Estimation of deoxygenated and oxygenated hemoglobin by multispectral blind linear unmixing

Granados-Castro, Liliana; Gutierrez-Navarro, Omar; Cruz-Guerrero, Inés A.; Mendoza-Chavarria, Juan Nicolas; Campos-Delgado, Daniel U.

doi:10.1109/dsd57027.2022.00119

Cited by 4 publications

(1 citation statement)

References 21 publications

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“…9 (470 and 530 nm versus 450, 532, and 537 nm for hemoglobin), in Ref. 23 (660 and 880 nm versus 663, 811, and 863 nm for oxygenation), and the TIVITA Mini, the use of additional or alternative wavelengths might result in improved measurements. Tissue modeling and simulated MSI 24 – 26 as well as partial least squares regression 14 , 27 – 29 are promising wavelength selection methods and will be part of future investigations.…”

Section: Discussionmentioning

confidence: 99%

Switchable LED-based laparoscopic multispectral system for rapid high-resolution perfusion imaging

Pfahl,

Polat,

Köhler

et al. 2023

J. Biomed. Opt.

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.SignificanceMultispectral imaging (MSI) is an approach for real-time, quantitative, and non-invasive tissue perfusion measurements. Current laparoscopic systems based on mosaic sensors or filter wheels lack high spatial resolution or acceptable frame rates.AimTo develop a laparoscopic system for MSI-based color video and tissue perfusion imaging during gastrointestinal surgery without compromising spatial or temporal resolution.ApproachThe system was built with 14 switchable light-emitting diodes in the visible and near-infrared spectral range, a 4K image sensor, and a 10 mm laparoscope. Illumination patterns were created for tissue oxygenation and hemoglobin content monitoring. The system was calibrated to a clinically approved laparoscopic hyperspectral system using linear regression models and evaluated in an occlusion study with 36 volunteers.ResultsThe root mean squared errors between the MSI and reference system were 0.073 for hemoglobin content, 0.039 for oxygenation in deeper tissue layers, and 0.093 for superficial oxygenation. The spatial resolution at a working distance of 45 mm was 156 μm. The effective frame rate was 20 fps.ConclusionsHigh-resolution perfusion monitoring was successfully achieved. Hardware optimizations will increase the frame rate. Parameter optimizations through alternative illumination patterns, regression, or assumed tissue models are planned. Intraoperative measurements must confirm the suitability during surgery.

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