti-HEV antibodies and HEV RNA in domestic pigs and wild boars, as well as in pig farm workers and hunters in Estonia. Anti-HEV antibodies were detected in 234/380 (61.6 %) of sera from domestic pigs and in all investigated herds, and in 81/471 (17.2 %) of meat juice samples from wild boars. HEV RNA was detected by real-time PCR in 103/449 (22.9 %) of fecal samples from younger domestic pigs and 13/81 (16.0 %) of anti-HEV-positive wild boar samples. Analysis of sera from 67 pig farm workers and 144 hunters revealed the presence of HEV-specific IgG in 13.4 and 4.2 % of the samples, respectively. No HEV RNA was detected in the human serum samples. Phylogenetic analyses of HEV sequences from domestic pigs and wild boars, based on a 245 bp fragment from the open reading frame 2 showed that all of them belonged to genotype 3. The present study demonstrates the presence of HEV in Estonian domestic pig and wild boar populations, as well as in humans who have direct regular contact with these animals. Our results suggest that HEV infections are present in Estonia and require attention.

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Section: Discussionsupporting

confidence: 77%

Section: Discussionsupporting

confidence: 66%

Section: Discussionmentioning

confidence: 61%

Section: Introductionmentioning

confidence: 99%

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Hepatitis E Virus in Domestic Pigs, Wild Boars, Pig Farm Workers, and Hunters in Estonia

et al. 2015

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“…Spanish pork production comes behind only China, the USA and Germany. It is well known that swine is one of the main hosts for HEV in developed countries, with a seroprevalence of 45–75% reported for European pig farms (Casas et al., ; Kantala, Heinonen, Oristo, von Bonsdorff, & Maunula, ; Thiry et al., ). Evidence of HEV has been found in fresh meat or sausages derived from these farms (Di Bartolo, Angeloni, Ponterio, Ostanello, & Ruggeri, ; Di Bartolo et al., ).…”

Section: Discussionmentioning

confidence: 99%

Rural habitat as risk factor for hepatitis E virus seroconversion in HIV‐infected patients: A prospective longitudinal study

Rivero‐Juárez

Cuenca-López

Martínez-Peinado

et al. 2017

Zoonoses and Public Health

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Our objective was to determine the incidence and clinical manifestations of acute hepatitis E virus (HEV) in HIV-infected patients. A prospective longitudinal study including HIV-infected HEV-seronegative patients was conducted; HEV seroconversion (to IgG and/or IgM) was the main outcome variable. All patients were tested for HEV antibodies every 3-6 months. For patients who developed HEV seroconversion, a data collection protocol was followed to identify associated clinical manifestations and analytical alterations. A total of 627 patients (89.9%) were followed during a median of 11.96 months (IQR: 8.52-14.52 months) and formed the study population. Forty-one patients developed detectable anti-HEV antibodies (7.2 cases per 100 patients/year). Our study found a high incidence of HEV in HIV-infected patients in southern Spain strongly associated with a rural habitat.

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Seroprevalence of hepatitis E virus infection among dogs in several developed cities in the Guangdong province of China

Wang

Zheng

et al. 2016

J. Med. Virol.

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Hepatitis E virus (HEV), as a zoonotic disease virus, was seldom studied in dogs especially in stray dogs. As previously reported, dog might be an accidental host of HEV for human beings and some risk factors might play an important role in HEV transmission. Thus, we designed this study to evaluate the seroprevalence of HEV infection among dogs in several cities in Guangdong province of China. This surveillance may help us understand risk factors including location, gender, live type, and diet habit for HEV transmission. The overall seroprevalence of anti-HEV antibodies in dogs was 19.00%. Positive rate of anti-HEV antibodies in other food groups (21.13%) was higher than that in dog food groups (9.77%) (P < 0.05), which suggested that diet habit might be a vital element of infecting HEV for dogs and play an important role in living environment. However, the analysis indicated that no strong relationship was observed among different cities, gender groups, and live type. Our study demonstrated that HEV is prevalent in dogs in the Guangdong province of China. As diet habit might become a vital element of infecting HEV for dogs and play an important role in living environment, similar studies of dogs should be conducted in the future. J. Med. Virol. 88:1404-1407, 2016. © 2016 Wiley Periodicals, Inc.

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Estimation of hepatitis E virus (HEV) pig seroprevalence using ELISA and Western blot and comparison between human and pig HEV sequences in Belgium

Cited by 41 publications

References 32 publications

Hepatitis E Virus in Domestic Pigs, Wild Boars, Pig Farm Workers, and Hunters in Estonia

Hepatitis E Virus in Domestic Pigs, Wild Boars, Pig Farm Workers, and Hunters in Estonia

Rural habitat as risk factor for hepatitis E virus seroconversion in HIV‐infected patients: A prospective longitudinal study

Seroprevalence of hepatitis E virus infection among dogs in several developed cities in the Guangdong province of China

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