Estimation of dehydroascorbic acid in blood of diabetic patients

Chatterjee, I. B.; Banerjee, A.

doi:10.1016/0003-2697(79)90155-6

Cited by 71 publications

(26 citation statements)

References 12 publications

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“…In the present study the IC 50 of D-glucose on transport of 150 M DHA was 10 and 4 mM, for GLUT1 and GLUT3, respectively, which are within the physiologic range of plasma glucose. Consistent with such inhibition, higher levels of DHA have been reported in diabetic plasma (61)(62)(63)(64)(65). The significance of these findings (66,67) and whether DHA transport and cellular AA accumulation is aberrant in diabetic individuals remains to be elucidated.…”

Section: Discussionsupporting

confidence: 52%

Glucose Transporter Isoforms GLUT1 and GLUT3 Transport Dehydroascorbic Acid

Rumsey

Kwon

et al. 1997

Journal of Biological Chemistry

433

328

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Dehydroascorbic acid (DHA) is rapidly taken up by cells and reduced to ascorbic acid (AA). Using the Xenopus laevis oocyte expression system we examined transport of DHA and AA via glucose transporter isoforms GLUT1-5 and SGLT1. The apparent K m of DHA transport via GLUT1 and GLUT3 was 1.1 ؎ 0.2 and 1.7 ؎ 0.3 mM, respectively. High performance liquid chromatography analysis confirmed 100% reduction of DHA to AA within oocytes. GLUT4 transport of DHA was only 2-4-fold above control and transport kinetics could not be calculated. GLUT2, GLUT5, and SGLT1 did not transport DHA and none of the isoforms transported AA. Radiolabeled sugar transport confirmed transporter function and identity of all cDNA clones was confirmed by restriction fragment mapping. GLUT1 and GLUT3 cDNA were further verified by polymerase chain reaction. DHA transport activity in both GLUT1 and GLUT3 was inhibited by 2-deoxyglucose, D-glucose, and 3-O-methylglucose among other hexoses while fructose and L-glucose showed no inhibition. Inhibition by the endofacial inhibitor, cytochalasin B, was non-competitive and inhibition by the exofacial inhibitor, 4,6-O-ethylidene-␣-glucose, was competitive. Expressed mutant constructs of GLUT1 and GLUT3 did not transport DHA. DHA and 2-deoxyglucose uptake by Chinese hamster ovary cells overexpressing either GLUT1 or GLUT3 was increased 2-8-fold over control cells. These studies suggest GLUT1 and GLUT3 isoforms are the specific glucose transporter isoforms which mediate DHA transport and subsequent accumulation of AA. Ascorbate (AA)1 is transported across cellular membranes by two distinct mechanisms. Ascorbate itself is transported by a sodium-dependent saturable transporter which has not been isolated (1-8). Ascorbate outside cells can be oxidized to dehydroascorbic acid (DHA), which is transported by a different mechanism (7, 9 -14). Once within cells, dehydroascorbic acid is immediately reduced to ascorbate by both chemical and protein mediated processes (15-18).Dehydroascorbic acid is structurally similar to glucose.Therefore, DHA entry has been proposed to be mediated by glucose transporters (12,13,19,20). Despite investigations in several cell types, this hypothesis has not been proven. The ideal means to verify it is to express glucose transporters using an expression system, and to study DHA transport activity. If any transporters were active, transport kinetics could be characterized only under conditions of 100% internal reduction to ascorbate, consistent with DHA transport into cells being ratelimiting (7). If internal DHA reduction were incomplete, kinetics could not be calculated. Although one study characterized DHA transport by expressed GLUT1 (21), there were a number of flaws in this report. Experiments were performed using mixtures of ascorbic acid and ascorbic acid oxidase instead of pure DHA as substrate. There was insufficient data about internal DHA reduction at each external DHA concentration, and calculations of high affinity transport were based on incorrect mathematical assumptions...

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Section: Discussionsupporting

confidence: 52%

Glucose Transporter Isoforms GLUT1 and GLUT3 Transport Dehydroascorbic Acid

Rumsey

Kwon

et al. 1997

Journal of Biological Chemistry

433

328

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“…There is some disagreement in the field on what normal blood plasma levels of DHA may be. Most studies suggest that the DHA concentration in normal individuals is about 10-60% of the total blood ascorbate concentration, a figure of 10-20% probably being the most accurate (Chatterjee & Banerjee, 1979;Som et al, 1981;Sinclair et al, 1991;Deutsch & Kolhouse, 1993;Moeslinger et al, 1995;Tessier et al, 1996;Seghieri et al, 1998;Bakaev et al, 1999). There are some reports, however, that no measurable DHA is present in blood plasma.…”

Section: Hypothesis: Glucose Competes With Dha For Transport Into Celmentioning

confidence: 90%

Are Diabetic Neuropathy, Retinopathy and Nephropathy Caused by Hyperglycemic Exclusion of Dehydroascorbate Uptake by Glucose Transporters?

Root‐Bernstein

Busik

Henry

2002

Journal of Theoretical Biology

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“…Carotenoids, vitamin C, and vitamin E may have a protective effect against development of diabetes by relieving oxidative stress that interferes with the glucose uptake by cells (Halliwell & Gutteridge, 1989;Gordon, 1996). In several epidemiological studies, intakes or serum levels of vitamin C, vitamin E, or carotenoids have been inversely associated with diabetes (Chatterjee & Banerjee, 1979;Sinclair et al, 1994;Feskens et al, 1995;Salonen et al, 1995;Abahusain et al, 1999;Ford et al, 1999;Knekt et al, 1999;Will et al, 1999;Polidori et al, 2000;Montonen et al, 2004b). However, the findings on the relation between antioxidants and glucose metabolism are not consistent (Sanchez-Lugo et al, 1997;Reunanen et al, 1998;Liu et al, 1999).…”

Section: Discussionmentioning

confidence: 99%

Food consumption and the incidence of type II diabetes mellitus

et al. 2005

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Objective: The consumption of different foods was studied for their ability to predict type II diabetes mellitus. Design: The study design was a cohort study, based on the Finnish Mobile Clinic Health Examination Survey. Setting: A total of 30 communities from different parts of Finland. Subjects: A total of 4304 men and women, 40-69 y of age and free of diabetes at baseline in 1967-1972 and followed up for incidence of diabetes medication during 23 y (383 incident cases). Results: Higher intakes of green vegetables, fruit and berries, oil and margarine, and poultry were found to predict a reduced risk of type II diabetes. The relative risks of developing type II diabetes between the extreme quartiles of the intakes were 0.69 (95% confidence interval (CI) ¼ 0.50-0.93; P for trend (P) ¼ 0.02) for green vegetables, 0.69 (CI ¼ 0.51-0.92; P ¼ 0.03) for fruit and berries, 0.71 (CI ¼ 0.52-0.98; P ¼ 0.01) for margarine and oil, and 0.71 (CI ¼ 0.54-0.94; P ¼ 0.01) for poultry. Conclusion:The results suggest that prevention of type II diabetes might be aided by consumption of certain foods that are rich in nutrients with hypothesized health benefits.

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Estimation of dehydroascorbic acid in blood of diabetic patients

Cited by 71 publications

References 12 publications

Glucose Transporter Isoforms GLUT1 and GLUT3 Transport Dehydroascorbic Acid

Glucose Transporter Isoforms GLUT1 and GLUT3 Transport Dehydroascorbic Acid

Are Diabetic Neuropathy, Retinopathy and Nephropathy Caused by Hyperglycemic Exclusion of Dehydroascorbate Uptake by Glucose Transporters?

Food consumption and the incidence of type II diabetes mellitus

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