Estimating the relative avidity of mucosal IgA for antigen

Jones, Colin L.; Macdonald, Ross A.; Hosking, C. S.; Roberton, Don

doi:10.1016/0022-1759(87)90420-0

Cited by 27 publications

(16 citation statements)

References 7 publications

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“…IgA antibodies of EmA-negative patients could not be removed from the gut tissue by moderate amounts of KSCN. As KSCN is often used to test the avidity of antigen-antibody binding, 24 our results also indicate that coeliac antibodies are bound to intestinal TG2 with considerably high avidity. During a longstanding immune reaction, antibodies with increasing avidity are produced, which makes it understandable why older patients with coeliac disease may have lower serum EmA levels than the younger ones.…”

Section: Discussionsupporting

confidence: 62%

See 1 more Smart Citation

Endomysial antibody-negative coeliac disease: clinical characteristics and intestinal autoantibody deposits

Salmi

Collin

Korponay-Szabó

et al. 2006

Gut

227

163

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Section: Discussionsupporting

confidence: 62%

“…24 After further washing in PBS, the sections were stained for human IgA and TG2 as described in the previous section.…”

Section: Small-bowel Mucosal Tg2-targeted Iga Depositsmentioning

confidence: 99%

Endomysial antibody-negative coeliac disease: clinical characteristics and intestinal autoantibody deposits

Salmi

Collin

Korponay-Szabó

et al. 2006

Gut

227

163

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“…The avidity of serum IgG antibodies was determined using potassium thiocyanate (KSCN) to the elution of the bound complexes, as described previously elsewhere [15], with some modifications. PGL-1 coated microplates were incubated with 1:50 dilution of serum samples (only those with positive results for anti-PGL-1 IgG were tested) during 24 h at 4 o C. After washing, 50 µL of 2 M KSCN were added to the plates.…”

Section: Serum Anti-pgl-1 Igg Aviditymentioning

confidence: 99%

Salivary anti-PGL IgM and IgA titers and serum antibody IgG titers and avidities in leprosy patients and their correlation with time of infection and antigen exposure

Nagão-Dias¹,

Almeida²,

Oliveira³

et al. 2007

Braz J Infect Dis

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The present work proposed to correlate serum antibody avidity and salivary antibody titers as parameters for time of infection and antigen exposure in a co-hort study evaluating leprosy patients in different periods of treatment. Colorimetric enzyme-immunoassays for salivary antibodies, serum antibody IgG titers and avidities were performed in the samples. Anti-PGL-1 IgA and IgM salivary antibodies were significantly higher in multibacillar (MB-L) patients compared to normal controls (p<0.05), but not when compared to borderline tuberculoid (BT) or to paucibacillar (PB-L) patients (p>0.05). A good correlation was found between salivary anti-PGL-1 IgA and IgM levels in MB-L patients (r=0.41, p<0.01). Two out of 33 tested saliva samples from patients who had completed the drug regimen treatment presented positive salivary antibodies. Among non-treated patients, samples with low, medium or high serum IgG antibody avidity were found in similar frequencies. In patients under treatment, most of the serum samples showed low or medium IgG antibody avidity. The treated MB-L patients showed medium or high antibody avidity, except for two, who showed very low antibody avidity results. We suggest that salivary anti-PGL antibodies and serum IgG avidity could be useful for the indication of recent exposure or re-exposure to bacteria after chemotherapy.

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“…The avidities of the anti- Sa IgG and IgA antibodies in the maternal and umbilical cord sera and in the maternal colostrum samples were determined by elution with different molarities (M) of potassium thiocyanate (0.0–4.0 M) according to the technique described by Jones et al 14. The results were expressed as the molarity of potassium thiocyanate necessary to elute 50% of the bound antigen-antibody complexes.…”

Section: Methodsmentioning

confidence: 99%

Passive acquisition of anti-Staphylococcus aureus antibodies by newborns via transplacental transfer and breastfeeding, regardless of maternal colonization

Nadaf¹,

Lima²,

Stranieri³

et al. 2016

Clinics

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OBJECTIVE:To investigate the transmission of anti-Staphylococcus aureus (Sa) IgG, IgG1 and IgG2 via placental transfer and the transfer of IgA via the colostrum according to maternal Sa carrier status at delivery.METHODS:We evaluated anti-Sa IgG, IgG1 and IgG2 in maternal and cord sera and IgA in colostrum from a case (n=49, Sa+) and a control group (n=98, Sa-).RESULTS:Of the 250 parturients analyzed for this study, 49 were nasally colonized with S. aureus (prevalence of 19.6%). Ninety-eight non-colonized subjects were selected for the control group. The anti-Sa IgG, IgG1 and IgG2 levels and the IgG avidity indexes in the maternal and cord sera did not differ between the groups, with a low transfer ratio of anti-Sa IgG to the newborns in both groups. The anti-Sa IgG2 titers were significantly higher than the IgG1 titers in the maternal and cord sera. Inversely, the transfer ratios were higher for anti-Sa IgG1 compared with IgG2; however, no differences between the groups were detected. The Sa-specific IgA levels and avidity indexes in the colostrum were equivalent between groups.CONCLUSIONS:Maternal Sa nasal colonization at delivery is not associated with higher antibody levels in the mother or newborns. The high titers of anti-Sa IgG2 found in the cord serum indicate a greater reactivity with non-protein antigens, which may further contribute to the susceptibility to staphylococcal infections at birth. The presence of IgA in the colostrum with avidity to S. aureus reinforces the importance of breastfeeding shortly after birth.

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Estimating the relative avidity of mucosal IgA for antigen

Cited by 27 publications

References 7 publications

Endomysial antibody-negative coeliac disease: clinical characteristics and intestinal autoantibody deposits

Endomysial antibody-negative coeliac disease: clinical characteristics and intestinal autoantibody deposits

Salivary anti-PGL IgM and IgA titers and serum antibody IgG titers and avidities in leprosy patients and their correlation with time of infection and antigen exposure

Passive acquisition of anti-Staphylococcus aureus antibodies by newborns via transplacental transfer and breastfeeding, regardless of maternal colonization

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