Estimation of genotoxic and mutagenic potential of latex and methanolic leaves extract of Euphorbia helioscopia by comet assay and Ames test

Saleem, Uzma; Mahmood, Saeed; Ahmad, Bashir; Saleem, Mohammad; Anjum, Aftab Ahmad

doi:10.1016/s2222-1808(15)60877-5

Cited by 4 publications

(2 citation statements)

References 46 publications

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“…Genotoxicity studies on K. pinnata extract in human lymphocytes were assessed by SCGE (COMET assay) are a very sensitive test for the quantification of DNA damage and provides the direct determination of single-double strand breaks in individual cells. Cells were treated with hydrogen peroxide as positive control and different concentration of K. pinnata extract (10-100 µg/ml) results showed that there was low, medium, and high damage at concentration 10-100 µg/ml in preliminary comet assay it may be due to difference in cell physiology like cell cycle [23][24][25][26][27]. The K. pinnata extracts showed DNA damage with positive control.…”

Section: Resultsmentioning

confidence: 99%

Evaluation of Genotoxic Effects of Alcoholic Extract of Kalanchoe Pinnata Using Single Cell Gel Electrophoresis

Saravanan

Jsi³

et al. 2018

Asian J Pharm Clin Res

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Objective: The objective of the study was to evaluate the genotoxic and mutagenic potentials of Kalanchoe pinnata alcoholic leaves extract.Methods: Single cell gel electrophoresis (COMET assay) was adopted to investigate genotoxicity potential of standardized ethanolic leaves extract at the concentration of 10, 50, and 100 µg/ml, respectively, using human lymphocytes.Results: K. pinnata leaves extract did not completely damage DNA in human lymphocytes but low, medium, and high-level damage was found in with positive control. The DNA damage level decreases in low, medium, and high (12±1, 6±1, and 1±1) without positive control at the concentration of 100 µg/ml. Conclusion:It was concluded that the parent material did not induce mutagenic and genotoxic potentials at the concentrations 10, 50, and 100 µg/ml but its metabolites are inducing genotoxicity in various models.

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Section: Resultsmentioning

confidence: 99%

Evaluation of Genotoxic Effects of Alcoholic Extract of Kalanchoe Pinnata Using Single Cell Gel Electrophoresis

Saravanan

Jsi³

et al. 2018

Asian J Pharm Clin Res

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“…It gave excellent antihyperglycaemic activity (Abou-El-Hamd et al, 2012) but there are no any in vivo studies confirmed that. The ethanolic extract of Euphorbia helioscopia was approved to be safe and showed no mutagenic and genotoxic potentials at probably high concentrations (Saleem et al, 2015). This encourages us to investigate antidiabetic, antioxidant and antiapoptotic effects of this plant naturally grown in Egypt.…”

Section: A B C Dmentioning

confidence: 97%

Impact of Euphorbia helioscopia Extract Administration on Diabetes Induced by Alloxan in Mice

Beltagy¹,

Beltagy²,

Ramadan³

et al. 2020

OnLine Journal of Biological Sciences

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Evaluation of the probable synergistic toxicity of selected potentiated antiretroviral and antibiotics on some aquatic biomarker organisms

Omotola,

Genthe,

Ndlela

et al. 2023

Environ Monit Assess

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Environmental effects of active pharmaceutical compounds (APCs) in the environment are not well characterized, hence the need for comprehensive evaluation. This study employed three bioassays using three organisms, namely, Allium cepa, Daphnia magna, and Salmonella typhimurium, in the ecotoxicity study of lone and a mixture of selected APCs, namely, lamivudine (L), an antiretroviral, and ciprofloxacin (C) and sulfamethoxazole (S), antibiotics, at a concentration range between 10 and 100 ppb, in order to evaluate the potential of the lone and ternary mixture to exert synergistic toxicity. Study results from exposure to lone APCs showed that the L, C, and S trio individually had fatal impacts on daphnids, with mortality rates of 100, 75, and 95%, respectively, after 48 h. Sulfamethoxazole showed a mutagenic tendency, with a mutation ratio (background/sample ratio) of 2.0. Lamivudine showed a lethal impact on the root length of A. cepa (p > 0.05, p = 3.60E–3). Further microscopic examination of the A. cepa root tip revealed chromosomal aberrations on exposure to each compound. The LCS-mix ecotoxicology bioassays indicated a synergistic effect on the daphnids, probably due to potentiation. Although the LCS mix had a cytotoxic effect (evidenced by the absence of bacteria colonies) on exposed TA 98 P450 Salmonella typhimurium strain, this effect was not observed in other bacterial strains. Microscopic examination of A. cepa exposed to the LCS-mix revealed an aberration in the mitotic stage of the cell. The impact of combination of the pharmaceuticals in aqueous ecosystems was greater than when exposed to the tested individual pharmaceutical compounds. Study result showed that these compounds have tendencies to pose a higher risk to exposed living entities when in combined/potentiated forms, and this could lead to distortion of the regular functioning of the ecosystem, particularly bacterial and other microbial populations that are listed among primary producers of the aquatic food web.

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Estimation of genotoxic and mutagenic potential of latex and methanolic leaves extract of Euphorbia helioscopia by comet assay and Ames test

Cited by 4 publications

References 46 publications

Evaluation of Genotoxic Effects of Alcoholic Extract of Kalanchoe Pinnata Using Single Cell Gel Electrophoresis

Evaluation of Genotoxic Effects of Alcoholic Extract of Kalanchoe Pinnata Using Single Cell Gel Electrophoresis

Impact of Euphorbia helioscopia Extract Administration on Diabetes Induced by Alloxan in Mice

Evaluation of the probable synergistic toxicity of selected potentiated antiretroviral and antibiotics on some aquatic biomarker organisms

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