Estimation of Functional Activity of Ionized Calcium for Complement System Reactivity Assessment

Abstract

We proposed a new indicator for evaluation of functional activity of Ca(2+) in human blood serum based on lysis of sheep erythrocytes with 10% human blood serum in the presence of 0.55 mM ethylene glycol tetraacetic acid at 37°C for 10 min. After incubation, the degree of sheep erythrocytes lysis inhibition is estimated: inhibition of complement hemolytic activity <30% is considered as high functional Са(2+) activity, inhibition by 31-70% corresponds to normal activity, and >71% indicates low activity. The comparative studies of complement activating function of heterophilic antibodies, complement system reactivity in the presence of 0.29 M NaCl, and functional activity of ionized Ca(2+) make possible estimation of the individual's immune status.

Mitochondrial genome sequencing in atherosclerosis: what's next?

“…Cardiovascular diseases are currently a basic cause of mortality in highly developed countries [1][2][3][4][5][6][7][8][9][10]. The major reason for genesis and development of cardiovascular diseases is atherosclerosis [1,[11][12][13][14][15][16][17][18]. At the present time high technology methods of molecular genetic diagnostics can significantly simplify early presymptomatic recognition of patients with atherosclerosis, to detect risk groups and to perform a family analysis of this pathology [19][20][21][22][23][24][25][26][27].…”