Estimating size and diversity of nitrifying communities in deodorizing filters using PCR and immunofluorescence

Malhautier,; Degrange,; Guay,; Degorce‐Dumas,; Bardin, Marc; Cloirec,

doi:10.1046/j.1365-2672.1998.00492.x

Cited by 13 publications

(6 citation statements)

References 25 publications

(26 reference statements)

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“…It should be noted that the MPN-Griess method has been criticized for its potential underestimation of bacterial populations (Belser, 1979). When compared with the immunofluorescence technique or MPN-PCR method, it is considered to lead to an insignificant underestimation of cell counts (Feray et al, 1999;Malhautier et al, 1998). However, the MPN-Griess technique is still considered to be advantageous for enumerating the autographic nitrifying community because many defects may exist in other methods (Feray et al, 1999;Konuma et al, 2001).…”

Section: Abundance Of Nitrifying Bacteriamentioning

confidence: 99%

Nitrification and inorganic nitrogen distribution in a large perturbed river/estuarine system: the Pearl River Estuary, China

Dai

Wang²,

Guo³

et al. 2008

Biogeosciences

224

176

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Abstract. We investigated the spatial distribution and seasonal variation of dissolved inorganic nitrogen in a large perturbed estuary, the Pearl River Estuary, based on three cruises conducted in winter (January 2005), summer (August 2005) and spring (March 2006). On-site incubation was also carried out for determining ammonium and nitrite oxidation rates (nitrification rates). We observed a year-round pattern of dramatic decrease in NH , accompanied by extremely high concentrations of ammonia (up to >800 µmol L −1 ) and nitrate (up to >300 µmol L −1 ). In summer, the upper estuary showed higher nitrification rates (ammonia oxidation rate ∼1.5-33.1 µmol N L −1 d −1 , nitrite oxidation rate ∼0.6-32.0 µmol N L −1 d −1 ) with lower concentrations of ammonia (<350 µmol L −1 ) and nitrate (<120 µmol L −1 ). The Most Probable Number test showed relatively lower nitrifier abundance in summer at most sampling stations, indicating a greater specific nitrification rate per cell in the warm season. Temperature appeared to control nitrification rates to a large degree in different seasons. Spatial variability of nitrification rates appeared to be controlled by a combination of many other factors such as nutrient concentrations, nitrifier abundance and dissolved oxygen (DO) concentrations. In addition to aerobic respiration, nitrification contributed significantly to the consumption of DO and production of free CO 2 at upper estuary. Nitrification-induced consumption accounted for up to approximately one third of the total waCorrespondence to: M. Dai (mdai@xmu.edu.cn) ter column community DO consumption in the upper estuary during the surveyed periods, boosting environmental stress on this large estuarine ecosystem.

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Section: Abundance Of Nitrifying Bacteriamentioning

confidence: 99%

Nitrification and inorganic nitrogen distribution in a large perturbed river/estuarine system: the Pearl River Estuary, China

Dai

Wang²,

Guo³

et al. 2008

Biogeosciences

224

176

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“…Therefore, combining adsorption and biofiltration technologies is sometimes believed to be a very promising alternative. A few studies suggest the usage of activated carbon alone as biofilter support to treat gaseous effluents containing VOCs (Graham, 1996;Malhautier et al, 1998;(Medina et al, 1995)). As a matter of fact, modeling of biofiltration indicates that highly adsorptive media, such as AC, would provide treatment advantages over moderately adsorptive media, such as compost or peat (Hodge et al, 1992).…”

Section: Introductionmentioning

confidence: 99%

Biofiltration of a mixture of volatile organic compounds on granular activated carbon

Aizpuru

Malhautier

Roux

et al. 2003

Biotech & Bioengineering

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The performance of a biofilter packed with Active Carbon (AC) was evaluated. The effluent (alcohol, ketones, esters, aromatic and chlorinated compounds) treated was a representative mixture of most common industrial emissions. To achieve a better knowledge of multicomponent adsorption mechanisms, and to underline the interest of inoculating AC, a control abiotic humidified filter had been operated in the same conditions as the biofilter. For a load of 110 g VOC m(-3) AC h(-1), after 55 days of operation, the removal efficiency was higher in the biotic than in the abiotic filter (85% vs 55%, respectively). Moreover, in the biofilter, at steady state, the elimination of all compounds was almost complete except for chlorinated compounds and p-xylene (removal efficiency of 25% and 64%, respectively). The microbial colonization of AC involved a decrease of the adsorption sites accessibility and enhanced the treatment of VOCs (volatile organic compounds) having a lower affinity for activated carbon. Moreover, while aromatic compounds and MIBK were eliminated along the overall height of the biofilter, pollutants with reduced affinity for AC, such as methanol, acetone, and halogenated compounds were only treated on the second half of the reactor. Thus, the affinity for activated carbon was an important parameter controlling the biodegradation process. Nevertheless, the use of AC as packing material in biofilters treating complex mixtures of VOCs is limited. Actually, similar removal efficiency could be reached, in the same conditions, for a biofilter packed with granular peat. Furthermore, for the biofilter packed with AC, the column height necessary to remove biodegradable compounds, with reduced affinity for the support, was important.

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“…Screening methods for the selection of the appropriate type of PAC as well as selection guidelines for the packing material have been previously established [13,20,21]. The present study only focused on evaluating removal efficiency, removal capacity, removal kinetics and reaction mechanisms.…”

Section: Synthesis Of Fe(no 3 ) 3 and H 3 Po 4 Combined Pelletized Acmentioning

confidence: 99%

Synthesis of Fe(NO3)3 and H3PO4 Combined Pelletized Activated Carbon Composites for Removal of Ultrafine Pollutant Gases (NH3, CO2 and H2S)

et al. 2019

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In the present study, the potential of pelletized activated carbon (PAC) inoculated with Fe(NO3)3 and H3PO4 as packing material for NH3, CO2 and H2S removal from mixed waste gases was evaluated. Broad tests were conducted to determine the factors affecting the removal of high concentrations of NH3. The removal characteristics, removal efficiency, and removal capacity of the system were evaluated. The results of bed depth service time experiment suggested that the removal of NH3, CO2 and H2S results from physical adsorption of the gases by pelletized activated carbon. The vigorous steady state of physical adsorption lasted for approximately 4 h. After the system achieved equilibrium, the pelletized activated carbon bed exhibited high adaptation to shock loading, elevated temperatures, and high flow rates. The results also demonstrated that the removal of NH3 was not affected by the presence of H2S. Gas retention time was a crucial factor affecting system performance. A retention time of ≥ 65s was required to obtain NH3 removal efficiencies of ≥ 15 %. The acute loading of NH3 for system was 4.2 gN/m 3 /h and the maximal loading was 16.2 gN/m 3 /h. The results of this study can be used as a guide for the design and operation of industrial-scale systems in the future.

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Estimating size and diversity of nitrifying communities in deodorizing filters using PCR and immunofluorescence

Cited by 13 publications

References 25 publications

Nitrification and inorganic nitrogen distribution in a large perturbed river/estuarine system: the Pearl River Estuary, China

Nitrification and inorganic nitrogen distribution in a large perturbed river/estuarine system: the Pearl River Estuary, China

Biofiltration of a mixture of volatile organic compounds on granular activated carbon

Synthesis of Fe(NO3)3 and H3PO4 Combined Pelletized Activated Carbon Composites for Removal of Ultrafine Pollutant Gases (NH3, CO2 and H2S)

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