Estimating puma<i>Puma concolor</i>population size in a human-disturbed landscape in Brazil, using DNA mark–recapture data

Miotto, Renata Alonso; Cervini, Marcelo; Kajin, Maja; Begotti, Rodrigo Anzolin; Galetti, Pedro Manoel

doi:10.1017/s0030605312000841

Cited by 33 publications

(29 citation statements)

References 68 publications

(110 reference statements)

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“…The neighbor-joining-tree recovered these three jaguar samples within the group of Panthera onca (Figure 1), reinforcing that the method used was able to correctly identify the species who deposited the faeces collected. The ATP6 and CytB gene have been previously used for felid identification (Chaves et al 2012, Miotto et al 2014, Wultsch et al 2016, and proved to be a powerful molecular marker for this group of carnivores.…”

Section: Resultsmentioning

confidence: 99%

“…In this study, we used a non-invasive DNA analysis to identify felid scats collected in the SVU, a recognized method for species identification (Farrel et al 2000, Haag et al 2009, Chaves et al 2012, Miotto et al 2014). Our results indicated at least three individuals (two females and one male) inside SVU, thus confirming the occurrence of this key top predator in this important protected area.…”

Section: Introductionmentioning

confidence: 99%

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Re-discovering jaguar in remaining coastal Atlantic Forest in southeastern Brazil by non-invasive DNA analysis

et al. 2017

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Jaguar populations have been declining in Brazil mostly due to habitat loss and fragmentation, conflict with humans, poaching and reduction of prey. This is dramatically true in the Atlantic Forest, where occurrence of this large felid is now restricted to very few remaining areas. We used a non-invasive DNA analysis to search through felid scats collected in the Santa Virginia Unit (SVU) of the Serra do Mar State Park, to test for the potential presence of jaguar there. Our results indicated at least three individuals (two females and one male) inside SVU, thus confirming at least temporary presence of this top predator in this important protected area. It is now crucial to intensify studies in that area and surroundings, to evaluate the status of these individuals and identify conservation needs to urgently improve the prospects for the establishment of a resident population, allowing it to expand to adjoining units of the Serra do Mar State Park and Serra da Bocaina National Park. Keywords: Panthera onca, fecal DNA, Santa Virginia Unit, Serra do Mar State ParkRedescobrindo a onça-pintada em um remanescente de Mata Atlântica costeira no sudeste do Brasil por análise não-invasiva de DNA Resumo: As populações de onça-pintada têm sofrido declínio populacional devido a perda e fragmentação de habitat, por conflito com humanos, através da caça e pela redução da disponibilidade de suas presas. Isso é particularmente drástico no bioma Mata Atlântica, onde a ocorrência desse grande felino está atualmente restrita a poucos remanescentes. Utilizamos análise de DNA a partir de amostras de fezes para verificar a pontencial presença da espécie no Núcleo Santa Virgínia (NSV) do Parque Estadual da Serra do Mar. Os resultados indicaram a ocorrência pelo menos esporádica de três indivíduos (duas fêmas e um macho) no interior do NSV. Com isso, se torna crucial agora intensificar estudos naquela unidade e em áreas adjacentes, para avaliar a situação desses indivíduos e identificar as necessidades mais urgentes de conservação para melhorar a probabilidade de estabelecimento de uma população residente da espécie, permitindo que ela possa expandir para unidades de conservação vizinhas, como outros núcleos do Parque Estadual da Serra do Mar e o Parque Nacional Serra da Bocaina.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Re-discovering jaguar in remaining coastal Atlantic Forest in southeastern Brazil by non-invasive DNA analysis

et al. 2017

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“…In order to confirm if the samples belonged to Puma concolor, we amplified a fragment of 146 bp from cytochrome b of mitochondrial DNA using the primers described by Farrel et al [24], as previously used by Miotto et al [17,25,26] for cougar identification. We adopted the PCR protocol described by Miotto et al [25] using a Veriti 96-Well Thermal Cycler (Applied Biosystems, Foster City, CA, USA).…”

Section: Molecular Species Confirmationmentioning

confidence: 99%

Evidence of Recent Fine-Scale Population Structuring in South American Puma concolor

Saranholi¹,

Chávez-Congrains²,

Galetti³

2017

Diversity

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Habitat loss and fragmentation are considered the major treats to worldwide biodiversity. Carnivores in particular can be more sensitive to landscape modification due to their low occurrence density and large home ranges. Population structuring of Puma concolor has been already reported as a consequence of extensive human activities in the North American continent. Here, we investigated the occurrence of fine-scale population structuring in the South American cougar population, contrasting two conservation areas immersed in a highly human-fragmented landscape dominated by the presence of sugar cane monoculture, roads, and urbanization, including a series of dams in the Tietê River which enlarges its water body. Seven microsatellites were amplified using non-invasive DNA obtained from fecal samples. We conducted genetic clustering analyses using Bayesian and factorial components. We also performed genetic differentiation analyses by fixation indices (F st and D est ). Two genetic clusters represented by individuals from each area were found, indicating the occurrence of gene flow reduction between the areas. The intense human-induced landscape modification-which includes the Tietê River water body enlargement, imposing physical barriers to the movement of the individuals-could explain the gene flow reduction. Increasing connectivity among the preserved areas can mitigate such effects, and the creation of corridors or further management actions such as individual translocation to ensure gene flow in the highly-modified landscape may be essential for maintaining the genetic and demographic health of the species and its long-term persistence.

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“…They distribution ranged from northern British Columbia to southern Chile and Argentina, but hunting pressure and changes in land management practices have restricted their range, isolating the populations to patched areas (Currier, 1983;Mills, 2007;Miotto, Cervini, Kajin, Begotti, & Galetti, 2014). However, Ruiz-García, Pacheco, and Álvarez (2009) yielded strong results in favour of a unique gene pool of pumas in north-western South America, in contrast to the traditional morphology and morphometric classifications, which had identified a considerable number of puma subspecies in this region of Latin America.…”

Section: Introductionmentioning

confidence: 99%

Papillary architecture of the lingual surface in the puma (Puma concolor)

Erdoğan

Villar

König

et al. 2017

Anat Histol Embryol

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SummaryThis research presents the first anatomical description of the tongue and lingual papillae of the mountain lion (puma). The tongues of three adult male pumas were used in this study. The tongues were dissected and studied firstly by gross and stereomicroscopy. Samples of each part were processed by study with scanning electron microscopy. The margins of the lingual apex were surrounded by numerous filiform papillae, which had a bulky papillary body and a bifurcated tip. On the dorsal surface of the lingual apex, filiform papillae were remarkably pointed and had many secondary projections, which emerged from the base of the main papilla. In the rostral half of the lingual body, filiform papillae were longer, cylindrical and had blunt tips. On the caudal half of the lingual body, filiform papillae gave place to conical ones exhibiting a pointed tip. The fungiform papillae were scattered on the whole dorsal surface of the tongue.On each lateral half of the tongue, four circumvallate papillae were observed and each circumvallate papilla was surrounded by thick and horseshoe-like annular pad, which were composed by pointed conical papillae on the caudal border of the lingual body.The dorsal surface of the circumvallate papilla was covered by many finger-like protrusion, and the tip of each protrusion had a central orifice. Anatomical distribution of lingual papillae was different to other carnivores and represents the adaptation to the feeding habits of this mammal. General morphology of the lingual structures was similar to those of the tiger.

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Estimating pumaPuma concolorpopulation size in a human-disturbed landscape in Brazil, using DNA mark–recapture data

Cited by 33 publications

References 68 publications

Re-discovering jaguar in remaining coastal Atlantic Forest in southeastern Brazil by non-invasive DNA analysis

Re-discovering jaguar in remaining coastal Atlantic Forest in southeastern Brazil by non-invasive DNA analysis

Evidence of Recent Fine-Scale Population Structuring in South American Puma concolor

Papillary architecture of the lingual surface in the puma (Puma concolor)

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