Estimating the environmental impact of micro-pollutants in the low Ebro River (Spain): An approach based on screening toxicity with Vibrio fischeri

Ocampo-Duque, William; Sierra, Jordi; Ferré-Huguet, Núria; Schuhmacher, Marta; Domingo, José L.

doi:10.1016/j.chemosphere.2008.03.055

Cited by 35 publications

(30 citation statements)

References 23 publications

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“…The EC50 values of sediments in this study were comparable to or lower than those in the following regions: Lake Geneva, USA (0.008-3.42 mg/mL) (Pardos et al, 1999), Southern Puget Sound, USA (0.31-175.30 mg/mL) (Long et al, 2002), Waukegan Harbor, USA (0.42-14.50 mg/mL) (Kemble et al, 2000), Ebro River, Spain (0.04-12.96 mg/mL) (Ocampo-Duque et al, 2008), Po River, Italy (0.46-36.80 mg/mL) (Viganò et al, 2003), and Irish marine sediments (>1,000 mg/mL) (Macken et al, 2008). However, our values were much higher than those in Barcelona Harbor, Spain (0.0002-0.0044 mg/mL) (Martínez-Lladó et al, 2007), which is associated with intensive marine and industrial activities.…”

Section: Discussionmentioning

confidence: 99%

“…It is sensitive, reproducible, cost effective, and simple to conduct. Furthermore, it does not entail ethical problems, and requires only 5-30 min for toxicity prediction (Park et al, 2005;Parvez et al, 2006;Ocampo-Duque et al, 2008). Its results have been compared with those of other conventional bioassays, with good correlation in many cases (Guerra, 2001;Abbondanzi et al, 2003;Parvez et al, 2006).…”

Section: Introductionmentioning

confidence: 95%

“…For inferring probable adverse biological effects, biological analyses must be combined with the chemical analyses. A combined ecotoxicological and chemical approach can significantly improve the reliability of assessment of the environmental health (Ocampo-Duque et al, 2008;Macken et al, 2008;Narracci et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

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Sediment Toxicity of Industrialized Coastal Areas of Korea Using Bioluminescent Marine Bacteria

Choi¹,

Kim²,

Yoon³

et al. 2010

Fisheries and aquatic sciences

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The quality of marine sediments from the industrialized coastal areas of Korea (Ulsan Bay, Masan Bay, and artificial Lake Shihwa) was investigated using a bacterial bioluminescence toxicity test. Sediment toxicity results were compared with the levels of chemical contamination (trace metals, organic wastewater markers, acid volatile sulfides, total organic carbon). Effective concentration 50% (EC50) of sediments ranged from 0.014 to 1.126 mg/mL, which is comparable to or lower than values in contaminated lakes, rivers, and marine sediments of other countries. Sediment reference index (SRI) ranged from 13 to 1044, based on the EC50 of the negative control sample. Mean average SRI values in Masan Bay and Lake Shihwa were approximately 8 and 9 times as high as that in Ulsan Bay, indicating higher sediment toxicity and greater contamination in the two former regions. Sediment toxicity were strongly associated with the concentrations of some chemicals, suggesting that this test may be useful for determining potential chemical contamination in sediments.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 95%

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Sediment Toxicity of Industrialized Coastal Areas of Korea Using Bioluminescent Marine Bacteria

Choi¹,

Kim²,

Yoon³

et al. 2010

Fisheries and aquatic sciences

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“…This is based on the inhibition of bioluminescent bacterium, Vibrio fischeri (Brack 2003;Gabrielson et al 2003;Parvez et al 2006;Farre et al 2007;Greenwood et al 2007;Wadhia and Thompson 2007;Ocampo-Duque et al 2008). Short-term tests with V. fischeri detect effects on bacterial energy metabolism and are very sensitive to a range of toxicants and environmental samples (Parvez et al 2006;Ocampo-Duque et al 2008). However, although it detects log K OW -dependent general narcosis, many ecotoxicologically relevant specific effects (e.g.…”

Section: Introductionmentioning

confidence: 99%

An assessment of the potential of the microbial assay for risk assessment (MARA) for ecotoxicological testing

Fai

Grant

2010

Ecotoxicology

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Rapid microscale toxicity tests make it possible to screen large numbers of compounds and greatly simplify toxicity identification evaluation and other effect directed chemical analyses of effluents or environmental samples. Tests using Vibrio fischeri (such as Microtox®) detect toxicants that cause non-specific narcosis, but are insensitive to other important classes of contaminants. The microbial assay for risk assessment (MARA) is a 24 h multi-species test that seeks to address this problem by using a battery of ten bacteria and a fungus. But there has been little independent evaluation of this test, and there is no published information on its sensitivity to pesticides. Here, we assess the performance of MARA using a range of toxicants including reference chemicals, fungicides and environmental samples. Mean MARA microbial toxic concentrations and IC(20)s (20% Inhibitory concentrations) indicate the toxicant concentrations affecting the more sensitive micro-organisms, while the mean IC(50) (50% Inhibitory concentration) was found to be the concentration that was toxic to most MARA species. For the two fungicides tested, the yeast (Pichia anomalia) was the most sensitive of the ten MARA species, and was more sensitive than the nine other yeasts tested. The test may be particularly valuable for work with fungicides. Mean MARA IC(50)s were comparable to values for nine other yeast species and the lowest individual IC(50)s for each toxicant were comparable to reported IC(50)s for Daphnia magna, Selenastrum capricornutum and Microtox® bioassays. MARA organisms exhibited more variable sensitivities, with the most sensitive organism being different for different samples, enhancing the likelihood of toxicity detection and giving a toxicity "fingerprint" that may help identify toxicants. The test, therefore, has great potential and would be valuable for ecotoxicological testing of pollutants.

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“…The EC 50 values of sediments in this study were much higher than those reported in the following Korean coastal regions associated with intensive marine and industrial activities: Masan Bay (0.014-0.089 mg/mL), Lake Shihwa (0.018-0.061 mg/mL), Ulsan Bay (0.066-1.126 mg/mL) , and the outfall area of the Masan WWTP (0.014-0.148 mg/mL) (Choi et al, 2010c). However, Southern Puget Sound, USA (0.31-175.30 mg/mL) (Long et al, 2002), the Po River, Italy (0.46-36.80 mg/mL) (Viganò et al, 2003), Waukegan Harbor, USA (0.42-14.50 mg/mL) (Kemble et al, 2000), and the Ebro River, Spain (0.04-12.96 mg/mL) (Ocampo-Duque et al, 2008) were higher or comparable to our results. Therefore, sediment toxicity in the present study is considered within the middle range of toxicities reported worldwide.…”

Section: Sediment Toxicity Using Vibrio Fischerimentioning

confidence: 99%

Chemical Contamination and Toxicity of Sediments from the Gunsan Coast, Korea

Lee¹,

Choi²,

Hwang³

et al. 2012

Fisheries and aquatic sciences

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Polycyclic aromatic hydrocarbons (PAHs), butyltins (BTs), nonylphenol (NP), and fecal sterols concentrations in sediments were investigated from Gunsan coast of Korea to evaluate organic pollution from anthropogenic activities. Sediment toxicity was also examined by bacterial bioluminescence toxicity test (Vibrio fischeri). The concentrations of 16 PAHs in sediments ranged from 67.9 to 425 ng/g dry wt; BTs ranged from 2.79 to 14.1 ng Sn/g dry wt; NP ranged from 20.7 to 2171 ng/g dry wt; and coprostanol, a fecal sterol, ranged from 7.60 to 245 ng/g dry wt. Effective concentration 50% (EC 50 ) of sediments ranged from 0.38 to 23.8 mg/ mL. Most of the chemicals were present at levels lower than or comparable to the previously reported values from Korea. However, NP levels in the present study were in the high range of levels reported from the Korean coast, and 40% of the measured samples exceeded screening and ecotoxicological values of NP suggested by the Netherlands and Canada. This suggests that an ongoing source of NP is a serious concern in the Gunsan coast. High levels of contaminants were found in the proximity of potential sources, such as the outfall of a wastewater treatment plant for NP, an anthracite-fired power plant for PAHs, and ports for BTs, fecal sterols, and sediment toxicity. This indicates that Gunsan coast has various potential sources of marine sediment contaminants.

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Estimating the environmental impact of micro-pollutants in the low Ebro River (Spain): An approach based on screening toxicity with Vibrio fischeri

Cited by 35 publications

References 23 publications

Sediment Toxicity of Industrialized Coastal Areas of Korea Using Bioluminescent Marine Bacteria

Sediment Toxicity of Industrialized Coastal Areas of Korea Using Bioluminescent Marine Bacteria

An assessment of the potential of the microbial assay for risk assessment (MARA) for ecotoxicological testing

Chemical Contamination and Toxicity of Sediments from the Gunsan Coast, Korea

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