Estimation of glomerular filtration rate in dogs by plasma clearance of gadolinium diethylenetriamine pentaacetic acid as measured by use of an ELISA

Abstract

OBJECTIVE-To evaluate use of gadolinium diethylenetriamine pentaacetic acid (Gd-DTPA) to estimate glomerular filtration rate (GFR) by plasma clearance and use of an ELISA as the method of Gd-DTPA quantification. ANIMALS-16 dogs of various sexes and breeds (12 dogs were clinically normal, and 4 dogs were polyuric and polydipsic with no other clinical or biochemical abnormalities). PROCEDURES-GFR was estimated by measuring the plasma clearance of Gd-DTPA and iohexol by use of an ELISA and high-performance liquid chromatography (HPLC), respectively. The GFR was determined by use of a 1-compartment model for both methods. The GFRs obtained by Gd-DTPA plasma clearance were compared with those obtained by iohexol plasma clearance by use of correlation analysis, paired t tests, and limits of agreement analysis. A paired t test was used to evaluate differences between the 2 plasma clearance methods. RESULTS-A strong linear correlation (r(2) = 0.90) was found between GFRs derived from the plasma clearance of Gd-DTPA and those derived from the plasma clearance of iohexol. By use of limits of agreement analysis, almost all (13/14) dogs had Gd-DTPA GFRs that were within 12% of iohexol GFRs. The remaining dog had a Gd-DTPA GFR that was 45% higher than the iohexol GFR. There was no significant difference between Gd-DTPA GFRs and those obtained with iohexol. CONCLUSIONS AND CLINICAL RELEVANCE-This study revealed that plasma clearance of Gd-DTPA measured by use of an ELISA is an effective method to estimate GFR in dogs because it compared favorably with results for the iohexol-HPLC method.

γ‐secretase inhibitor DAPT mitigates cisplatin‐induced acute kidney injury by suppressing Notch1 signaling

“…GFR was evaluated using the FIT‐GFR Inulin Kit and a one‐compartment plasma clearance method (BioPhysics Assay Laboratory; BioPAL, Worcester, MA, USA) according to the manufacturer's instructions and as previously described . Briefly, mice were injected with GFR‐grade inulin (5 mg/kg; IP).…”

An overview of glomerular filtration rate testing in dogs and cats

“…In cats, two-and four-sample plasma clearance of iohexol has also provided accurate results when compared with plasma clearance of exogenous creatinine (Heiene et al, 2009). Finally, gadolinium, a contrast agent used for magnetic resonance imaging contrast studies, may be useful as an alternative marker to iohexol either via direct measurement of plasma clearance or when bound to a radionuclide, but studies are limited at this time (Magnotti et al, 2009; Nolan et al, 2009). …”

Atgl deficiency induces podocyte apoptosis and leads to glomerular filtration barrier damage

“…We applied magnetic resonance imaging (MRI) to further estimate the integrity of the glomerular filtration barrier in Atgl(À/À) mice. Gadopentetic acid (GdDTPA) is excreted by glomerular filtration, and it has been reported that the plasma clearance of GdDTPA is an effective method to estimate glomerular filtration rate (GFR) [17]. Figure 3A,B shows inversion-recovery T1 maps in which T1 relaxation time was inversely proportional to the content of GdDTPA retained in the kidney tissue.…”