In previous work, we developed a calibration apparatus for use with contact surface thermometers. In this apparatus, the temperature of the upper surface of a copper cube was used as reference surface temperature. In this new study, uncertainty components due to environmental conditions in the measurement space were estimated by measuring the temperature of the upper surface of the calibration apparatus and the output of contact surface thermometers while varying ambient temperature and air flow speed. It was found that the temperature of the upper surface of the calibration apparatus and the output of the contact surface thermometer increased with increasing ambient temperature. The uncertainty in our previous work due to the effect of the ambient temperature was estimated to be 0.01 • C, based on the ±1 • C variance of the ambient temperature in the lab room. We also found that the measured values tended to decrease with increasing the air flow speed around the calibration apparatus, but the effect of the air flow speed depends on the size of the contact surface thermometer. The effect of the air flow speed around the calibration apparatus was 0.07 • C using Model A of the contact surface thermometer, which has a 10 cm height, on the basis of the stability of air flow in the lab room being within ≪2 m s −1 .