Estimating the Allowable Ammonia Concentrations in Wastewater Treatment Plant Discharge to Ensure Protection of Aquatic Biota

Wade, Abdou Ciss; Maher, Barbara A.; Lawrence, Ian; Davis, N.C.; Zoppou, C.; Bell, Carlos G.

doi:10.1080/09593331908616731

Cited by 9 publications

(8 citation statements)

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“…Waste NH 3 is found in different types of wastewaters, in varying amounts. ,− Concentrations range from <100 mg L –1 for municipal and animal waste to >1 g L –1 for some industrial wastewater . A broadly applicable mitigation process would therefore need to be tolerant of highly variable NH 3 feedstock concentrations.…”

Section: Resultsmentioning

confidence: 99%

“…Citrulline has already been identified as a valuable synthetic target, as a specialty chemical. − However, another attractive concept was that the lysate catalytic system could be used to recycle NH 3 found in agricultural wastewater as a result of fertilizer runoff . Otherwise it leads to algal blooms in rivers and lakes and accounts for the majority of nitrous oxide greenhouse gas formation. , To complete the cycle, citrulline was therefore considered as a plant nitrogen source. The grass family (Poaceae) provides the vast majority of human nutrition, with cereals from this family providing over 60% of global calorific consumption from a worldwide cereal production yield of 2365 million metric tons (2011) that is mostly produced by China, U.S.A., and India .…”

Section: Resultsmentioning

confidence: 99%

“…16−18 However, another attractive concept was that the lysate catalytic system could be used to recycle NH 3 found in agricultural wastewater as a result of fertilizer runoff. 34 Otherwise it leads to algal blooms in rivers and lakes 7 and accounts for the majority of nitrous oxide greenhouse gas formation. 35,36 To complete the cycle, citrulline was therefore considered as a plant nitrogen source.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

“…The reaction of NH 3 with CO 2 to give carbamate is the first step in the industrial production of urea and has been employed with aqueous solutions for CO 2 capture (Scheme ). − In situ utilization of the carbamate to produce value-added chemicals is a strategy for the mitigation and valorization of waste ammonia as well as CO 2 . Biocatalytic routes are particularly attractive due to the aqueous solvents and mild reaction conditions involved; − however, their use is generally limited by high catalyst production costs and the need for stoichiometric amounts of expensive biocatalyst cofactors. − …”

Section: Introductionmentioning

confidence: 99%

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One-Pot Multienzymatic Transformation of NH₃, CO₂, and Ornithine into the Organic Nitrogen Plant Fertilizer Citrulline Using a Single Recombinant Lysate of E. coli

Alissandratos

Hartley

French

et al. 2019

ACS Sustainable Chem. Eng.

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Biocatalytic transformation of carbamate formed readily from CO2 and NH3 provides attractive green routes for mitigation of these important environmental pollutants. Accordingly, a coupled-enzyme system was developed for the one-pot production of citrulline through carbamoylation of ornithine in aqueous solutions of CO2 and NH3. Hyperthermophilic ornithine carbamoyltransferases are produced recombinantly in E. coli with carbamate kinases known to have a propensity for carbamoyl phosphate synthesis. Importantly, in vitro biocatalysis is carried out by E. coli cell lysate prepared through coexpression of the required recombinant enzymes in a single bacterial culture, greatly reducing limitations normally associated with protein production and purification. Acetate kinase that is endogenous in the lysate also recycles the required ATP cofactor, which would otherwise have been required in costly stoichiometric amounts. Recombinant lysates catalyze the production of carbamoyl phosphate with substoichiometric ATP (>300 turnovers) as well as its in situ reaction with ornithine to give citrulline in high yield (>95%) and g L–1 h–1 titers. The system is active over a wide range of NH3 concentrations (2.5 mM – 2 M), and >90% conversions of NH3 may be reached within 1.5 h. Aqueous NH3 used to sequester CO2 gas (10% v/v) may be directly used as the biocatalyst feedstock. In preliminary studies, citrulline is found to be an effective organic nitrogen fertilizer of the wheat grass Brachypodium distachyon. Therefore, lysates described here constitute a cost-effective biocatalytic platform for one-pot production of a promising organic nitrogen fertilizer, under mild reaction conditions, from environmental pollutants as feedstock.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: ■ Results and Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

One-Pot Multienzymatic Transformation of NH₃, CO₂, and Ornithine into the Organic Nitrogen Plant Fertilizer Citrulline Using a Single Recombinant Lysate of E. coli

Alissandratos

Hartley

French

et al. 2019

ACS Sustainable Chem. Eng.

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“…In the case of nitrogen compounds like aqueous ammonia, water eutrophication has been studied using different methods [1,2]. The catalytic technology is an alternative that involves the transformation of the nitrogen-compounds to molecular nitrogen like in catalytic wet air oxidation (CWAO) [3][4][5].…”

Section: Introductionmentioning

confidence: 99%

Catalytic oxidation of aqueous methyl and dimethylamines by activated carbon

et al. 2005

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The catalytic wet air oxidation (CWAO) of methyl and dimethylamines was studied. A commercial peach stones activated carbon and its oxidized and reduced forms were used as catalysts. The observed catalytic behavior is related to the presence of oxygenated surface functional groups on the activated carbons. The N-compounds were selectively oxidized to nitrogen, water and carbon dioxide using these activated carbon catalysts. It is proposed that the quinonic surface groups are responsible for the selective catalytic oxidation of these amines. Carboxylic, lactonic and anhydride groups strongly adsorb the amine compounds producing inhibition of the catalytic activity of the activated carbon in the CWAO process.

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Ammonium oxidizing bacterial populations in South African activated sludge wastewater treatment plants

Welz,

Thobejane,

van Blerk

2023

Water Environment Research

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This is the first study that describes ammonium oxidizing bacterial populations and correlations of these populations with a range of criteria in activated sludge wastewater treatment plants in South Africa. In this study, not only the influent, but also the activated sludge chemistry was comprehensively characterized. Multivariate statistical analyses were used to determine the relative significances of the geographical location (factor: site), wastewater treatment plant process (factor: configuration), seasonality (factor: season), and environmental parameters on the ammonium oxidizing bacterial genera in six municipal activated sludge wastewater treatments plants from two sites (the Cities of Cape Town and Ekurhuleni).The geographical location (site) was significant for selection of the ammonium oxidizing genera (Global ANOSIM R value=0.538, p=0.001). It was established that the inter‐site differences were not climatic in origin, nor related to the composition of the influent, but were rather driven by the activated sludge chemistry. It was found using BEST analysis that the activated sludge ammonia, activated sludge total phosphate and activated sludge total chemical oxygen demand were the most significant (p<0.001) drivers for ammonium oxidizing bacterial selection (ANOSIM Global R value 0.419), and were significantly higher in the activated sludge from the City of Cape Town wastewater treatment plants. Nitrosospira was the most abundant ammonium oxidizing bacterial genus, with notably higher relative and estimated actual abundances in the City of Cape Town wastewater treatment plants than the City of Ekurhuleni wastewater treatment plants. The strong selection of Nitrosospira in the City of Cape Town wastewater treatment plants with higher nutrient concentrations strongly suggests that high concentrations of activated sludge ammonia, activated sludge total phosphate and activated sludge total chemical oxygen demand are key selective drivers for this genus.

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Estimating the Allowable Ammonia Concentrations in Wastewater Treatment Plant Discharge to Ensure Protection of Aquatic Biota

Cited by 9 publications

References 0 publications

One-Pot Multienzymatic Transformation of NH₃, CO₂, and Ornithine into the Organic Nitrogen Plant Fertilizer Citrulline Using a Single Recombinant Lysate of E. coli

One-Pot Multienzymatic Transformation of NH₃, CO₂, and Ornithine into the Organic Nitrogen Plant Fertilizer Citrulline Using a Single Recombinant Lysate of E. coli

Catalytic oxidation of aqueous methyl and dimethylamines by activated carbon

Ammonium oxidizing bacterial populations in South African activated sludge wastewater treatment plants

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Estimating the Allowable Ammonia Concentrations in Wastewater Treatment Plant Discharge to Ensure Protection of Aquatic Biota

Cited by 9 publications

References 0 publications

One-Pot Multienzymatic Transformation of NH3, CO2, and Ornithine into the Organic Nitrogen Plant Fertilizer Citrulline Using a Single Recombinant Lysate of E. coli

One-Pot Multienzymatic Transformation of NH3, CO2, and Ornithine into the Organic Nitrogen Plant Fertilizer Citrulline Using a Single Recombinant Lysate of E. coli

Catalytic oxidation of aqueous methyl and dimethylamines by activated carbon

Ammonium oxidizing bacterial populations in South African activated sludge wastewater treatment plants

Contact Info

Product

Resources

About

One-Pot Multienzymatic Transformation of NH₃, CO₂, and Ornithine into the Organic Nitrogen Plant Fertilizer Citrulline Using a Single Recombinant Lysate of E. coli

One-Pot Multienzymatic Transformation of NH₃, CO₂, and Ornithine into the Organic Nitrogen Plant Fertilizer Citrulline Using a Single Recombinant Lysate of E. coli