1966
DOI: 10.1016/0003-2697(66)90138-2
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Estimation of free methylpentoses in the presence of glycosidically bound sugar

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1970
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Cited by 29 publications
(4 citation statements)
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“…Total sialic acid was assayed by a modified resorcinol method [7], where N-glycolylneuraminic acid (NGN) gives 134% of the intensity of color given by N-acetylneuraminic acid (NAN). Sugars released by acid or enzymatic hydrolysis were determined by the thiobarbituric acid proce- dure [8] for sialic acid and the Conway unit procedure for free fucose [9]. N-Glycolyl neuramic acid gives 77% of the intensity of color given by NAN in the thiobarbituric acid assay.…”
Section: Methodsmentioning
confidence: 99%
“…Total sialic acid was assayed by a modified resorcinol method [7], where N-glycolylneuraminic acid (NGN) gives 134% of the intensity of color given by N-acetylneuraminic acid (NAN). Sugars released by acid or enzymatic hydrolysis were determined by the thiobarbituric acid proce- dure [8] for sialic acid and the Conway unit procedure for free fucose [9]. N-Glycolyl neuramic acid gives 77% of the intensity of color given by NAN in the thiobarbituric acid assay.…”
Section: Methodsmentioning
confidence: 99%
“…145 ///. Free 6-deoxyhexoses can be determined readily by measuring the amount of acetaldehyde released on oxidation with periodate.…”
Section: Quantitative Analysis Of the Sugarsmentioning
confidence: 99%
“…For chromatographic procedures, the protein content was estimated by measuring the absorbance at 280 nm. The amount of free fucose was determined by the microdiffusion method 16 ) using a Conway chamber.…”
mentioning
confidence: 99%