Estimation of early postmortem intervals by a multiple regression analysis using rectal temperature and non-temperature based postmortem changes

K, Honjyo; Yonemitsu, Kosei; Tsunenari, Shigeyuki

doi:10.1016/j.jcfm.2005.02.003

Cited by 17 publications

(11 citation statements)

References 8 publications

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“…Many methods have been attempted to accurately determine the PMI. These include examination of external physical characteristics of the body, chemical changes of body fluids, stomach contents and cadaveric temperatures [1][2][3][4][5][6][7].…”

Section: Introductionmentioning

confidence: 99%

Analysis of postmortem metabolic changes in rat kidney cortex using Fourier transform infrared spectroscopy

Huang

et al. 2008

Spectroscopy

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Abstract. Estimation of the time since death (postmortem interval, PMI) is one of the most difficult problems in forensic investigations and many methods currently are utilized to estimate the PMI. The aim of this study was to investigate the changes of Fourier transform infrared (FT-IR) spectra of rat kidney cortex from time zero to 168 h postmortem at molecular level. The spectra of rat kidney cortex displayed the prominent changes with increasing postmortem interval. (1) Significant increase in the intensity of the C-H stretching bands at 2958, 2925, 2871, 2852 cm −1 , the =C-H stretching band at 3012 cm −1 , the CO 2 − symmetric stretching band at 1396 cm −1 and the N-H bend, C-N stretching at 1541 cm −1 ; (2) significant decrease in the intensity of the PO 2 − stretching band at 1238, 1080 cm −1 ; (3) the intensity of at 3303, 1652 and 1170 cm −1 remained relatively stable. The linear regression analysis of the various absorption intensity and area ratios against the PMI shows a close correlation, maximum for A 1541 /A 1396 ratio (R 2 = 0.95) and minimum for I 1080 /I 1396 ratio (R 2 = 0.70). Our results indicate that FT-IR spectroscopy may be a useful technique for estimating the short-and long-term PMI.

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Section: Introductionmentioning

confidence: 99%

Analysis of postmortem metabolic changes in rat kidney cortex using Fourier transform infrared spectroscopy

Huang

et al. 2008

Spectroscopy

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“…Os métodos de estimativa do IPM baseados na temperatura são os mais utilizados, principalmente nas primeiras 24 horas 29,30,31 . Rentoul e Smith (apud França, 2004) 21 propuseram uma fórmula simples, levando em consideração um esfriamento médio de 1,5°C por hora: H=N-C/1,5, em que H seria o tempo calculado, N a temperatura retal normal de 37,2°C e C a temperatura retal no momento do exame 21 .…”

Section: Resfriamento Cadavéricounclassified

“…Além disso, a temperatura ambiente e a quantidade de roupas que a vítima estava usando também são fatores de forte influência na modificação da temperatura corporal após a morte 38 . 30 propõe utilização de fórmula matemática para realizar a estimativa do IPM com base na temperatura retal aliada a medidas de rigidez, hipóstase e opacificação da córnea. Entretanto, relata que o trabalho apresenta grande limitação, visto que a fórmula somente pode ser aplicada em ambientes em que não haja flutuações da temperatura ambiente.…”

Section: T Zerbini and P H Nsaldivaunclassified

A Evolução Histórica das Ferramentas Disponíveis para a Cronotanagnose Inicial

Zerbini¹,

Saldiva²

2014

BJFS

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Resumo. A estimativa do intervalo postmortem é fundamental em casos de investigação criminal, pois tal estimativa pode inocentar ou culpar algum suspeito. Entretanto, apesar das técnicas utilizadas atualmente, ainda não se pode estabelecer com exatidão a hora do fato, mas sim uma aproximação em faixa de tempo segura. Diante desse cenário, o presente trabalho tem por objetivo descrever a evolução histórica dos métodos utilizados na estimativa do intervalo postmortem inicial.

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“…Many methods have been attempted to accurately determine the PMI. These include examination of external physical characteristics of the body, chemical changes in body fluids, stomach contents and cadaveric temperatures (1)(2)(3)(4)(5)(6), and analysis of potassium concentration in vitreous humor (7)(8)(9) and postmortem protein degradation within different tissues (10,11). In addition, some researchers have been focused on the degradation of nucleic acid including DNA and RNA (12,13).…”

Section: Linear Regressionmentioning

confidence: 99%

The Changes of Fourier Transform Infrared Spectrum in Rat Brain*

Wang

2012

Journal of Forensic Sciences

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Estimation of the time since death (postmortem interval [PMI]) is one of the most difficult problems in forensic investigations, and many methods currently are utilized to estimate the PMI. The goal of this study was to investigate the changes of attenuated total reflection-Fourier transform infrared (ATR-FTIR) spectra of rat brain from postmortem time 0-144 h. The intensity ratios of major absorbance bands were examined (I(1066)/I(1392), I(1168)/I(1392), I(1234)/I(1454), I(1301)/I(1392), I(1647)/I(2956), I(2921)/I(2850), and I(1647)/I(1539)). The spectra of rat brain displayed prominent changes with increasing PMI. The band at 2871 and 1737 per cm became weak with the time increasing and even disappeared at postmortem 96 and 72 h, respectively. A close linear correlation was shown between the relative absorption intensity and the PMI, and the I(1234) /I(1454) offered a stronger correlation (r = 0.973). Our results indicate that ATR-FTIR spectroscopy may be a useful technique for estimating the PMI.

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Estimation of early postmortem intervals by a multiple regression analysis using rectal temperature and non-temperature based postmortem changes

Cited by 17 publications

References 8 publications

Analysis of postmortem metabolic changes in rat kidney cortex using Fourier transform infrared spectroscopy

Analysis of postmortem metabolic changes in rat kidney cortex using Fourier transform infrared spectroscopy

A Evolução Histórica das Ferramentas Disponíveis para a Cronotanagnose Inicial

The Changes of Fourier Transform Infrared Spectrum in Rat Brain*

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