Estimation of conversion factors for fungal biomass determination in compost using ergosterol and PLFA 18:2ω6,9

Klamer, Morten; Bååth, Erland

doi:10.1016/j.soilbio.2003.08.019

Cited by 232 publications

(154 citation statements)

References 54 publications

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“…Carbon use efficiencies of litter and soil-derived C were estimated by the metabolic quotient (qCO 2 ), or the ratio of the respiration rate per unit biomass (Dilly and Munch, 1998). Microbial biomass was measured using PLFA yields converted to mg biomass-C with a conversion factor of 11.8 nmoles PLFA per mg biomass-C for fungi (Klamer and Bååth, 2004) and 363.6 nmol PLFA per mg biomass-C for bacteria (Frostegård and Bååth, 1996). The respiration rate during the 24 h preceding destructive harvesting of the particular sample measured for PLFA was used as the respiration rate.…”

Section: Calculationsmentioning

confidence: 99%

Microbial community structure mediates response of soil C decomposition to litter addition and warming

Creamer

Menezes

Krull

et al. 2015

Soil Biology and Biochemistry

197

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Section: Calculationsmentioning

confidence: 99%

Microbial community structure mediates response of soil C decomposition to litter addition and warming

Creamer

Menezes

Krull

et al. 2015

Soil Biology and Biochemistry

197

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“…This increase in microbial biomass indicated that vermicomposting facilitates microbial proliferation in final stabilized product. Ergosterol content of organic substrates was multiplied by conversion factor 5.4 (Klamer and Baath, 2004) to calculate fungal biomass (FB) in it. Though earthworms selectively consume fungi as their food, increased fungal biomass during vermicomposting suggested that not all the fungi were killed during passage through earthworm guts, in fact the rate of germination of fungal spores was probably enhanced under favourable condition of earthworm guts (Hendrikson, 1990).…”

Section: Discussionmentioning

confidence: 99%

Changes in Fungal and Bacterial Diversity During Vermicomposting of Industrial Sludge and Poultry Manure Mixture: Detecting the Mechanism of Plant Growth Promotion by Vermicompost

Pramanik¹,

Kim²,

Kim³

2011

Biomass - Detection, Production and Usage

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“…15:0, br16:0, 16:1x9, 16:1x7, i17:0, a17:0, cy17:0, 17:0, 10Me17:0, br18:0, 18:1x7 and cy19:0 using the conversion factor of 1.4 Â 10 À17 mol bactPLFA/ cell (Frostegård and Bååth, 1996) and 20 fg biomassC/cell (Bååth, 1994) to calculate bacterial carbon. Fungal biomass was calculated using the conversion factor 11.8 lmol 18:2x6,9/g biomass-C (Klamer and Bååth, 2004).…”

Section: Examination Of the Microbial Communitiesmentioning

confidence: 99%

Variable effects of labile carbon on the carbon use of different microbial groups in black slate degradation

Seifert¹,

Trumbore²,

Xu³

et al. 2011

Geochimica et Cosmochimica Acta

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Weathering of ancient organic matter contributes significantly to biogeochemical carbon cycles over geological times. The principle role of microorganisms in this process is well recognized. However, information is lacking on the contribution of individual groups of microorganisms and on the effect of labile carbon sources to the degradation process. Therefore, we investigated the contribution of fungi, Gram-positive and Gram-negative bacteria in the degradation process using a column experiment. Investigations were performed on low metamorphic black slates. All columns contained freshly crushed, sieved (0.63-2 mm), not autoclaved black slates. Two columns were inoculated with the lignite-degrading fungus Schizophyllum commune and received a culture medium containing 13 C labeled glucose, two columns received only this culture medium and two control columns received only water.The total mass balance was calculated from all carbon added to the slate and the CO 2 and DOC losses. Phospholipid fatty acids (PLFA) were extracted to investigate microbial communities. We used both the compound specific 14 C and 13 C signal of the PLFA to quantify carbon uptake from black slates and the glucose of the culture medium, respectively.The total carbon loss in these columns exceeded the amount of added carbon by approximately 60%, indicating that black slate carbon has been used. PLFA associated with Gram-positive bacteria dominated the indigenous community and took up 22% of carbon from black slate carbon, whereas PLFA of Gram-negative bacteria used only 8% of carbon from the slates. PLFA of Gram-negative bacteria and fungi were both mostly activated by the glucose addition. The added Schizophyllum did not establish well in the columns and was overgrown by the indigenous microbial community. Our results suggest that especially Gram-positive bacteria are able to live on and degrade black slate material. They also benefit from easy degradable carbon from the nutrient broth. In natural environments priming due to root exudates might consequently enhance weathering.

show abstract

Estimation of conversion factors for fungal biomass determination in compost using ergosterol and PLFA 18:2ω6,9

Cited by 232 publications

References 54 publications

Microbial community structure mediates response of soil C decomposition to litter addition and warming

Microbial community structure mediates response of soil C decomposition to litter addition and warming

Changes in Fungal and Bacterial Diversity During Vermicomposting of Industrial Sludge and Poultry Manure Mixture: Detecting the Mechanism of Plant Growth Promotion by Vermicompost

Variable effects of labile carbon on the carbon use of different microbial groups in black slate degradation

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