Estimation of genetic diversity in varieties of Mucuna pruriens using RAPD

Padmesh, P.; Reji, J. V.; Dhar, M; Seeni, S.

doi:10.1007/s10535-006-0051-z

Cited by 43 publications

(25 citation statements)

References 16 publications

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“…Therefore, the observed high levels of polymorphism detected in otherwise self-pollinated accessions of A. rugosa may be attributable to the broad genetic base of the species that in the process of speciation may have acquired novel gene combinations for better adaptability to changing environmental conditions. A similar observation of high diversity in a selfpollinated species has been reported in Mucuna pruriens (Capo-chichi et al, 2003;Padmesh et al, 2006). The high genetic diversity observed in A. rugosa may be due to its wide geographical and climatic distribution in both tropical and temperate regions that must have accelerated the evolvement of novel and diverse genotypes.…”

Section: Discussionsupporting

confidence: 74%

Genetic Diversity and Population Structure of Korean Mint Agastache rugosa (Fisch & Meyer) Kuntze (Lamiaceae) Using ISSR Markers

Kang¹,

Suresh²,

Lee³

et al. 2013

Korean Journal of Plant Resources

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-Agastache rugosa, a member of the mint family (Labiatae), is a perennial herb widely distributed in East Asian countries. It is used in traditional medicine for the treatment of cholera, vomiting, and miasma. This study assessed the genetic diversity and population structures on 65 accessions of Korean mint A. rugosa germplasm based on inter simple sequence repeat (ISSR) markers. The selected nine ISSR primers produced reproducible polymorphic banding patterns. In total, 126 bands were scored; 119 (94.4%) were polymorphic. The number of bands generated per primer varied from 7 to 18. A minimum of seven bands was generated by primer 874, while a maximum of 18 bands was generated by the primer 844. Six primers (815, 826, 835, 844, 868, and 874) generated 100% polymorphic bands. This was supported by other parameters such as total gene diversity (HT) values, which ranged from 0.112 to 0.330 with a mean of 0.218. The effective number of alleles (NE) ranged from 1.174 to 1.486 with a mean value of 1.351. Nei's genetic diversity (H) mean value was 0.218, and Shannon's information index (I) mean value was 0.343. The high values for total gene diversity, effective number of alleles, Nei's genetic diversity, and Shannon's information index indicated substantial variations within the population. Cluster analysis showed characteristic grouping, which is not in accordance with their geographical affiliation. The implications of the results of this study in developing a strategy for the conservation and breeding of A. rugosa and other medicinal plant germplasm are discussed.

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Section: Discussionsupporting

confidence: 74%

Genetic Diversity and Population Structure of Korean Mint Agastache rugosa (Fisch & Meyer) Kuntze (Lamiaceae) Using ISSR Markers

Kang¹,

Suresh²,

Lee³

et al. 2013

Korean Journal of Plant Resources

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“…pruriens is by far the most investigated species for the study of intra species genetic and chemodiversity. Previously, many workers have studied and estimated the genetic diversity in both cultivated and wild Mucuna pruriens and its varieties using RAPD and morpho-agronomical characters, and found that M. pruriens has high genetic variation (Padmesh et al 2006;Kalidas and Mohan 2010;Sathyanarayana et al 2012). But very few studies have attempted to study the genetic diversity among the various Mucuna species.…”

Section: Discussionmentioning

confidence: 99%

Assessment of genetic diversity in Mucuna species of India using randomly amplified polymorphic DNA and inter simple sequence repeat markers

Patil

Pawar

Rane

et al. 2016

Physiol Mol Biol Plants

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Genus Mucuna which is native to China and Eastern India comprises of perennial climbing legume with long slender branches, trifoliate leaves and bear green or brown pod covered with soft or rigid hairs that cause intense irritation. The plants of this genus are agronomically and economically important and commercially cultivated in India, China and other regions of the world. The high degrees of taxonomical confusions exist in Mucuna species that make authentic identification and classification difficult. In the present study, the genetic diversity among the 59 accessions of six species and three varieties of M. pruriens has been assessed using DNA fingerprinting based molecular markers techniques namely randomly amplified polymorphic DNA (RAPD), inter simple sequence repeats (ISSR) and combined dataset of RAPD and ISSR. Also, genetic relationship among two endemic species of Mucuna namely M. imbricata and M. macrocarpa and two varieties namely IIHR hybrid (MHR) and Dhanwantari (MD) with other species under study was investigated by using cluster analysis and principal coordinate analysis.The cluster analysis of RAPD, ISSR and combined dataset of RAPD and ISSR clearly demonstrated the existence of high interspecific variation than intra-specific variation in genus Mucuna. The utility and efficacy of RAPD and ISSR for the study of intra species and interspecies genetic diversity was evident from AMOVA and PCoA analysis. This study demonstrates the genetic diversity in Mucuna species and indicates that these markers could be successfully used to assess genetic variation among the accessions of Mucuna species.

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“…Padmesh et al (2006) proved the potential use of RAPD to estimate intra-specific variation in Mucuna pruriens that could not be detected by sequence based ITS approach. Gielis et al (1997) demonstrated that the RAPD approach could generate reliable markers that are useful for establishing species relationships in Phyllostachys and bamboos in general.…”

Section: Introductionmentioning

confidence: 97%

Phylogenetic relationships among the bamboo species as revealed by morphological characters and polymorphism analyses

Das¹,

Bhattacharya²,

Basak³

et al. 2007

Biol. plant.

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Phylogenetic relationships among 15 bamboo species were evaluated using 32 key morphological descriptors and 120 polymorphic loci of the genomic DNA generated using randomly amplified polymorphic DNA (RAPD) technique. The dendrogram generated for these two parameters based on similarity matrix computed from allelic polymorphism data using unweighted pair group method of analysis. Phylogenetic relationships as revealed from the dendrogram and principal component analysis was in concurrence with the reliable, widely referred system of bamboo classification, while, the cluster pattern generated from the similarity matrix derived from key morphological character analysis was discriminatory. The findings suggest that the molecular evidences need to be supplemented by morphological data to validate the phylogenetic relationships among taxa.

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Estimation of genetic diversity in varieties of Mucuna pruriens using RAPD

Cited by 43 publications

References 16 publications

Genetic Diversity and Population Structure of Korean Mint Agastache rugosa (Fisch & Meyer) Kuntze (Lamiaceae) Using ISSR Markers

Genetic Diversity and Population Structure of Korean Mint Agastache rugosa (Fisch & Meyer) Kuntze (Lamiaceae) Using ISSR Markers

Assessment of genetic diversity in Mucuna species of India using randomly amplified polymorphic DNA and inter simple sequence repeat markers

Phylogenetic relationships among the bamboo species as revealed by morphological characters and polymorphism analyses

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