Estimating the biodegradation of pesticide in soils by monitoring pesticide-degrading gene expression

Monard, Cécile; Martin-Laurent, Fabrice; Lima, Oscar; Devers‐Lamrani, Marion; Binet, Françoise

doi:10.1007/s10532-012-9574-5

Cited by 31 publications

(31 citation statements)

References 56 publications

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“…For example, in the case of diuron, Pesce et al (2013) recently demonstrated that diuron mineralization kinetics obtained with soil samples were correlated to diuron-degrading genetic potentials estimated from the quantification of the abundance of the phenlylurea hydrolase gene puhB, confirming that degradation genes could be potential biomarkers for the detection of organic xenobiotics (Sipilä et al, 2008). In addition, Monard et al (2013) recently showed that atrazine mineralizing ability of different soils was correlated to the expression level of atzD gene, coding for an enzyme responsible for s-triazine ring opening. However, one prerequisite for applying such approaches is knowledge of the degradation pathway and of the genes coding degrading enzymes.…”

Section: Did the Ban Of Diuron Lead To A Decrease In The Exposure Levmentioning

confidence: 92%

Freshwater sediment pesticide biodegradation potential as an ecological indicator of microbial recovery following a decrease in chronic pesticide exposure: A case study with the herbicide diuron

Pesce¹,

Margoum²,

Rouard³

et al. 2013

Ecological Indicators

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The aim of this study was to evaluate the use of freshwater sediment biodegradation potential as an ecological indicator for monitoring microbial recovery following a decrease in chronic pesticide exposure. For this purpose, a four-year case study (2008)(2009)(2010)(2011) was conducted in a small stream (Morcille river) long exposed to high diuron concentrations, increasing from upstream to downstream. Our results show that the ban on diuron in December 2008 resulted in a progressive decrease in its concentrations in the Morcille river over the survey period. However, diuron remained present in the water three years after the ban. The spatio-temporal variations in the sediment biodegradation potential were assessed by radiorespirometry using [ring-U-14C] diuron to estimate diuron mineralization potentials. Between autumn 2008 and autumn 2011, mean diuron mineralization percentage after 15 weeks of incubation decreased by 65% downstream and by 82% in the intermediate sector, and mean 10% diuron dissipation time values increased between 143% (downstream) and 210% (intermediate). Thus the decrease in the level of chronic diuron exposure in the river also caused a fall in sediment diuron-mineralizing capacities, revealing a corresponding recovery of microbial communities. Our results show that the use of freshwater sediment biodegradation potential may be useful for assessing microbial recovery after a decrease in chronic exposure to pollutants, opening prospects for developing a new class of ecological indicator to monitor the recovery of biological quality of water resources. In this way, the use of molecular approaches based on direct extraction of nucleic acids from environmental matrices and their subsequent analysis by PCR-based approaches to quantify the abundance of pesticide-degrading communities could represent a promising alternative. Highlights► We performed a 4-year survey in a river long exposed to diuron. ► The ban on diuron resulted in a gradual fall in its concentrations in the river. ► Sediment diuron biodegradation potential decreased throughout the survey. ► Degradation capacities can be an ecological indicator to gauge microbial recovery.

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Section: Did the Ban Of Diuron Lead To A Decrease In The Exposure Levmentioning

confidence: 92%

Freshwater sediment pesticide biodegradation potential as an ecological indicator of microbial recovery following a decrease in chronic pesticide exposure: A case study with the herbicide diuron

Pesce¹,

Margoum²,

Rouard³

et al. 2013

Ecological Indicators

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“…The pmoA gene codes for the alpha subunit of pMMO and was previously shown to be expressed by a mixed community of methanotrophs in soil capable of biodegrading trichloroethylene (TCE) (11). Consequently, biomarkers are becoming increasingly important in bioremediation and monitoring applications for environmental contaminants, with the development of several assays that target specific genes involved in compound degradation (8,12,13). For example, quantification of the cytochrome P450 monooxygenase-encoding gene, ethB, was used as an indicator for the microbial transformation of methyl tert-butyl ether (MTBE) in samples with low levels of contamination (14).…”

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confidence: 99%

Identification of Biomarker Genes To Predict Biodegradation of 1,4-Dioxane

Gedalanga

Pornwongthong

Mora³

et al. 2014

Appl Environ Microbiol

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Bacterial multicomponent monooxygenase gene targets in Pseudonocardia dioxanivorans CB1190 were evaluated for their use as biomarkers to identify the potential for 1,4-dioxane biodegradation in pure cultures and environmental samples. Our studies using laboratory pure cultures and industrial activated sludge samples suggest that the presence of genes associated with dioxane monooxygenase, propane monooxygenase, alcohol dehydrogenase, and aldehyde dehydrogenase are promising indicators of 1,4-dioxane biotransformation; however, gene abundance was insufficient to predict actual biodegradation. A time course gene expression analysis of dioxane and propane monooxygenases in Pseudonocardia dioxanivorans CB1190 and mixed communities in wastewater samples revealed important associations with the rates of 1,4-dioxane removal. In addition, transcripts of alcohol dehydrogenase and aldehyde dehydrogenase genes were upregulated during biodegradation, although only the aldehyde dehydrogenase was significantly correlated with 1,4-dioxane concentrations. Expression of the propane monooxygenase demonstrated a time-dependent relationship with 1,4-dioxane biodegradation in P. dioxanivorans CB1190, with increased expression occurring after over 50% of the 1,4-dioxane had been removed. While the fraction of P. dioxanivorans CB1190-like bacteria among the total bacterial population significantly increased with decrease in 1,4-dioxane concentrations in wastewater treatment samples undergoing active biodegradation, the abundance and expression of monooxygenase-based biomarkers were better predictors of 1,4-dioxane degradation than taxonomic 16S rRNA genes. This study illustrates that specific bacterial monooxygenase and dehydrogenase gene targets together can serve as effective biomarkers for 1,4-dioxane biodegradation in the environment.

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“…DX7 [29], (organophosphorus) by Enterobacter sp. [30]; differential pulse voltammetry has been used for phenol biodegradation by Bacillus amyloliquefaciens WJDB1 [31]; GC‐MS method was used for tributyl phosphate degradation by Bacillus [32]; LC‐MS method was employed for Chlorpyrifos degradation by Enterococcus faecalis [33] and radiorespirometric analyses was utilized for atrazine mineralization by soil bacteria [34]. Among all the techniques used in the detection of pesticide degradation efficiency of bacteria, high‐performance liquid chromatography (HPLC) is reported to be one of the most precise, reliable and convenient techniques for quantification of biodegradation of compounds and to quantify each component.…”

Section: Discussionmentioning

confidence: 99%

Efficacy of soil‐borne Enterobacter sp. for carbofuran degradation: HPLC quantitation of degradation rate

Ekram

Sarker

Rahi³

et al. 2020

J Basic Microbiol

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Excessive use of pesticides in agricultural fields is a matter of great concern for living beings as well as the environment across the world, in particular, the third world countries. Therefore, there is an urgent need to find out an effective way to degrade these hazardous chemicals from the soil in an environment-friendly way. In the current project, a bacterial species were isolated through enrichment culture from carbofuran-supplemented rice-field soil and identified as a carbofuran degrader. The rate of carbofuran degradation by this bacterial species was evaluated using reverse-phase high-performance liquid chromatography (RP-HPLC), which confirmed the ability to utilize as a carbon source up to 4 µg/ml of 99% technical grade carbofuran. The morphological, physiological, biochemical characteristics and phylogenetic analysis of the 16S rRNA sequence showed that this strain belongs to the genus of Enterobacter sp. (sequence accession number LC368285 in DDBJ), and the optimum growth condition for the isolated strain was 37°C at pH 7.0. Moreover, an antibiotic sensitivity test showed that it was susceptible to azithromycin, penicillin, ceftazidime, ciprofloxacin, and gentamycin, and the minimal inhibitory concentration value of gentamycin was 400 μg/ml against the bacteria. It shows beyond doubt from the RP-HPLC quantification that the isolated bacterium has the ability to detoxify carbofuran (99% pure).Finally, the obtained results imply that the isolated strain of Enterobacter can be used as a potential and effective carbofuran degrader for bioremediation of contaminated sites through bioaugmentation. K E Y W O R D S antibiotic sensitivity, biodegradation, carbofuran, cytotoxicity, RP-HPLC How to cite this article: Ekram MA-E, Sarker I, Rahi MS, Rahman MA, Saha AK, Reza MA. Efficacy of soil-borne Enterobacter sp. for carbofuran degradation: HPLC quantitation of degradation rate.

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Estimating the biodegradation of pesticide in soils by monitoring pesticide-degrading gene expression

Cited by 31 publications

References 56 publications

Freshwater sediment pesticide biodegradation potential as an ecological indicator of microbial recovery following a decrease in chronic pesticide exposure: A case study with the herbicide diuron

Freshwater sediment pesticide biodegradation potential as an ecological indicator of microbial recovery following a decrease in chronic pesticide exposure: A case study with the herbicide diuron

Identification of Biomarker Genes To Predict Biodegradation of 1,4-Dioxane

Efficacy of soil‐borne Enterobacter sp. for carbofuran degradation: HPLC quantitation of degradation rate

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