Estimation of alpha-amino nitrogen in plasma and urine by the colorimetric ninhydrin reaction

Matthews, D. M.; Muir, G. G.; Baron, D N

doi:10.1136/jcp.17.2.150

Cited by 65 publications

(13 citation statements)

References 6 publications

(2 reference statements)

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“…The protein bands were stained with 0-05 % amido-black 10B (Harleco, Philadelphia) in 7 % acetic acid for 1 h. The cleared gels were photographed and scanned in a recording electrophoresis densitometer (Densicord Photovolt, New York). The concentration of -amino nitrogen in the fluid samples was determined colorimetrically with the ninhydrin reagent as described by Matthews, Muir & Baron (1964). The concentration of urea nitrogen in the fluid samples was determined by the urease method (Fawcett & Scott, 1960) with modification (Chaney & Marbach, 1962).…”

Section: Methodsmentioning

confidence: 99%

Changes in the composition of maternal plasma, fetal plasma and fetal extraembryonic fluid during gestation in the rat

Tam¹,

Chan²

1977

Reproduction

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The changes in the biochemical composition of maternal plasma, fetal plasma and fetal extraembryonic fluid were studied in Wistar rats from Days 13 1/2 to 21 1/2 of gestation. During this period, there was an increase in maternal plasma volume and total blood volume. The total protein concentration decreased, but little change was observed in the protein pattern, total alpha-amino nitrogen, urea nitrogen and sodium concentration. In fetal plasma, total protein concentration increased progressively with a prominent increase in albumin near term. The albumin:globulin ratio was lower than that in maternal plasma because of the presence of two alpha-fetoproteins and four transferrins. The concentration of alpha-amino nitrogen was higher than that in maternal plasma but the urea nitrogen level did not change significantly. The fetal extraembryonic fluid reflected the changes in composition of fetal plasma, especially in the protein pattern, and concentrations of alpha-amino nitrogen and sodium. Total protein and urea nitrogen increased markedly in concentration near term but there was reduction in the fluid volume at this time.

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Section: Methodsmentioning

confidence: 99%

Changes in the composition of maternal plasma, fetal plasma and fetal extraembryonic fluid during gestation in the rat

Tam¹,

Chan²

1977

Reproduction

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“…Serum level of a-amino acids was measured according to Matthews et al (1964). Serum total lipid was estimated as described by Woodman & Price (1972).…”

Section: Determination Of Serum Ions and Metabolitesmentioning

confidence: 99%

Influence of oral administration of 3,5,3′‐triiodo‐thyronine on growth, digestion, food conversion and metabolism in the underyearling red sea bream, Chrysophrys major (Temminck & Schlegel)

Woo

Chung

1991

Journal of Fish Biology

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The effect of inclusion of 3,5,3'-triiodo-~-thyronine (T,) in the diet was examined in underyearling red sea bream Chrysophrys major (Temminck 8c Schlegel). The treatment brought about increases in growth rate, appetite, food conversion efficiency and activities of intestinal enzymes including leucine nitroanilidase, alkaline phosphatase, y-glutamyltransferas, a-amylase and disaccharidase. There were no changes in the muscle content of water, protein, lipid and glycogen. Liver glycogen content was elevated, as well as activities of the hepatic enzymes glycogen phosphorylase, glycogen synthetase, glutamate-pyruvate transaminase, fructose-1.6-diphosphatase and glucose-6-phosphatase. The serum concentrations of total protein, albumin, globulin, u-amino acids. glucose, ammonia and calcium were increased by the treatment whereas the serum concentrations of free fatty acids, cholesterol and triglyceride remained unaltered. The results suggest that in the red sea bream T, stimulated protein and carbohydrate but not lipid metabolism and that the hormone promoted growth by improving appetite, digestion and absorption.

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“…Serum albumin and globulin concentrations were measured according to Sigma Bulletins 630 and 560 respectively. Serum level of (-amino acids was measured according to Matthews et al (1964). Serum ammonia was estimated using glutamate dehydrogenase according to Sigma Bulletin 170-A.…”

Section: Determination Of Serum Ions and Metabolitesmentioning

confidence: 99%

Influence of oral administration of estradiol-17? and testosterone on growth, digestion, food conversion and metabolism in the underyearling red sea bream, Chrysophrys major

Woo

Chung

1993

Fish Physiol Biochem

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Estradiol-17β (E2) administered in the diet to the red sea bream Chrysophrys major did not affect appetite, food conversion efficiency, protein efficiency ratio and specific growth rate. Serum concentrations of total protein, albumin, globulin, vitellogenin, α-amino acids, total lipid, free fatty acids, cholesterol and calcium were elevated. The hepatosomatic index was also increased. Activities of hepatic enzymes including lactate dehydrogenase, isocitrate dehydrogenase and glutamate-pyruvate transaminase were higher than found in untreated control fish. Intestinal activity of leucine aminopeptidase was augmented. However, there were no changes in muscle water, protein, lipid and glycogen content. In contrast, testosterone (T) given by the same route increased appetite, food conversion efficiency, protein efficiency ratio and specific growth rate. There were no alterations in serum protein and calcium concentrations but serum glucose, ammonia and triglyceride levels were elevated. Hepatic glycogen content was increased. The activities of hepatic fructose- 1,6-diphosphatase, glucose-6-phosphatase and glycogen synthetase and intestinal activities of alkaline phosphatase and τ-glutamyltransferase were higher than noted on control fish. The results reveal that estradiol-17β and testosterone exerted different metabolic effects in the red sea bream and they suggest that testosterone exerts its anabolic actions by increasing appetite, food conversion efficiency and activities of digestive enzymes.

show abstract

Estimation of alpha-amino nitrogen in plasma and urine by the colorimetric ninhydrin reaction

Cited by 65 publications

References 6 publications

Changes in the composition of maternal plasma, fetal plasma and fetal extraembryonic fluid during gestation in the rat

Changes in the composition of maternal plasma, fetal plasma and fetal extraembryonic fluid during gestation in the rat

Influence of oral administration of 3,5,3′‐triiodo‐thyronine on growth, digestion, food conversion and metabolism in the underyearling red sea bream, Chrysophrys major (Temminck & Schlegel)

Influence of oral administration of estradiol-17? and testosterone on growth, digestion, food conversion and metabolism in the underyearling red sea bream, Chrysophrys major

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