Estimation of Ascorbic Acid by Titration

McHenry, E. W.; Graham, Murray

doi:10.1038/135871b0

Cited by 20 publications

(4 citation statements)

References 3 publications

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“…As (19) in this sort of difference evidence for combined and uncombined ascorbic acid whereas others (18,38) PLANT PHYSIOLOGY tion. The salts which do affect respiration (KCl which increases it, and CaCl2 which decreases it) have only a little, and barely significant effect on the final, total ascorbic acid content (compare the data for the KCl and CaC12 series at the same time periods in tables XII and XIII).…”

Section: The Determination Of Ascorbic Acid In Potato Discsmentioning

confidence: 99%

Metabolic Processes of Potato Discs Under Conditions Conducive to Salt Accumulation

Steward

Preston

1940

Plant Physiol.

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IntroductionModern inivestigations (11, 34) show that vital activity plays an essential part in salt acetmulationi by planits. The ability to accumulate2 demands properties peculiar to cells which are still able to grow and the rate at which these are utilized is causally related to the intensity of aerobic respiration. Salt accumulation is not merely the result of equilibria at membrane surfaces but demands some "dynamic machinery" in cells-to use a happy phrase which received prominence in a recent symposium. A similar concept is implied by the oft repeated assertion that during salt accumulation work is done and energy exchanges are involved. The identification of the "dynamic machinery" and the mechanism by which the energy stored in substances of high energy content is ultimately utilized in salt accumulation is one of the most challenging problems of modern plant physiology.The earlier work, done on Nitella, Elodea, discs cut from storage organs, and roots, focussed attention upon those biochemical processes in the accumulating cells which involve carbohydrates and suggested that these represent an important part of the machinery of accumulation. In a brief review of available evidence (33) it was concluded that the biochemistry of salt absorption represented a virgin field for investigation and this inspired much of the work to be described in this and ensuing papers.The relationship between salt accumulation and metabolism has been most investigated with reference to respiration as measured by carbon dioxide output. The conclusion to be drawn (11,33, 34) is that the relation between salt uptake and respiration is not a simple and direct one; it concerns the aerobic phases of the respiratory process but it is the oxidation which these involve, rather than the production of carbon dioxide per se, which is causally concerned in salt accumulation. The ultimate connection between respiration and salt accumulation must therefore involve some aspect of the oxidative breakdown of sugar-the ultimate source of carbon substrates-other than the mere evolution of the carbon dioxide which is finally produced. The energy thus released must be involved; not only because it supplies the relatively small amount of energy necessary for the actual process of salt uptake, (a process which tends to increase the free 'This is the first of a group of papers on the biochemistry of salt absorption. 2 Especially that most general type of accumulation which involves the simultaneous uptake of anion and cation and which has been termed "primary absorption." Certain absorption processes in which metabolism is not involved are not in question here. PLANT PHYSIOLOGY energy of the system concerned) but also because it maintains the cells at that general level of metabolic activity which is an invariable characteristic of systems capable of a high degree of salt accumulation. Hence the biochemistry of salt accumulation entails an account of the fate of the carbohydrates metabolized during salt accumulation-an account which must ultimat...

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Section: The Determination Of Ascorbic Acid In Potato Discsmentioning

confidence: 99%

Metabolic Processes of Potato Discs Under Conditions Conducive to Salt Accumulation

Steward

Preston

1940

Plant Physiol.

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“…Although many have proposed new ways to perform and quantify WB data (Ntoukas et al, 2021;Suzuki, Koura, Noguchi, Uchio-Yamada, & Matsuda, 2011;Taylor, Berkelman, Yadav, & Hammond, 2013), having accurate and precise quantitative measurements is still problematic. Here we describe how the application of the titration assay, originally developed to quantify vitamin C (McHenry & Graham, 1935), leads to accurate and precise quantitative WB results, and we name this method titration-Western Blot (t-WB). We have previously applied titration assay to WB for protein quantification in clinical and preclinical studies (Ahmed et al, 2019;Gnocchi et al, 2020;Minniti et al, 2020;Parini et al, 2008;Pedrelli et al, 2014;Pramfalk et al, 2022;Pramfalk et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

Titration-WB: A methodology for accurate quantitative protein determination overcoming reproducibility errors

Maestri,

Ehrenborg,

Werngren

et al. 2024

Preprint

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Western blot (WB) technique is widely used to identify and quantify proteins in biological samples, but it is poorly accurate and precise for quantitative purposes. Merging the WB with the titration assay, we established a reliable method for protein quantification based on the slope of the linear function for signal-intensity/total protein loaded. Titration-WB unravels systematic and operator errors, compensates for problems in antibody affinity/avidity, and permits trustworthy inter-WB comparison.

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“…Subsequently other workers have also employed this device to allow for the fugitive character of the excess dye coloration. When a Spekker photoelectric absorptiometer was employed for the determination of ascorbic acid in fruits and jams by this method it was found that fading of the colour of excess indophenol was sometimes very rapid, especially with solutions which had been treated with hydrogen sulphide for the estimation of the partially oxidized dehydroascorbic acid [McHenry & Graham, 1935]. Accuracy ofextrapolation in such cases was not high and for this reason it was considered inadvisable to rely on readings of the colour due to a single addition of indophenol.…”

mentioning

confidence: 99%

The application of the Spekker photoelectric absorptiometer to the determination of vitamin C

Taylor¹

1943

Biochemical Journal

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Estimation of Ascorbic Acid by Titration

Cited by 20 publications

References 3 publications

Metabolic Processes of Potato Discs Under Conditions Conducive to Salt Accumulation

Metabolic Processes of Potato Discs Under Conditions Conducive to Salt Accumulation

Titration-WB: A methodology for accurate quantitative protein determination overcoming reproducibility errors

The application of the Spekker photoelectric absorptiometer to the determination of vitamin C

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