Abstract:Substrate and product concentration data obtained by simulating enzyme-substrate reaction rate equations were used to test two proposed kinetic rate constant estimation techniques in this study. In the first technique, the turnover number, k 3 , was calculated using early transient time domain data, which are difficult to obtain experimentally. The technique used an iterative approach to calculate k 3 with a pair of data and the value of k 3 could be retrieved with 35% error. The second technique calculated k … Show more
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