Estimation and characterization of soluble fibrin monomer complexes during endotoxin-induced intravascular coagulation

Hafter, R.; Müller‐Berghaus, Gert; Hugo, R. von; Graeff, H.

doi:10.1016/0049-3848(77)90053-6

Cited by 15 publications

(16 citation statements)

References 8 publications

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“…Yet, in the present series of patients with hypercoagulability no covalently linked fibrin derivatives were found. This could well be in agreement with the observations during the generalized Shwartzman reaction that hypercoagulability (before the second endotoxin injection) was accompanied by an elevated level of SFMC dissociable in SDS and urea, whereas intravascular coagulation (4 h after the second endotoxin injection) was evidenced by the occurrence of SDS-and urearesistant oligomers of fibrin containing y-y dimers [14].…”

Section: Discussionsupporting

confidence: 80%

Evaluation of Hypercoagulability in Septic Abortion

Graeff¹,

Ernst²,

Bocaz³

et al. 1976

Pathophysiol Haemos Thromb

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Soluble fibrin monomer complexes (SFMC) were determined in patients with septic abortion (body temperatures of more than 39 °C and/or chills without apparent signs of endotoxic shock), with infected abortion, with non-infected abortion and with normal pregnancies. Quantitative gel filtration (4% agarose) of β-alanine precipitated plasma samples yielded the relative (percent of total fibrinogen content) and absolute (mg/l00 ml plasma) amount of SFMC. The relative (5.5 ± 1.4%, mean ± SD) and absolute (21.5 ± 8.6 mg/l00 ml) amount of SFMC was significantly increased in patients with septic abortion compared to patients with normal pregnancies or non-infected abortion (p < 0.001). Patients with infected abortion already revealed increased levels of SFMC (4.3 ± 1.2%, 14.2 ± 6.8 mg/l00 ml) though their platelet count was still unaltered (infected abortion: 221 ± 47 × 10³ platelets/mm³; septic abortion: 99 ± 36 × 10³ platelets/mm³). The use of heparin in patients with septic abortion resulted in a decrease in SFMC. Chain characterization of SFMC frequently revealed a slight degradation of the α-chains probably due to fibrinolytic activity in vivo; γ-γ dimers representing inter-molecular covalent bindings were not observed. The findings are in agreement with our former assumption that patients with septic abortion have a pronounced state of hypercoagulability.

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Section: Discussionsupporting

confidence: 80%

Evaluation of Hypercoagulability in Septic Abortion

Graeff¹,

Ernst²,

Bocaz³

et al. 1976

Pathophysiol Haemos Thromb

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“…In some reports of experimental DIC in animals, fibrin deposits have been histologically observed in the capillaries of different organs, demonstrating that fibrin deposition is one of the consequences of DIC. 6,7,11,12 During DIC, the massive fibrin deposition that occurs in the microcirculation may occlude or restrict blood flow, and the resulting ischemia may lead to necrosis and potentially MOF. [31][32][33] DIC is thought to be a common complication in horses with colic, especially in those with ischemic and inflammatory processes.…”

Section: Discussionmentioning

confidence: 99%

“…[6][7][8][9][10] Other histologic studies in people with DIC have shown that excessive production and massive deposition of fibrin can be observed in capillaries from several organs, such as the kidneys, lungs, liver, spleen, heart, and brain. 6,7,11,12 The organs most frequently affected by microthrombi deposition in patients with DIC are lungs, kidneys, and liver. 4,7,9,13,14 In veterinary medicine, the detection of microvascular fibrin deposition in 1 or more organs also is considered essential for a histopathologic diagnosis of DIC.…”

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confidence: 99%

Detection of Fibrin Deposits in Tissues from Horses with Severe Gastrointestinal Disorders

Cotovio¹,

Monreal²,

Navarro³

et al. 2007

J Vet Int Med

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Background: In humans and experimental animals, disseminated intravascular coagulation (DIC) causes fibrin deposition in several organs, which eventually leads to ischemia and multiorgan failure.Hypothesis: Horses who died or were euthanized for severe gastrointestinal disorders could have fibrin deposits in different tissues.Animals: Tissue-organ samples collected during postmortem examinations on 66 colic horses with poor prognoses (eg, severe intestinal ischemia, enteritis, peritonitis), from 11 colic horses with good prognoses (eg, large-colon obstruction or displacement), and from 16 slaughter horses.Methods: Tissue samples (kidney, lung, liver) were stained with hematoxylin and eosin, and phosphotungstic acid hematoxylin for a blinded histologic examination. A fibrin score (grades 0 to 4) was established for each tissue sample and for each horse.Results: Fibrin deposits were found in tissue specimens of 11 of 27 of horses (40.7%) in the ischemic group, 8 of 21 in the enteritis group (38.1%), and 7 of 18 in the peritonitis group (39.0%), whereas none of the horses in the obstructive group (n 5 11) and only 1 horse in the slaughter group (n 5 16) had fibrin deposits in their tissues. In addition, the mean fibrin score values for the ischemic, enteritis, and peritonitis groups (1.3 6 1.7, 1.1 6 1.6, and 0.9 6 1.3, respectively) were statistically higher than those for the obstructive and slaughter groups (0.0 6 0.0 and 0.1 6 0.5, respectively). The largest fibrin deposits were found in the lungs.Conclusions and Clinical Importance: Horses with severe gastrointestinal disorders have fibrin deposits that are consistent with capillary microthrombosis, multiorgan failure, and DIC.

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“…As a result of the increased thrombin action on fibrinogen, fibrin monomer and crosslinked fibrin is formed in the circulation [13]. Polymerisation of fibrin monomer is inhibited to a certain extent by complex formation with fibrinogen and fibrin degradation products [10,20] resulting in the so called soluble fibrin monomer complexes (SFMC).…”

Section: Pathological Findingsmentioning

confidence: 99%