Estimation of Focus Reduction Neutralization Test for Measurement of Neutralizing Antibody Titer against Japanese Encephalitis Virus

Watanabe, Kanako; Hirokawa, Chika; Kon, Miyako; Tamura, Tsutomu; Nishikawa, Makoto

doi:10.7883/yoken.jjid.2008.424

Cited by 7 publications

(1 citation statement)

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“…Different types of NTs based on either viral plaque or focus staining procedures have been developed for the VPDs, i.e., Measles virus (MeV), Mumps virus (MuV), Rubella (RuV) and Varicella Zoster virus [7,10,13,16,21,22,[38][39][40][41][42][43][44][45]55,56,65,68]. Similarly, many improved versions of NTs have been described for JEV, especially for vaccine and wild-type strains [14,15,[27][28][29]. The applications of various NTs have been documented for Rotaviruses that can be easily prevented by immunization [69,70].…”

Section: Measurement Of Neutralizing Antibodies To Public Health-impo...mentioning

confidence: 99%

Immuno-Colorimetric Neutralization Test: A Surrogate for Widely Used Plaque Reduction Neutralization Tests in Public Health Virology

Vaidya

2023

Viruses

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Since their first documentation in 1952, plaque reduction neutralization tests (PRNTs) have become the choice of test for the measurement of neutralizing antibodies against a particular virus. However, PRNTs can be performed only against viruses that cause cytopathic effects (CPE). PRNTs also require skilled personnel and can be time-consuming depending on the time required for the virus to cause CPE. Hence, their application limits large-scale studies or epidemiological and laboratory investigations. Since 1978, many surrogate PRNTs or immunocolorimetric assay (ICA)-based focus reduction neutralization tests (FRNT) have been developed. In this article, ICAs and their utility in FRNTs for the characterization of neutralizing antibodies, homologous or heterologous cross-neutralization, and laboratory diagnosis of viruses of public health importance have been discussed. Additionally, possible advancements and automations have been described that may help in the development and validation of novel surrogate tests for emerging viruses.

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Section: Measurement Of Neutralizing Antibodies To Public Health-impo...mentioning

confidence: 99%

Immuno-Colorimetric Neutralization Test: A Surrogate for Widely Used Plaque Reduction Neutralization Tests in Public Health Virology

Vaidya

2023

Viruses

View full text Add to dashboard Cite

show abstract

Comparison of sputum specimens and nasopharyngeal swab specimens for diagnosis of acute human metapneumovirus-related lower respiratory tract infections in adults

Yajima

Takahashi²,

Kimura³

et al. 2022

Journal of Clinical Virology

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Immunogenicity of single-dose Vero cell-derived Japanese encephalitis vaccine in Japanese adults

Takeshita

Lim

Mizuno

et al. 2014

Journal of Infection and Chemotherapy

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Estimation of Focus Reduction Neutralization Test for Measurement of Neutralizing Antibody Titer against Japanese Encephalitis Virus

Cited by 7 publications

References 0 publications

Immuno-Colorimetric Neutralization Test: A Surrogate for Widely Used Plaque Reduction Neutralization Tests in Public Health Virology

Immuno-Colorimetric Neutralization Test: A Surrogate for Widely Used Plaque Reduction Neutralization Tests in Public Health Virology

Comparison of sputum specimens and nasopharyngeal swab specimens for diagnosis of acute human metapneumovirus-related lower respiratory tract infections in adults

Immunogenicity of single-dose Vero cell-derived Japanese encephalitis vaccine in Japanese adults

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