Estimation of heat and mass transfer coefficients in a pilot packed-bed solid-state fermentation bioreactor

Finkler, Anelize Terezinha Jung; Weber, Mariana Zanlorenzi; Fuchs, Gustavo Alexandre; Scholz, Leandro Aluisio; Luz, Luiz Fernando de Lima; Krieger, Nádia; Mitchell, David A.; Jorge, Luíz Mário de Matos

doi:10.1016/j.cej.2020.127246

Cited by 14 publications

(9 citation statements)

References 32 publications

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“…Yet, it should be noted that this profile does not fully capture the death phase. Finkler et al (2021b) found a growth slowdown after the exponential phase, consistent with our observations, suggesting heat and mass transfer's role in non-growing conditions. Sentis-More et al, (2023) highlighted the essential role of temperature control in tray fermenters to optimize Rhizopus oryzae growth on oilseed meals during solid-state fermentation, recognizing significant microbial growth variations caused by temperature fluctuations, thereby impacting protein quality.…”

Section: Describing Fungal Growth Through Heat Evolution Patternssupporting

confidence: 91%

Maximizing Fungal Growth in State-of-the-Art Tray Solid-State Fermenter: Aeration Strategies Optimized for Enhanced Performance

Manan,

Webb

2024

American Journal of Biochemistry and Biotechnology

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This study introduced a tray fermenter, featuring eight layers of squared perforated trays, specifically designed for solid-state fermentation applications. It is named the multi-layer squared tray solid-state fermenter. The fermenter employs two aeration strategies with varying air flow rates to optimize fungal growth. Firstly, sterilized moistened air at 8 L/min flows from the bottom through the perforated trays. Secondly, a flow rate of 1 L/min delivers sterilized moistened air directly onto the substrate surface. Real-time monitoring of fungal growth is enabled by an oxygen/carbon dioxide gas analyzer and a thermocouple, tracking oxygen consumption, carbon dioxide production and metabolic heat generation. Aspergillus awamori and Aspergillus oryzae thrive on wheat bran substrate within this fermenter. Carbon dioxide evolution, indicative of fungal activity, is modeled using a Gompertz model, showing strong agreement and fitting well with experimental data. The respiratory quotient, calculated from carbon dioxide evolution and oxygen uptake rates, confirms fungal activity levels. Additionally, the correlation between fungal metabolic heat generation and carbon dioxide evolution offers complementary insights into growth dynamics. Aeration strategies notably affected fungal productivity. Both A. awamori and A. oryzae showed higher spore production with moistened air at 1 L/min. A. awamori exhibits strong enzyme production, particularly glucoamylase, xylanase and cellulase, while A. oryzae, excelled in protease production under similar conditions. Continuous monitoring of environmental variables ensures precise control of fermentation conditions. These initial findings highlight the potential of the multi-layer squared tray solid-state fermenter, with its forced moistened aeration, as a promising instrument for solid-state fermentation processes.

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Section: Describing Fungal Growth Through Heat Evolution Patternssupporting

confidence: 91%

Maximizing Fungal Growth in State-of-the-Art Tray Solid-State Fermenter: Aeration Strategies Optimized for Enhanced Performance

Manan,

Webb

2024

American Journal of Biochemistry and Biotechnology

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“…However, this profile cannot provide a complete representation that also includes the death phase. Instead, it clearly shows that the growth becomes slower after the exponential phase, as reported by Finkler et al [72] on their investigation of heat and mass transfer done in the absence of growth. Furthermore, research on hydrodynamics under abiotic and biotic conditions in a novel bench-scaled wall-cooled tray SSB packed with moistened particles of agro-industrial waste, provided a focused understanding of the interaction with heat and mass transport mechanisms [73].…”

Section: Heat Evolution As a Mechanism To Describe Fungal Growthsupporting

confidence: 55%

Performance of Fungal Growth Through Integrated Gompertz Model and Respiratory Quotient by Solid State Fermentation in Multi-Layer Squared Tray Solid State Bioreactor with Aeration Strategies

Manan

Webb

2021

Preprint

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A newly designed, laboratory-scaled and multi-layer squared tray solid state bioreactor (SSB), was developed and successfully operated in solid state fermentation (SSF) conditions. The bioreactor was divided into eight layers of squared perforated trays. Wheat bran was used as a solid substrate for the growth of Aspergillus awamori and Aspergillus oryzae. The SSB was equipped with an oxygen (O2)/carbon dioxide (CO2) gas analyser and a thermocouple. Continuous on-line monitoring of fungal growth could be performed by indirect methods that measure O2 consumed, production of CO2 and metabolic heat. The advantage of using this method is that there are no tedious and time-consuming sampling processes. The evolution of CO2, which represents an accumulation term, was integrated with time and fitted to the Gompertz model in a log-like equation. The Gompertz model generated values that may be used to stimulate and verify the experimental data. Results strongly suggest that the evolved and accumulated CO2, excellently described fungal growth. Simulated results agreed with experimental results. The respiratory quotient (RQ), which is the ratio of CO2 evolution rate (CER) to O2 uptake rate (OUR), was determined by the gas balance method. CER and OUR confirmed that measurements correlated to fungal activity. Each RQ values can explain the differences of each SFF process carried out. Yet, heat evolved by fungal activity also described fungal growth. The current findings is an excellent pre-liminary experimental work, evidencing that multi-layer squared tray SSB with forced moistened aeration present a promising alternative of instrumented bioreactors for SSF processes.

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“…Very few recent publications studied heat transfers within an SSF system [38] , [39] , [40] , [41] . These studies are based on modeling allowing more precise control of the equipment during process.…”

Section: Resultsmentioning

confidence: 99%

A novel method to assess heat transfer and impact of relevant physicochemical parameters for the scaling up of solid state fermentation systems

VAURIS

VALCAUDA²,

Husson

et al. 2022

Biotechnology Reports

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Estimation of heat and mass transfer coefficients in a pilot packed-bed solid-state fermentation bioreactor

Cited by 14 publications

References 32 publications

Maximizing Fungal Growth in State-of-the-Art Tray Solid-State Fermenter: Aeration Strategies Optimized for Enhanced Performance

Maximizing Fungal Growth in State-of-the-Art Tray Solid-State Fermenter: Aeration Strategies Optimized for Enhanced Performance

Performance of Fungal Growth Through Integrated Gompertz Model and Respiratory Quotient by Solid State Fermentation in Multi-Layer Squared Tray Solid State Bioreactor with Aeration Strategies

A novel method to assess heat transfer and impact of relevant physicochemical parameters for the scaling up of solid state fermentation systems

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