Estimating the abundance of toxic Microcystis in the San Francisco Estuary using quantitative real-time PCR

Baxa, Dolores V.; Kurobe, Tomofumi; Ger, Kemal Ali; Lehman, Peggy W.; Teh, Swee J.

doi:10.1016/j.hal.2010.01.001

Cited by 86 publications

(63 citation statements)

References 53 publications

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“…Cyanobacteria are photosynthetic prokaryotes that thrive well in various kinds of habitats ranging from freshwater and marine environments to hot springs and deserts (Duy et al, 2000;Ballot et al, 2003Ballot et al, , 2010Baxa et al, 2010;Bogialli et al, 2013;Chia et al, 2009, Chia andKwaghe, 2015). They are popularly referred to as blue-green algae but their physiological, morphological and metabolic structures clearly identify them as bacteria.…”

Section: Introductionmentioning

confidence: 99%

Risk assessment of cyanobacteria-toxins for small drinking water treatment plants with lake water intakes

Uduma¹,

McBean²,

Gharabaghi³

2017

Int. J. Water Res. Environ. Eng.

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Recent increases in the frequency and severity of toxic algae blooms in freshwater lakes has been a major concern for small communities that rely on them for drinking water supply. A hazard quotient approach to risk characterization is employed to analyze the effectiveness of five conventional treatment methods for removal of cyanotoxins. The application of the method for risk assessment and mitigation is demonstrated for five case studies, including Lake Champlain (Quebec), Coal Lake (Alberta), Butte Lake (Alberta), Kubbani Lake (Nigeria) and Bomo Lake (Nigeria).

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Section: Introductionmentioning

confidence: 99%

Risk assessment of cyanobacteria-toxins for small drinking water treatment plants with lake water intakes

Uduma¹,

McBean²,

Gharabaghi³

2017

Int. J. Water Res. Environ. Eng.

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“…In 2009, the proportions of Microcystis containing the mcyB gene were below 50% in all lakes, varying from 1.53 to 34.52%. Several studies have revealed similar findings (Rita-Kanto et al, 2009;Baxa et al, 2010;Yoshida et al, 2007;Kurmayer & Kutzenberger, 2003). Standard curves were established using four serial dilution (ranging from 2.0 x 10 4 to 20 copies per PCR reaction) of the standard genomic DNA isolated from M. aeruginosa NIVA-CYA 228/1.…”

Section: Both In June 2008 and In March 2009mentioning

confidence: 68%

Quantification of microcystin-producing microcystis in freshwater bodies in the Southern Mozambique using quantitative real time polymerase chain reaction

Pedro¹,

Lie²,

Dacia³

et al. 2013

Afr. J. Biotechnol.

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In the last decades, large number of reported cases of illnesses in Mozambique is related to drinking water. However, only a limited number of studies have focused on aquatic pollution in this country. Cyanobacterial blooms dominated by Microcystis sp are regularly identified in freshwater bodies in Mozambique. Microcystis is known to proliferate in freshwater bodies and produce microcystins which have adverse effects on animals and humans. The aim of this study was to quantify microcystinproducing Microcystis in three different freshwater bodies in Southern Mozambique. TaqMan based real time polymerase chain reaction (PCR) (Taq Nuclease Assay) was used to quantify populations of Microcystis in three aquatic ecosystems in Southern Mozambique. Total Microcystis spp (microcystinproducing and non-producing strains) were quantified in the three selected study areas with the determination of the copy numbers of the phycocyanin (PC) operon. Microcystin-producing gene copy numbers were quantified using specific primer pair, amplifying the mcyB gene. Microcystis mcyB copy numbers varied from 4.2 x 10 6 to 1.6 x 10 9 gene copies /L in 2008, corresponding to 2.15 to 98.55% of total Microcystis, and from 9.6 x 10 7 to 4.5

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“…Because YD is near Juksan weir, the weir may contribute to Microcystis abundance by manipulating hydrological factors. In terms of the water column, toxic Microcystis cells are abundant in surface water [42], which may be attributed to their efficient buoyancy regulation relative to other algal species [43].…”

Section: Temporal and Spatial Dynamics Of Cyanobacteriamentioning

confidence: 99%

Spatial and Temporal Dynamics of Potentially Toxic Cyanobacteria in the Riverine Region of a Temperate Estuarine System Altered by Weirs

Malazarte

Lee

Kim

et al. 2017

Water

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Abstract:The effects of weirs on fish and other biological communities have garnered considerable study, whereas the effects of weirs on community composition of toxic cyanobacteria have not yet been well documented. In this study, temporal and spatial variations in species composition and the abundance of potentially toxic cyanobacteria were investigated in the riverine regions of the temperate Youngsan River estuary, where two weirs have recently been constructed. Four stations were sampled 0.5 m below the surface monthly along the channel of the upper river from May 2014 to April 2015 to explore cyanobacterial composition and abundance, while physicochemical and biological parameters were measured to elucidate possible mechanisms controlling these dynamics. Two stations were located upstream at free-flowing sites, and the other stations were located downstream at impounded sites near the weirs. Twenty-eight cyanobacterial species were identified, seven of which were potentially toxic: Microcystis sp., M. aeruginosa, M. flos-aquae, Dolichospermum sp., Aphanocapsa sp., Oscillatoria sp. and Phormidium sp. Microcystis sp. was the most abundant in June 2014 at the lowest station near the weir. Meanwhile, Phormidium sp. occurred at low abundance throughout the study period, except during the winter months, when its abundance was elevated. The interactive forward selection method highlighted dissolved inorganic nitrogen and zooplankton abundance as explanatory variables for this observed variation, but their effects on cyanobacterial growth are unclear. However, temperature was the major determinant for the temporal variation in cyanobacterial populations. Cluster analysis showed that the downstream stations near the weirs had a high similarity of potentially toxic cyanobacteria. Significantly higher abundance, especially of Microcystis sp., was also recorded at the impounded sites suggesting that the presence of weirs might affect variations in toxic cyanobacterial communities.

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Estimating the abundance of toxic Microcystis in the San Francisco Estuary using quantitative real-time PCR

Cited by 86 publications

References 53 publications

Risk assessment of cyanobacteria-toxins for small drinking water treatment plants with lake water intakes

Risk assessment of cyanobacteria-toxins for small drinking water treatment plants with lake water intakes

Quantification of microcystin-producing microcystis in freshwater bodies in the Southern Mozambique using quantitative real time polymerase chain reaction

Spatial and Temporal Dynamics of Potentially Toxic Cyanobacteria in the Riverine Region of a Temperate Estuarine System Altered by Weirs

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