Estimation of genetic variation in the Secretor and Lewis genes in Iranian hospitalized children

Farahmand, Mohammad; Jalilvand, Somayeh; Arashkia, Arash; Izadi, Anahita; Forouzannia, Seyed Mohammad; Mollaei-Kandelous, Yaghoub; Shoja, Zabihollah

doi:10.1016/j.tracli.2020.12.001

Cited by 3 publications

(4 citation statements)

References 36 publications

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“…Two loci must be considered when inferring phenotypes of the Lewis blood group. The secretor, Le(a-b+), and non-secretor, Le(a+b-), phenotypes are most commonly determined by a nonsense mutation (rs601338 Trp154Ter) in FUT2 on chromosome 19 32 whereas the null phenotype, Le(a-b-), is caused by a variety of different alleles in FUT3 that encode nonfunctional transferases, regardless of FUT2 genotype 33 . We inferred the secretor phenotype using Trp154Ter in FUT2 and the null phenotype using three different SNVs in FUT3 that have been associated with Le(a-b-) in an Iranian population (rs28362459 Leu20Arg, rs812936 Arg68Trp, and rs778986 Met105Thr) 33 .…”

Section: Resultsmentioning

confidence: 99%

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Characterization of Blood Group Variants in an Omani Population by Comparison of Whole Genome Sequencing and Serology

Haffener,

Al-Riyami,

Al-Zadjali

et al. 2024

Preprint

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Although blood group variation was first described over a century ago, our understanding of the genetic variation affecting antigenic expression on the red blood cell surface in many populations is lacking. This deficit limits the ability to accurately type patients, especially as serological testing is not available for all described blood groups, and targeted genotyping panels may lack rare or population-specific variants. Here, we perform serological assays across 24 antigens and whole genome sequencing on 100 Omanis, a population underrepresented in genomic databases. We inferred blood group phenotypes using the most commonly typed genetic variants. The comparison of serological to inferred phenotypes resulted in an average concordance of 96.9%. Among the 22 discordances, we identify seven known variants in four blood groups that, to our knowledge, have not been previously reported in Omanis. Incorporating these variants for phenotype inference, concordance increases to 98.8%. Additionally, we describe five candidate variants in the Lewis, Lutheran, MNS, and P1 blood groups that may affect antigenic expression, although further functional confirmation is required. Notably, we identify several blood group alleles most common in African populations, likely introduced to Oman by gene flow over the last thousand years. These findings highlight the need to evaluate individual populations and their population history when considering variants to include in genotype panels for blood group typing. This research will inform future work in blood banks and transfusion services.Key PointsUtilizing whole genome sequencing to infer blood types in Omanis demonstrates high sensitivity for most blood groupsPopulation history influences blood group variation, necessitating population-specific genotype panels

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Section: Resultsmentioning

confidence: 99%

“…with Le(a-b-) in an Iranian population (rs28362459 Leu20Arg, rs812936 Arg68Trp, and rs778986 Met105Thr) 33 . This resulted in a concordance of 96%.…”

Section: Lewis Blood Groupmentioning

confidence: 98%

Characterization of Blood Group Variants in an Omani Population by Comparison of Whole Genome Sequencing and Serology

Haffener,

Al-Riyami,

Al-Zadjali

et al. 2024

Preprint

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show abstract

“…56,57 Similarly, we also detected circulating P [8]-lineage IV in Iran more frequently in nonsecretors while P [8]-lineage III strains tended to be more common in secretor children. Thus, considering the recently reported "37% frequency" of nonsecretory phenotype in Iran 58 It might be concluded that the P[8]-lineage IV trend will be toward an increase in the context of competition with other P[8]-lineages especially P [8]-lineage III, in future.…”

Section: Discussionmentioning

confidence: 99%

Circulating rotavirus P[8]‐lineage IV, unlike P[8]‐lineage III, significantly related to nonsecretors status in Iranian children

Farahmand

Latifi

Yaeghobi

et al. 2022

Journal of Medical Virology

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Rotavirus (RV) P[8] strains are responsible for the most of the RV infections globally and are significantly associated with the secretor and Lewis positive status. Among the distinct P[8] lineages, different ligand affinities have been detected which can be linked to differences in secretor status associated histo‐blood group antigens (HBGAs). Herein, we report the lineages of P[8] strains and their associated secretor and Lewis antigen phenotypes in Iranian children. The phylogenetic tree and sequence analyses showed that the most common detected RV P[8] strain belonged to P[8]‐lineage III (92%) and were significantly associated with secretor and Lewis positive status. In contrast, 8% of P[8] strains clustered into the P[8]‐lineage IV and were significantly associated with nonsecretor status, implying that lineage IV tends to infect nonsecretor individuals. Furthermore, protein modeling and amino acid analyses of the VP8* glycan binding site of Iranian P[8]‐lineage IV strains indicated two residual substitutions (T184V and N216V/I) compared to the P[8]‐lineage III strains that might have affected the glycan affinity among P[8]‐lineages IV strains. The corresponding residual changes might permit their continued transmission in nonsecretor children in competition with other P[8]‐lineages. Although nonsecretors show natural resistant to P[8] strains, but such residual changes might overcome this natural resistance which in turn might indirectly contribute to the decline in the vaccine efficacy in populations where HBGA polymorphism allows their circulation at high frequency.

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“…Given the secretor and Lewis-positive status of most individuals in Europe, North American countries, and other Caucasians, such as Iranians, it is not surprising that the majority of rotaviruses detected in Iran belong to the P[8] genotypes. ( Carlsson et al, 2009 ; Farahmand et al, 2021a , 2021b ; Le Pendu, 2004 ). This is further supported by the fact that Rotarix and RotaTeq vaccines, which are commonly used to combat rotavirus gastroenteritis, primarily target the P[8] genotype.…”

Section: Discussionmentioning

confidence: 99%

Circulating rotavirus strains in children with acute gastroenteritis in Iran, 1986 to 2023 and their genetic/antigenic divergence compared to approved vaccines strains (Rotarix, RotaTeq, ROTAVAC, ROTASIIL) before mass vaccination: Clues for vaccination policy makers

Jalilvand,

Latifi,

Kachooei

et al. 2024

Virus Research

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Estimation of genetic variation in the Secretor and Lewis genes in Iranian hospitalized children

Cited by 3 publications

References 36 publications

Characterization of Blood Group Variants in an Omani Population by Comparison of Whole Genome Sequencing and Serology

Characterization of Blood Group Variants in an Omani Population by Comparison of Whole Genome Sequencing and Serology

Circulating rotavirus P[8]‐lineage IV, unlike P[8]‐lineage III, significantly related to nonsecretors status in Iranian children

Circulating rotavirus strains in children with acute gastroenteritis in Iran, 1986 to 2023 and their genetic/antigenic divergence compared to approved vaccines strains (Rotarix, RotaTeq, ROTAVAC, ROTASIIL) before mass vaccination: Clues for vaccination policy makers

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