Estimating Dynamic Cellular Morphological Properties via the Combination of the RTCA System and a Hough-Transform-Based Algorithm

Zhang, Lejun; Yang, Ye; Dhar, Rana; Deng, Jinsong; Tang, Huifang

doi:10.3390/cells8101287

Cited by 11 publications

(8 citation statements)

References 39 publications

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“…In E-Plate 16, DU145 cells were cultured at 5 × 10 4 cells/well, and the “cell index” parameter (which represents cell status) was automatically recorded using an RTCA System every 15 min ( Zhang et al, 2019a ). After adherence to the plate, the cells were treated with sophocarpine, and the “cell index” was continued to be recorded every 15 min for about 40 h.…”

Section: Methodsmentioning

confidence: 99%

Sophocarpine inhibits tumor progression by antagonizing the PI3K/AKT/mTOR signaling pathway in castration-resistant prostate cancer

Wang

Shi

Han

et al. 2022

PeerJ

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Objective The objective of this study was to investigate the inhibitory effect of sophocarpine on the progression of castration-resistant prostate cancer (CRPC) and the underlying molecular mechanism. Methods DU145 and PC3 cells (two CRPC cell lines), incubated with different concentrations of sophocarpine, were used. Cell Counting Kit-8 assay, real-time cellular analysis, and colony formation assay were conducted to evaluate the proliferation of CRPC cells. Cytometry flow analysis was performed to evaluate the apoptosis rate of CRPC cells. Wound healing and Transwell invasion assays were performed and the levels of the epithelial-mesenchymal transition (EMT)-related proteins were determined to analyze cell migration and invasion abilities. A xenografted tumor model of nude mice was used to examine the anti-cancer effect of sophocarpine on CRPC. Western blotting was performed to evaluate the activities of the PI3K/AKT/mTOR signaling pathway both in cells and tumor tissues. Results In vitro tests showed that sophocarpine suppressed the proliferation of CRPC cells, reduced the migration and invasion abilities, and increased the apoptosis rate. In vivo, sophocarpine decreased the weight and volume of tumor tissues. Mechanically, sophocarpine exerted its anti-cancer effects by inactivating PI3K/AKT/mTOR signaling. Conclusion Sophocarpine inhibited the progression of CRPC by downregulating the PI3K/AKT/mTOR signaling pathway and showed a potential to be an anti-cancer agent against CRPC.

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Section: Methodsmentioning

confidence: 99%

Sophocarpine inhibits tumor progression by antagonizing the PI3K/AKT/mTOR signaling pathway in castration-resistant prostate cancer

Wang

Shi

Han

et al. 2022

PeerJ

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“…Transfected cells (100 µL) were seeded onto 96-well plates, and then incubated for 30 min. After incubation, samples were analyzed by a real-time cell analysis (RTCA) workbench (ACEA Biosciences, USA) [ 26 ]. The number of cells was measured at 15 min intervals for 72 h.…”

Section: Methodsmentioning

confidence: 99%

Necdin, one of the important pathway proteins in the regulation of osteosarcoma progression by microRNA-200c

Wang

et al. 2022

Bioengineered

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MicroRNA-200c (miR-200c) generally acts as a tumor suppressor in multiple cancer types and a promising therapeutic target in tumorigenesis. However, only a few studies have explained the role of miR-200c in the development of osteosarcoma (OS). In this study, we investigated the role of miR-200c in OS progression and identified the regulatory pathway protein NDN involved in inhibiting the occurrence and development of OS. Firstly, we found that miR-200c is downregulated in OS cells and tissues. As well, in vitro and in vivo experiments showed that upregulating miR-200c inhibits the proliferation, invasion, metastasis of Saos-2 cells, promotes the apoptosis of Saos-2 cells and suppresses tumor growth in mice, indicating miR-200c plays a major role in regulating the OS progression. Furthermore, bioinformatics analysis showed that an anti-tumor protein, necdin (NDN), might be a potential target by miR-200c. To verify this hypothesis, we measured the expression level of NDN in OS cells and tissues and found NDN is downregulated, suggesting NDN is functional in OS progression. Moreover, we found that the expression levels of NDN and miR-200c in in vivo and in vitro experiments were positively correlated. However, the results of dual-luciferase reporter gene experiment showed miR-200c does not directly act on the 3ʹ untranslated region (UTR) of NDN gene, indicating that NDN might be an important pathway protein which regulates OS progression in the presence of miR-200c. Therefore, miR-200c/NDN could be potential targets for developing effective treatment against OS.

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“…Since most suspended cells have a spherical or nearly spherical shape, after image segmentation is completed, the contour of the cell image can be extracted by the geometric figure extraction algorithm. Common edge detection algorithms include the Sobel algorithm 28 , 29 , Canny algorithm 30 , Hough transform 31 , 32 , snake model 33 , etc. Huang et al 34 applied the Canny edge detection algorithm to automatically detect the contour of the chorion and cytoplasm of zebrafish oocytes and the edge of a microinjection needle in their experiment and adopted chord midpoint Hough transformation (CMHT) to improve the efficiency and accuracy of ellipse detection in the images.…”

Section: Oocyte Micromanipulationmentioning

confidence: 99%

Recent advances in critical nodes of embryo engineering technology

Ma¹,

Gu²,

Chen³

et al. 2021

Theranostics

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The normal development and maturation of oocytes and sperm, the formation of fertilized ova, the implantation of early embryos, and the growth and development of foetuses are the biological basis of mammalian reproduction. Therefore, research on oocytes has always occupied a very important position in the life sciences and reproductive medicine fields. Various embryo engineering technologies for oocytes, early embryo formation and subsequent developmental stages and different target sites, such as gene editing, intracytoplasmic sperm injection (ICSI), preimplantation genetic diagnosis (PGD), and somatic cell nuclear transfer (SCNT) technologies, have all been established and widely used in industrialization. However, as research continues to deepen and target species become more advanced, embryo engineering technology has also been developing in a more complex and sophisticated direction. At the same time, the success rate also shows a declining trend, resulting in an extension of the research and development cycle and rising costs. By studying the existing embryo engineering technology process, we discovered three critical nodes that have the greatest impact on the development of oocytes and early embryos, namely, oocyte micromanipulation, oocyte electrical activation/reconstructed embryo electrofusion, and the in vitro culture of early embryos. This article mainly demonstrates the efforts made by researchers in the relevant technologies of these three critical nodes from an engineering perspective, analyses the shortcomings of the current technology, and proposes a plan and prospects for the development of embryo engineering technology in the future.

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Estimating Dynamic Cellular Morphological Properties via the Combination of the RTCA System and a Hough-Transform-Based Algorithm

Cited by 11 publications

References 39 publications

Sophocarpine inhibits tumor progression by antagonizing the PI3K/AKT/mTOR signaling pathway in castration-resistant prostate cancer

Sophocarpine inhibits tumor progression by antagonizing the PI3K/AKT/mTOR signaling pathway in castration-resistant prostate cancer

Necdin, one of the important pathway proteins in the regulation of osteosarcoma progression by microRNA-200c

Recent advances in critical nodes of embryo engineering technology

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