Estimating Factors Related to Fluoroquinolone Resistance Based on One Health Perspective: Static and Dynamic Panel Data Analyses From Europe

Zhang, Dandan; Cui, Youwen; Zhang, Xiaopeng

doi:10.3389/fphar.2019.01145

Cited by 10 publications

(15 citation statements)

References 43 publications

(47 reference statements)

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“…This excessive and inappropriate use is accelerating the emergence of antimicrobial resistance (AMR), which makes infections induced by microorganisms difficult to treat and sometimes incurable [3]. According to the World Health Organization (WHO), the AMR has become a global health concern since expected fatalities caused by multi-drug resistance will surpass 10 million by 2050 [4,5].…”

Section: Introductionmentioning

confidence: 99%

Investigation of a Truncated Aptamer for Ofloxacin Detection Using a Rapid FRET-Based Apta-Assay

et al. 2020

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In this work, we describe the use of a new truncated aptamer for the determination of ofloxacin (OFL), being a principal quinolone commonly used in both human and animal healthcare. Since the affinity of a 72-mer ssDNA sequence has been previously described without further investigations, this paper demonstrates the first computational prediction of the binding motif between this aptamer and OFL through in silico molecular docking studies. Besides, we suggest the application of the characterized recognition mechanism in a simple FRET (Förster Resonance Energy Transfer) pattern for the rapid aptasensing of the quinolone of interest. Accordingly, our approach harnesses the fluorescence quenching of the fluorescein-tagged aptamer (FAM-APT) induced by its partial hybridization to a tetramethyl rhodamine-labelled complementary ssDNA (TAMRA-cDNA). In such a structure, dye labels brought into close proximity act as a FRET pair. Upon ofloxacin addition, an affinity competition occurs to form a more stable FAM-APT/OFL complex, thus unquenching the FAM-APT signal. Interestingly, the recovered fluorescence intensity was found to correlate well with the antibiotic’s concentrations in the range of 0.2–200 μM in HEPES buffer, with a linear response that ranged between 0.2 and 20 μM. The rapid apta-assay achieved limits of detection and quantification of 0.12 and 0.40 μM, respectively. The truncated aptamer has also shown an improved specificity toward OFL than other quinolones, compared to the original full-length aptamer described in previous works. Finally, the practical application of the developed apta-assay was successfully confirmed to detect OFL quinolone in spiked milk samples, with satisfactory recoveries ranging between 97.4% and 111.4%.

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Section: Introductionmentioning

confidence: 99%

Investigation of a Truncated Aptamer for Ofloxacin Detection Using a Rapid FRET-Based Apta-Assay

et al. 2020

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“…Take statistical potential sources of bias seriously. Regression models can reveal an insignificant or even negative relationship between antimicrobial consumption and AMR [ 56 ]. While this may indeed be reflective of reality, such results can also arise from omitted variable bias (e.g., an instance in which wealthier populaces are more likely to be able to afford antimicrobials and simultaneously more likely to have well-funded WASH infrastructures which reduce the spread of resistant pathogens).…”

Section: Recommendations For Future Analysismentioning

confidence: 99%

“…AMR surveillance data for external validation (Opatowski et al, 2020) [ 61 ] The risk of human AMR acquisition in a representative Asian population is modelled using this multi-level causal model Panel regression models Data on AMR and AMU in humans and food-producing animals, as well as other relevant covariates, are collected over time and for multiple geographical units (e.g., countries or administrative areas). Human AMR is regressed against these covariates using a method such as fixed effects (static) or system GMM (dynamic) Country-level surveillance data on AMR and AMU in humans and food-producing animals over time, as well as country-level data on appropriate controls, e.g., medical staffing, portion of employment in agriculture, population density, average annual temperature, and income per capita

(Zhang, Cui and Zhang, 2018) [ 56 ] Where lnAMR is log AMR prevalence in humans, i denotes country and t denotes year; MS denotes the number of medical staff and VP denotes the number of veterinary professionals; and VAMC and HAMC denote veterinary and human antimicrobial consumption Fluoroquinolone resistance in E. coli and P. aeruginosa is regressed against a series of country-level factors for a panel of European countries …”

Section: Selected Studies Relevant To the Link Between Animal Antimicrobial Use And Human Antimicrobial Resistancementioning

confidence: 99%

Quantifying the Relationship between Antibiotic Use in Food-Producing Animals and Antibiotic Resistance in Humans

et al. 2022

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It is commonly asserted that agricultural production systems must use fewer antibiotics in food-producing animals in order to mitigate the global spread of antimicrobial resistance (AMR). In order to assess the cost-effectiveness of such interventions, especially given the potential trade-off with rural livelihoods, we must quantify more precisely the relationship between food-producing animal antimicrobial use and AMR in humans. Here, we outline and compare methods that can be used to estimate this relationship, calling on key literature in this area. Mechanistic mathematical models have the advantage of being rooted in epidemiological theory, but may struggle to capture relevant non-epidemiological covariates which have an uncertain relationship with human AMR. We advocate greater use of panel regression models which can incorporate these factors in a flexible way, capturing both shape and scale variation. We provide recommendations for future panel regression studies to follow in order to inform cost-effectiveness analyses of AMR containment interventions across the One Health spectrum, which will be key in the age of increasing AMR.

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“…An excellent example of an increasing issue with antibiotic resistance over the years is the quinolones, to which resistance emerges as soon as they are introduced (Witzany et al, 2020; Zhang et al, 2019). The mechanism of quinolone action is the inhibition of two essential bacterial type II topoisomerase enzymes, DNA gyrase and DNA topoisomerase IV (Hooper, 1998).…”

Section: Introductionmentioning

confidence: 99%

Antibiotic-induced recombination in bacteria requires the formation of double-strand breaks

Nath

Roizman

Rolff

et al. 2022

Preprint

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Recombination is an essential process in bacterial drug resistance evolution. Fluoroquinolones, an important class of antibiotics, have been reported to stimulate recombination in Escherichia coli. Bacteria’s sublethal exposure to several other antibiotics from different families, including strong inducers of the SOS stress response and antimicrobial peptides, does not affect recombination rates. Here we ask why fluoroquinolones, and no other antimicrobials, increase recombination rates. We found that the fluoroquinolone ciprofloxacin stimulated recombination in E. coli not only via increased SOS induction but also by DNA double-strand breaks (DSBs). On this basis, we tested whether other antimicrobials, known for their ability to cause double-strand breaks, such as mitomycin C and bleomycin, also affect recombination rates. Indeed, both antibiotics also increased recombination frequency. We found a positive correlation between the number of DNA double-strand breaks and the recombination frequency. These results imply that recombination, at least in Gram-negative bacteria, is conservative, and even if the substrate of recombination is available, the basal recombination rate is low, and it is more intended to repair than to generate diversity. We also show that only antibiotics that cause DNA double-strand breaks can increase genetic diversity via recombination and increase the risk for antibiotic resistance.

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Estimating Factors Related to Fluoroquinolone Resistance Based on One Health Perspective: Static and Dynamic Panel Data Analyses From Europe

Cited by 10 publications

References 43 publications

Investigation of a Truncated Aptamer for Ofloxacin Detection Using a Rapid FRET-Based Apta-Assay

Investigation of a Truncated Aptamer for Ofloxacin Detection Using a Rapid FRET-Based Apta-Assay

Quantifying the Relationship between Antibiotic Use in Food-Producing Animals and Antibiotic Resistance in Humans

Antibiotic-induced recombination in bacteria requires the formation of double-strand breaks

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