Estimation of Abamectin Residues Present in Tea: High-Performance Liquid Chromatography Technique

Abstract

A simple, reliable, and sensitive method was based on high-performance liquid chromatography (HPLC) was developed and validated for the estimation of abamectin residues present in tea. The abamectin residues extracted with acetone-water mixture (70 : 30, v/v) and derivatised with 1-methylimidazole (1-MIM) and trifluoroacetic anhydride (TFAA) were estimated by HPLC using fluorescence detector (FLD). The technique was validated in terms of linearity, precision, recovery, specificity, limit of detection (LOD), and limit of quantification (LOQ). A good linear relationship ( 2 > 0.99) was absorbed in the abamectin concentration range from 0.01 to 1.0 g mL −1 . The limit of detection and limit of quantification of the method were 0.01 and 0.02 g g −1 , respectively. The average recoveries of the pesticide from black tea and dried green leaves ranged from 83.3 to 103.8% and 83.8 to 98.0%, respectively.