Estimation of blood acetaldehyde during ethanol metabolism: A sensitive HPLC/fluorescence microassay with negligible artifactual interference

Ung-Chhun, Neng S.; Collins, Michael A.

doi:10.1016/0741-8329(87)90088-7

Cited by 29 publications

(5 citation statements)

References 8 publications

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“…Blood acetaldehyde concentration was determined using an established HPLC method (49). Briefly, blood samples (50 μL) from the retroorbital sinus were collected, 45 min after ethanol ingestion, into heparinized tubes containing 10% perchloric acid.…”

Section: Methodsmentioning

confidence: 99%

Pharmacological recruitment of aldehyde dehydrogenase 3A1 (ALDH3A1) to assist ALDH2 in acetaldehyde and ethanol metabolism in vivo

Chen

Cruz

Mochly‐Rosen

2015

Proc. Natl. Acad. Sci. U.S.A.

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Correcting a genetic mutation that leads to a loss of function has been a challenge. One such mutation is in aldehyde dehydrogenase 2 (ALDH2), denoted ALDH2*2. This mutation is present in ∼0.6 billion East Asians and results in accumulation of toxic acetaldehyde after consumption of ethanol. To temporarily increase metabolism of acetaldehyde in vivo, we describe an approach in which a pharmacologic agent recruited another ALDH to metabolize acetaldehyde. We focused on ALDH3A1, which is enriched in the upper aerodigestive track, and identified Alda-89 as a small molecule that enables ALDH3A1 to metabolize acetaldehyde. When given together with the ALDH2-specific activator, Alda-1, Alda-89 reduced acetaldehyde-induced behavioral impairment by causing a rapid reduction in blood ethanol and acetaldehyde levels after acute ethanol intoxication in both wild-type and ALDH2-deficient, ALDH2*1/*2, heterozygotic knock-in mice. The use of a pharmacologic agent to recruit an enzyme to metabolize a substrate that it usually does not metabolize may represent a novel means to temporarily increase elimination of toxic agents in vivo.

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Section: Methodsmentioning

confidence: 99%

Pharmacological recruitment of aldehyde dehydrogenase 3A1 (ALDH3A1) to assist ALDH2 in acetaldehyde and ethanol metabolism in vivo

Chen

Cruz

Mochly‐Rosen

2015

Proc. Natl. Acad. Sci. U.S.A.

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“…Ethanol concentration was determined by headspace gas chromatography as described previously (Peng et al., ). Acetaldehyde was determined as described by Ung‐Chhun and Collins () with modifications by Peng and colleagues (). Acetate was determined by headspace gas chromatography as described by Giles and colleagues ().…”

Section: Methodsmentioning

confidence: 99%

Steady‐State Metabolism of Ethanol in Perfused Rat Livers Treated with Cyanamide: Quantitative Analysis of Acetaldehyde Effects on the Metabolic Flux Rates

Yao

Lai

Yin

2015

Alcoholism Clin & Exp Res

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“…Acetaldehyde in blood was determined as a fluorescent adduct, formed by reaction with 1,3-cyclohexanedione and ammonium ion, by a high-performance liquid chromatographic method (Peng et al, 1999) modified from Ung-Chhun and Collins (1987).…”

Section: Determination Of Blood Ethanol and Acetaldehydementioning

confidence: 99%

Alcohol Metabolism and Cardiovascular Response in an Alcoholic Patient Homozygous for theALDH2*2Variant Gene Allele

Chen

Peng

et al. 1999

Alcoholism Clin & Exp Res

103

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The gene status of ALDH2*2/*2 alone can tremendously but not completely (as thought previously) protect against development of alcohol dependence. Individuals carrying the combinatorial genotype of ADH2*2/*2-ALDH2*2/*2 are at the least risk for the disease in East Asians. Physiological tolerance or innate insensitivity to the accumulation of blood acetaldehyde following alcohol ingestion may be crucial for the development of alcoholism in individuals homozygous for ALDH2*2.

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Estimation of blood acetaldehyde during ethanol metabolism: A sensitive HPLC/fluorescence microassay with negligible artifactual interference

Cited by 29 publications

References 8 publications

Pharmacological recruitment of aldehyde dehydrogenase 3A1 (ALDH3A1) to assist ALDH2 in acetaldehyde and ethanol metabolism in vivo

Pharmacological recruitment of aldehyde dehydrogenase 3A1 (ALDH3A1) to assist ALDH2 in acetaldehyde and ethanol metabolism in vivo

Steady‐State Metabolism of Ethanol in Perfused Rat Livers Treated with Cyanamide: Quantitative Analysis of Acetaldehyde Effects on the Metabolic Flux Rates

Alcohol Metabolism and Cardiovascular Response in an Alcoholic Patient Homozygous for theALDH2*2Variant Gene Allele

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